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Method for measuring cefadroxil concentration in plasma through hygroplasm combination

A technique of cefadroxil and liquid mass spectrometry, which is applied in the field of medicine, can solve the problems of cefadroxil determination methods, etc., and achieve the effect of simple pretreatment method, good peak shape and stable baseline

Inactive Publication Date: 2019-04-26
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report on the determination method of cefadroxil

Method used

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  • Method for measuring cefadroxil concentration in plasma through hygroplasm combination
  • Method for measuring cefadroxil concentration in plasma through hygroplasm combination
  • Method for measuring cefadroxil concentration in plasma through hygroplasm combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] Example: Human K 2 Determination of Cefadroxil Concentration in EDTA Plasma

[0035] 1. Experimental materials and analytical equipment

[0036] Cefadroxil (analyte): Toronto Research Chemicals or equivalent or higher grade standard

[0037] Cefadroxil-d4 (internal standard): Toronto Research Chemicals or the same or higher grade standard

[0038] The reagents used are shown in Table 1 below:

[0039] Table 1 Reagent details

[0040] Reagent name

level

manufacturer

Acetonitrile

HPLC

J.T.Baker

Methanol

HPLC

J.T.Baker

Formic acid

ACS

Adamas

[0041] Note: Reagents of the same grade or higher can also be used

[0042] The analysis equipment used is shown in Table 2 below:

[0043] Table 2 Details of equipment used

[0044]

[0045]

[0046] The same LC / MS / MS system can also be used.

[0047] 2. Liquid quality conditions

[0048] 1. Liquid chromatography conditions

[0049] Chromatographic column: RestekAllure PFPP, 5um, column specification 50×2.1mm; Chromatographic colu...

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Abstract

The invention discloses a method for measuring cefadroxil concentration in plasma through hygroplasm combination. A hygroplasm combination system is adopted for measurement. The method comprises the steps that firstly, a to-be-measured sample is taken, a certain quantity of mixed organic solvent is added for conducting extraction twice, and after pretreatment, through chromatographic column separation, a mass spectrometry detector is used for detection. The method is quick to use, accurate, high in sensitivity and easy and convenient to operate, and the basis is provided for plasma concentration measurement of cefadroxil. The linear range of a plasma standard curve is within 0.1-60 mug / mL, the within-run precision and between-run precision RSD are smaller than + / -15%, and the method is suitable for measurement of cefadroxil concentration in plasma.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a method for determining a drug, in particular to a method for determining the concentration of cefadroxil in plasma by liquid-mass spectrometry. Background technique [0002] Cefadroxil (Cefadroxil, CFD), chemical name: (6R, 7R)-3-methyl-7-[(R)-2-amino-2-(4-hydroxyphenyl)acetamido]-8- Oxy-5-thia-1-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid monohydrate, molecular formula: C 16 H 17 N 3 O 5 S·H 2 O, molecular weight: 381.41, structural formula: [0003] [0004] Cefadroxil is a bactericidal cephalosporin broad-spectrum antibiotic, the first-generation semi-synthetic cephalosporin drug of Bristol-Meier, USA, which can cause infections of gram-positive and gram-negative bacteria effective. Oral cefadroxil preparations can be used to treat mild to moderately susceptible infections, such as pharyngitis or streptococcal tonsillitis caused by the bacteria Streptococcus pyogenes, u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/34
CPCG01N30/34G01N30/88G01N2030/045G01N2030/342G01N2030/8822
Inventor 黄靖智
Owner 徐州立兴佳正医药科技有限公司
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