Method for extracting phaseolin polypeptide from asparagus

A protein peptide, asparagus technology, applied in the direction of fermentation, to achieve the effects of high extraction efficiency, inhibition of cell degeneration, and mild reaction conditions

Inactive Publication Date: 2019-04-16
山东巨鑫源农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report of extracting protein polypeptide from asparagus at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting protein polypeptides from asparagus of the present invention comprises the following steps:

[0024] (1) Raw material pretreatment

[0025] Fresh asparagus is washed to remove impurities, dried, put into a pulverizer and pulverized for 10-15 minutes to obtain asparagus powder.

[0026] (2) Alkali solubility

[0027] Add pure water to the obtained asparagus powder to form a slurry. The mass ratio of asparagus powder to purified water is 1:17, and a stabilizer is added. The stabilizer is fructan, and the quality of the added stabilizer is 5% of the mass of asparagus powder. ‰, adjust the pH value to 8 with 0.1mol / L acetic acid solution, then heat to 53°C, keep it warm for 0.8h, then filter, and take the filtrate to obtain protein solution.

[0028] (3) One-time enzymatic hydrolysis

[0029] Lower the protein solution to normal temperature, adjust the pH value to 8, add alkaline protease to the protein solution to obtain the first mixed solution,...

Embodiment 2

[0037] (1) Raw material pretreatment

[0038] Fresh asparagus is washed to remove impurities, dried, put into a pulverizer and pulverized for 10-15 minutes to obtain asparagus powder.

[0039] (2) Alkali solubility

[0040] Add pure water to the obtained asparagus powder to form a slurry. The mass ratio of asparagus powder to purified water is 1:23, and a stabilizer is added. The stabilizer is fructan, and the quality of the added stabilizer is 2% of the mass of asparagus powder. ‰, adjust the pH value to 9 with 0.1mol / L acetic acid solution, then heat to 55°C, keep it warm for 1h, then filter, and take the filtrate to obtain protein solution.

[0041] (3) One-time enzymatic hydrolysis

[0042] Lower the protein solution to normal temperature, adjust the pH value to 7, add alkaline protease to the protein solution to obtain the first mixed solution, then stir evenly, heat again for 15 minutes, control the temperature at 58°C, and adjust to maintain the first mixed solution. ...

Embodiment 3

[0050] (1) Raw material pretreatment

[0051] Fresh asparagus is washed to remove impurities, dried, put into a pulverizer and pulverized for 10-15 minutes to obtain asparagus powder.

[0052] (2) Alkali soluble

[0053] Add pure water to the obtained asparagus powder to form a slurry. The mass ratio of asparagus powder to purified water is 1:20, and a stabilizer is added. The stabilizer is fructan, and the quality of the added stabilizer is 4% of the mass of asparagus powder. ‰, adjust the pH value to 9 with 0.1mol / L sodium carbonate solution, then heat to 57°C, keep it warm for 1.2h, then filter, and take the filtrate to obtain protein solution.

[0054] (3) One-time enzymatic hydrolysis

[0055] Lower the protein solution to normal temperature, adjust the pH value to 8, add alkaline protease to the protein solution to obtain the first mixed solution, then stir evenly, heat again for 17 minutes, control the temperature at 59°C, and adjust to maintain the first mixed soluti...

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PUM

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Abstract

The invention belongs to the field of asparagus food deep processing, and particularly relates to a method for extracting phaseolin polypeptide from asparagus. The invention discloses the method for extracting phaseolin polypeptide from asparagus for the first time. Fresh asparagus is made into asparagus powder, then, alkali dissolution and enzymolysis are performed to obtain phaseolin polypeptide, and finally concentration is performed to obtain asparagus phaseolin polypeptide. According to the method for extracting phaseolin polypeptide from asparagus facilitating commercial production, theextracted asparagus phaseolin polypeptide is easily absorbed by the human body, cell denaturation, detoxification and virus resistance can be restrained, and the phaseolin polypeptide can be widely applied to the fields of cosmetics, healthcare food and medicine products, and is suitable for being widely popularized.

Description

technical field [0001] The invention belongs to the field of deep processing of asparagus food, in particular to a method for extracting protein polypeptide from asparagus. Background technique [0002] Asparagus contains protein, trace elements and various amino acids necessary for the human body. Asparagus protein peptides can be used as raw materials for functional food, pharmaceutical products, and health foods. Many studies have shown that there are a large number of biologically active components in asparagus protein peptides, including easy absorption by the human body, improving immunity, activating cells to resist infection, anti-virus and other biological activities of polypeptides. The protein polypeptides in asparagus exist in the form of peptides and free amino acids, which are easy to absorb by the human body, can inhibit cell degeneration, enhance human immunity, activate cells, remove harmful free radicals and heavy metal compounds, perform effective detoxif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 周长生孙德林刘永孙研孙辉崔云前陆丕聪
Owner 山东巨鑫源农业科技有限公司
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