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Method for extracting cyclic adenosine monophosphate and cyclic guanosine monophosphate from jujube fruits

A technology of cyclic guanosine monophosphate and cyclic adenosine monophosphate, which is applied in the field of preparation of cyclic guanosine monophosphate and cyclic guanosine monophosphate in jujube fruit, can solve the problems of poor extraction efficiency and low purity, and achieve long service life, high purity, The effect of targeted separation

Inactive Publication Date: 2019-04-19
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology for extracting cAMP from jujube also has the problems of poor extraction efficiency and low purity, and there are few reports on the process of extracting cyclic guanosine monophosphate from jujube.

Method used

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  • Method for extracting cyclic adenosine monophosphate and cyclic guanosine monophosphate from jujube fruits
  • Method for extracting cyclic adenosine monophosphate and cyclic guanosine monophosphate from jujube fruits
  • Method for extracting cyclic adenosine monophosphate and cyclic guanosine monophosphate from jujube fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 A preparation method of cyclic adenosine monophosphate and cyclic guanosine monophosphate in jujube fruit

[0040] (1) Preparation of jujube crude extract

[0041] The jujube fruit is depitted and pulverized to obtain jujube powder, and the jujube powder is added to the extraction solvent at a ratio of 1:10 by weight and volume of the jujube powder and the extraction solvent, and heated and refluxed for extraction. The extraction solvent is 15% ethanol aqueous solution, and the extraction temperature is 90°C, extract for 2 hours each time, repeat the extraction twice, combine the extracts, concentrate under reduced pressure, recover ethanol, and obtain the crude jujube extract;

[0042] (2) Alcohol precipitation

[0043] Add the jujube crude extract in step (1) into 75% ethanol aqueous solution, let stand at 4°C for 24 h, filter, and concentrate the supernatant under reduced pressure to obtain crude extracts of cyclic adenosine monophosphate and cyclic guanos...

Embodiment 2～11

[0050] The preparation method of cyclic adenosine monophosphate and cyclic guanosine monophosphate in jujube fruit of embodiment 2-11

[0051] Examples 2 to 11 are respectively a preparation method of cyclic adenosine monophosphate and cyclic guanosine monophosphate in jujube fruit. The operation steps are the same as in Example 1, except for the type and purity of the alcohol, and the reaction time in the process, The control parameters such as reaction temperature are different, see the following table for details:

[0052]

[0053]

Embodiment 12

[0054] Example 12 Qualitative experiment of cyclic adenosine monophosphate and cyclic guanosine monophosphate

[0055] Three methods were used to jointly characterize the cyclic adenosine monophosphate and cyclic guanosine monophosphate prepared in any one of Examples 1-11.

[0056] Method 1: Qualitative analysis by HPLC retention time and UV absorption spectrum

[0057] Detection instrument: high performance liquid chromatography equipped with a diode array detector.

[0058] Detection conditions:

[0059] Chromatographic column: C18 chromatographic column (4.6×250 mm, 5 µm);

[0060] Mobile phase: methanol + 0.1% formic acid aqueous solution (20:80);

[0061] Flow rate: 1.0 mL / min;

[0062] Detection wavelength: 255 nm;

[0063] Wavelength scanning range: 210 nm-400 nm.

[0064] Under the same chromatographic conditions, the retention time and ultraviolet absorption spectrum of the prepared cyclic adenosine monophosphate and cyclic guanosine monophosphate were compared w...

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Abstract

The invention discloses a method for extracting cyclic adenosine monophosphate and cyclic guanosine monophosphate from jujube fruits. The method comprises the steps of preparation of crude jujube extracts, alcohol precipitation, macroporous resin chromatographic column separation, membrane separation and preparative liquid chromatography separation. In the method, alcohol is used as an extractingsolution, the three separation ways including macroporous resin chromatographic column separation, membrane separation and preparative liquid chromatography separation are adopted, the cyclic adenosine monophosphate and the cyclic guanosine monophosphate which are obtained by using the method are high in purity, and the purity of the cyclic adenosine monophosphate and the purity of the cyclic guanosine monophosphate reach 90% or above respectively. When the preparative liquid chromatography is applied, on-line monitoring can be achieved, target separation is achieved, and the separation efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to the extraction of natural components in jujube fruits, in particular to a preparation method of cyclic adenosine monophosphate and cyclic guanosine monophosphate in jujube fruits. Background technique [0002] Cyclic guanosine monophosphate and cyclic adenosine monophosphate are both second messengers with intracellular information transmission. Cyclic adenosine monophosphate is produced by adenosine triphosphate under the catalysis of adenylyl cyclase. It can regulate various functional activities of cells, and plays an important role in the regulation of sugar and fat metabolism, nucleic acid and protein synthesis. Cyclic guanosine monophosphate has the effect of acetylcholine, inhibits myocardial contractility, lowers heart rate, increases nerve excitability, stimulates leukocyte lysosomes to release hydrolytic enzymes, stimulates lymphocyte division and proli...

Claims

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Application Information

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IPC IPC(8): C07H19/213C07H1/08
CPCC07H1/08C07H19/213
Inventor 李存满罗晓田晶
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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