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A kind of nano silkworm chrysalis protein powder and preparation method thereof

A silkworm chrysalis protein powder, nanotechnology, applied in the field of silkworm chrysalis protein powder, can solve the problems of protein loss, high protein residue, deterioration, etc., to avoid the loss of nutrients, excellent nutritional value, and solve the effect of color and taste

Active Publication Date: 2020-10-23
NANTONG LOVER APPL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art adopts isoelectric point and organic solvent to realize the deodorization of silkworm chrysalis protein, wherein the process of dissolving pupa protein with lye and then controlling the isoelectric point precipitation is too long and the effect is not good; the use of organic solvent impregnation method can only remove lipophilic, Odor substances with low boiling point, unsatisfactory decolorization and deodorization effect
In the current reports on the refining of silkworm chrysalis protein, high temperature and long-term solvent extraction is mostly used to obtain a higher degreasing rate. The degreasing solvent used is highly toxic and has a high residue in the protein; Complete, severe protein loss, protein structure destruction, excessive degradation, and deterioration of protein processing properties

Method used

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  • A kind of nano silkworm chrysalis protein powder and preparation method thereof
  • A kind of nano silkworm chrysalis protein powder and preparation method thereof
  • A kind of nano silkworm chrysalis protein powder and preparation method thereof

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Effect test

Embodiment 1

[0043] A preparation method of nano silkworm chrysalis protein powder, comprising the following steps:

[0044](1) The degreased pupae were first washed with tap water and then washed with pure water, then added to water, then subjected to wall breaking treatment, and then ultrasonic treatment to obtain the treatment solution; the speed of wall breaking treatment was 50000rpm, and the time was 35 minutes; the defatted pupae and water The mass ratio is 1:25; the power of ultrasonic treatment is 700W, and the frequency is 40KHz;

[0045] (2) Add adsorbent activated carbon to the treatment liquid, and conduct adsorption treatment at 80°C; then press filter to obtain the filtrate; the amount of activated carbon added is 2% of the mass of the treatment liquid;

[0046] (3) Add papain to the filtrate, and carry out enzymolysis under vibration conditions to obtain an enzymolysis solution; the dosage of papain is 2500 activity units / mL filtrate, the enzymolysis temperature is 48°C, th...

Embodiment 2

[0052] A preparation method of nano silkworm chrysalis protein powder, comprising the following steps:

[0053] (1) The degreased pupae were first washed with tap water and then washed with pure water, then added to water, then subjected to wall breaking treatment, and then ultrasonic treatment to obtain the treatment solution; the speed of wall breaking treatment was 45000rpm, and the time was 30 minutes; the defatted pupae and water The mass ratio is 1:30; the power of ultrasonic treatment is 500W, and the frequency is 50KHz;

[0054] (2) Add adsorbent activated carbon to the treatment liquid, and conduct adsorption treatment at 80°C; then press filter to obtain the filtrate; the amount of activated carbon added is 2% of the mass of the treatment liquid;

[0055] (3) Add papain to the filtrate, and carry out enzymolysis under vibration conditions to obtain the enzymolysis solution; the dosage of papain is 3000 activity units / mL filtrate, the enzymolysis temperature is 50°C, ...

Embodiment 3

[0059] A preparation method of nano silkworm chrysalis protein powder, comprising the following steps:

[0060] (1) The degreased pupae were first washed with tap water and then washed with pure water, then added to water, then subjected to wall breaking treatment, and then ultrasonic treatment to obtain the treatment liquid; the speed of wall breaking treatment was 50000rpm, and the time was 40 minutes; the defatted pupae and water The mass ratio is 1:30; the power of ultrasonic treatment is 800W, and the frequency is 30KHz;

[0061] (2) Add adsorbent activated carbon to the treatment liquid, and conduct adsorption treatment at 85°C; then press filter to obtain the filtrate; the amount of activated carbon added is 3% of the mass of the treatment liquid;

[0062] (3) Add papain to the filtrate, and carry out enzymolysis under vibration conditions to obtain the enzymolysis solution; the dosage of papain is 3000 activity units / mL filtrate, the enzymolysis temperature is 50°C, th...

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Abstract

The invention discloses nano silkworm pupa protein powder and a preparation method thereof. The preparation method comprises the following steps: adding degreased pupas into water, and performing wall-breaking treatment to obtain treating fluid; adding an adsorbent into the treating fluid to perform adsorption treatment; performing filter pressing treatment to obtain filtrate; adding neutral protease into the filtrate to perform enzymolysis, thereby obtaining enzymatic hydrolysate; enabling enzymatic hydrolysate to pass through ion exchange resin and then concentrating to obtain concentrate; and performing supergravity treatment on the concentrate and then performing freeze-drying, thereby obtaining the nano silkworm pupa protein powder. The nano silkworm pupa protein powder disclosed by the invention contains eighteen amino acids which are balanced in proportion, and is full-rate protein; and especially, the nano silkworm pupa protein powder provided by the invention contains 40% of amino acids or more which are necessary to a human body, and has an important nutrient value and a practical application meaning.

Description

technical field [0001] The invention relates to a silkworm chrysalis protein powder, in particular to a nano silkworm chrysalis protein powder and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of science and technology and the continuous improvement of people's quality of life requirements, the various metamorphosis stages of silkworms have become more and more widely used in food, medicine, health care, etc., not only making some by-products of silkworm production in the past Even the wastes are developed and utilized, and in some respects the output value created is much higher than that of sericulture and silk reeling itself. At present, most silkworm chrysalis are basically used as feed and fertilizer. Not only the resource utilization rate is low, but also the meat quality of livestock, poultry and fish raised by silkworm chrysalis has pupae smell, which seriously affects the quality of related products. Therefo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/17A23L5/20A23L5/30A23L5/40A23J1/02A23J3/04A23J3/34
CPCA23J1/02A23J3/04A23J3/341A23V2002/00A23L5/25A23L5/273A23L5/30A23L5/32A23L5/40A23L33/17A23V2250/542A23V2300/30A23V2300/48A23V2300/38A23V2300/14A23V2200/046
Inventor 张立新周小进胡小明潘世俊石继均梅德祥盛家镛
Owner NANTONG LOVER APPL
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