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Nano gold-protoporphyrin zinc (ii) highly sensitive detection h 2 o 2 Construction and Application of Electrochemical Sensors

A protoporphyrin zinc, H2O2 technology, applied in the fields of material electrochemical variables, scientific instruments, nanotechnology, etc., can solve the problems of long analysis time, high detection cost, low detection limit, etc., and achieves good chemical stability, biological The effect of good compatibility and low detection limit

Active Publication Date: 2021-04-27
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods have disadvantages such as high detection cost, complicated operation, long analysis time, and low detection limit.

Method used

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  • Nano gold-protoporphyrin zinc (ii) highly sensitive detection h <sub>2</sub> o <sub>2</sub> Construction and Application of Electrochemical Sensors
  • Nano gold-protoporphyrin zinc (ii) highly sensitive detection h <sub>2</sub> o <sub>2</sub> Construction and Application of Electrochemical Sensors
  • Nano gold-protoporphyrin zinc (ii) highly sensitive detection h <sub>2</sub> o <sub>2</sub> Construction and Application of Electrochemical Sensors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Synthesis of nano-gold-protoporphyrin zinc (II) (PEI-AuNPs-ZnPP) biomimetic enzyme:

[0048] (1) Weigh 2.06g of polyethyleneimine (PEI) and 340μL of 2% chloroauric acid (HAuCl 4 ) solution, then add 1960mL ultrapure water, heat up to 80°C intermittently while stirring, keep it warm, observe the color change of the solution, when the solution turns light ruby ​​red, stop heating, continue stirring to room temperature, and obtain nano-gold (PEI-AuNPs) solution, stored at 4°C in the dark for future use;

[0049] (2) dissolving zinc protoporphyrin (II) (ZnPP) in N,N-dimethylformamide (DMF) to prepare a ZnPP solution with a concentration of 0.1 mmol / L;

[0050] (3) Mix 4.8mL ZnPP solution with 4mL nano-gold solution, and stir the mixture on a magnetic stirrer for 1h;

[0051] (4) After stirring, transfer the mixture to a centrifuge tube, and centrifuge at 14000 rpm for 80 min at 4°C;

[0052] (5) After centrifugation, discard the supernatant, then add 1.0 mL...

Embodiment 2

[0065] Embodiment 2: the preparation of electrochemical sensor

[0066] (1) Electrode (GCE) cleaning:

[0067] On the polishing plate, use alumina polishing powder with a particle size of 0.3 μm to polish the glassy carbon electrode to the mirror surface, and then insert the glassy carbon electrode into 5 mmol / L potassium ferricyanide solution for linear cyclic voltammetry scanning test (scanning range: - 0.2V~0.6V, scan rate: 0.05V / s, sampling interval 1mV; static time: 5s, cathode current is positive, when the redox peak potential difference of the glassy carbon electrode should be less than 80mV, the electrode treatment is considered qualified. If not , then repeat the above operation). Put the qualified glassy carbon electrode into an ultrasonic cleaner, wash it with ultrapure water, absolute ethanol, and ultrapure water for 1 min, and finally dry it with nitrogen.

[0068] (2) Electrode modification:

[0069] Add 4-6 μL of nano-gold-protoporphyrin zinc (II) dropwise on...

Embodiment 3

[0070] Embodiment 3: Feasibility detection of electrochemical sensor

[0071] 5 μL of gold nanoparticles (PEI-AuNPs), zinc protoporphyrin (II) (ZnPP), gold nanoparticles-zinc protoporphyrin (II) (PEI-AuNPs-ZnPP) were respectively modified on the surface of the electrode (the method was the same as in Example 2 ), and the bare electrode, vacuum-dry for 30min, add 10μL 0.5mol / L H 2 o 2 , measured by differential pulse voltammetry (scanning range: 0V ~ -1V), the results are as follows Figure 4 , the current signal of nano-gold-protoporphyrin zinc (Ⅱ) is the strongest, which proves that the method is feasible.

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Abstract

The invention discloses nano gold-protoporphyrin zinc (II) highly sensitive detection of H 2 o 2 The construction and application of electrochemical sensors, specifically related to the synthesis of a nano-gold-protoporphyrin zinc (Ⅱ) (PEI-AuNPs-ZnPP) and its effect on H in human serum 2 o 2 detection. The present invention utilizes the feature that nano-gold and protoporphyrin zinc can be connected by amide bond to synthesize, modify it on the surface of glassy carbon electrode, and detect H by electrochemical method. 2 o 2 Concentration, the results show that under optimal conditions, within a certain range of H 2 o 2 The concentration and the current signal are positively correlated, the linear equation is I(μA)=0.20412c(pmol / L)+1.93557, the correlation coefficient is 0.99534, and the detection limit is 8.540×10 ‑13 M. Its stability is better in that the current signal of the sensor is 96.8% of the initial value after 3 days, and at the same time, the sensor can measure H in the actual sample human serum 2 o 2 Also showed a high recovery rate (97.57 ~ 101.43%), can be used to detect H 2 o 2 The concentration of glucose laid the foundation for the later application in the medical field to detect the concentration of glucose.

Description

technical field [0001] The invention belongs to the field of construction and application of electrochemical sensors, in particular to the synthesis of a nano-gold-protoporphyrin zinc (II) (PEI-AuNPs-ZnPP) and its ability to react to H in human serum. 2 o 2 The detection belongs to the technical field of bioanalysis. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) as an important biomarker, widely used in food production, environmental monitoring, drug synthesis and clinical testing and other fields, hydrogen peroxide is a toxic by-product produced in the process of human metabolism, which can cause great damage to the body, the study found h 2 o 2 Overdose can cause Alzheimer's, Parkinson's and other central nervous system disorders; therefore H 2 o 2 Sensitive detection of content is of great significance in the fields of physiology, pathology and environment. At present, there are many methods for the determination of hydrogen peroxide content, such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/416G01N27/48B82Y30/00
CPCB82Y30/00G01N27/308G01N27/4163G01N27/48
Inventor 杨怀霞刘艳菊李晓飞李金鸽李曼曼郭文峰赵可欣
Owner HENAN UNIV OF CHINESE MEDICINE
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