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Measuring method for GCMS fingerprint spectrum of Xingnaojing injection and standard fingerprint spectrum thereof

A technology for Xingnaojing injection and a determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not simultaneously measuring the four medicinal materials in Xingnaojing injection, and achieve the perfection of quality control technology and guarantee The effect of high accuracy and similarity

Active Publication Date: 2019-04-12
WUXI JIYU SHANHE PHARM CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the simultaneous determination of the components of the four medicinal materials in Xingnaojing injection

Method used

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  • Measuring method for GCMS fingerprint spectrum of Xingnaojing injection and standard fingerprint spectrum thereof
  • Measuring method for GCMS fingerprint spectrum of Xingnaojing injection and standard fingerprint spectrum thereof
  • Measuring method for GCMS fingerprint spectrum of Xingnaojing injection and standard fingerprint spectrum thereof

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Experimental program
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Effect test

Embodiment 1

[0046] 1. Instruments and reagents

[0047] 1.1 Instrument

[0048]One hundred thousandth analytical balance (Sartorious), Agilent Intuvo 9000 / 5977B GC-MS, Milli-Q deionized water system, high-speed refrigerated centrifuge.

[0049] 1.2 Reagents

[0050] Camphor, borneol, β-elemene, curcumone, protocurcumenol, gemaconone, curedione, curcumenone, muscone reference substance, methanol, mass spectrometry water.

[0051] 2. Preparation of the test article

[0052] Preparation of the test solution: Precisely pipette 10 mL of Xinnaojing injection, pass it through a C18 solid-phase extraction column (activated with 3 ml of methanol in advance, equilibrated with 3 ml of water); rinse with 3 ml of mass spectrometry water; divide into two times, each time with 2 ml of methanol Clean the filler, collect the eluate in a 5ml centrifuge tube, add excess anhydrous sodium sulfate to remove excess water, transfer to a 5ml volumetric flask, wash anhydrous sodium sulfate with a small amount o...

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Abstract

The invention relates to a measuring method for a GCMS fingerprint spectrum of a Xingnaojing injection and a standard fingerprint spectrum thereof. The measuring method comprises the following steps:(1) preparation of a test solution: precisely transferring 5-10mL of the Xingnaojing injection, passing through a C18 solid phase extraction cartridge; collecting an eluate into a 1-5ml volumetric flask, adding an organic solvent to a scale line, and obtaining the test solution; (2) preparation of a reference solution: weighing reference substances of camphor, borneol, beta-elemene, curzerenone, germacrone, curdione, curcumenone, and musk ketone and adding into the organic solvent to prepare a solution having a known concentration, and obtaining the reference solution; and (3) the measuring method: injecting the reference solution and the test solution into a gas chromatograph-mass spectrometer, measuring, and recording a chromatography spectrum.

Description

technical field [0001] The invention relates to a method for analyzing fingerprints of traditional Chinese medicines, in particular to a method for determining the temperament fingerprint of Xingnaojing injection, and the obtained temperament fingerprint of Xingnaojing injection. technical background [0002] Xingnaojing Injection began in the mid-1970s as a flavor-reduced prescription of "Angong Niuhuang Pills" ("Wenbing Tiaobian", Wu Jutong in Qing Dynasty), consisting of musk (7.5g), turmeric (30g) , borneol (1g), gardenia (30g). The excipients are polysorbate 80 and sodium chloride. It has the effects of clearing heat and detoxifying, cooling blood and detoxifying, resuscitating the brain, and is used for stroke coma caused by qi and blood stasis, brain stasis, hemiplegia and oral cavity; traumatic headache, coma; alcohol poisoning attacking the heart, headache, nausea, coma and convulsions , Cerebral embolism, acute cerebral hemorrhage, craniocerebral trauma, acute al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74
CPCG01N30/74
Inventor 杨华李萍范君丽
Owner WUXI JIYU SHANHE PHARM CO LTD
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