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Micromanipulation dish and sperm selection method for preferred sperms

A technology of micromanipulation and sperm, which is applied in the field of assisted reproduction, can solve the problems affecting the fertilization process of sperm eggs and embryo development, the limited ability to select high-quality sperm, and increase the inheritance of offspring of patients, etc., and achieve great practical value and application prospects. Strong, low-cost effect

Pending Publication Date: 2019-04-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of sperm screening methods, operators can only select sperm empirically based on sperm motility and surface morphology, and the ability to select high-quality sperm is very limited. The so-called "normal" sperm selected may contain certain physiological function defects. This will affect the fertilization process of sperm and eggs and embryo development, which may increase the risk of genetic defects in the offspring of patients receiving treatment, and may even lead to the failure of ICSI outcome

Method used

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  • Micromanipulation dish and sperm selection method for preferred sperms
  • Micromanipulation dish and sperm selection method for preferred sperms
  • Micromanipulation dish and sperm selection method for preferred sperms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this embodiment, a micromanipulation dish for preferred sperm is made of a transparent, hard, high temperature-resistant, corrosion-resistant, non-toxic and non-pyrogenic polymer material. Such as figure 1 As shown, the micromanipulation dish includes: a dish plate and a dish cover, and the upper surface of the dish plate is concavely provided with a sample pool 1, a first channel 21, and a second channel 22, and the sample 1 passes through the middle of the first channel 21 and the second channel 22. point connection, two induction chambers 3 are respectively arranged on both sides of the midpoint of the second channel 22, and the upper surface of the dish is also divided into a sperm collection area 4, a sperm braking area 5 and a microinjection area 6, and the second channel 22 The two ends of the tube are respectively connected to the sperm collection area 4 through an upwardly inclined third channel 23, and the sperm braking area 5 and the microinjection area 6 ...

Embodiment 2

[0056] The experimental group used the preferred sperm micromanipulation dish of the present invention, and the test preparation was the same as in the first part of Example 1.

[0057] Take an appropriate amount of sperm specimens and slowly and gently add them to the sample pool 1, cover the dish, and put it in the carbon dioxide incubator for 30-60 minutes. The sperm with normal physiological functions and fertilization ability will swim to the sperm collection area 4. Sperm in sperm collection area 4 are immediately available for intracytoplasmic sperm microinjection. That is, use a micropuncture needle to draw a sperm in the sperm collection area 4, move to the sperm braking area 5 to immobilize the sperm, and then move to the microinjection area 6. Fix the egg in the microinjection area 6 with a microfixation needle, make its polar body at the 6 o'clock or 12 o'clock position, and inject the sperm into the egg to complete in vitro fertilization. In the experimental grou...

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Abstract

The invention discloses a micromanipulation dish and a sperm selection method for preferred sperms. The micromanipulation dish for the preferred sperms comprises a dish pan and a dish cover; concave sample pool and selection passageways are arranged on an upper surface of the dish pan, wherein the selection passageways comprise a first passageway, a second passageway and third passageways; the sample pool is connected with a midpoint of the second passageway via the first passageway; a plurality of induction chambers are separately arranged on the two sides of the midpoint of the second passageway; the upper surface of the dish pan is further divided into a sperm collection area, a sperm braking area and a micro-injection area; two ends of the second passageway are respectively connected with the sperm collection area via the third passageways which slope upwards; and the sperm braking area and the micro-injection area are arranged close to the micro-injection area. Being utilized, themicromanipulation dish for the preferred sperms is capable of simulating motion trajectory of sperm in female genital tracts, as well as adsorption of apoptotic sperms by connexin V and regulation ofprogesterone on sperm functions, so that, selected preferred sperms can be immediately used for intracytoplasmic sperm microinjection, thereby beneficially improving fertilization rate of in vitro fertilization.

Description

technical field [0001] The invention belongs to the technical field of assisted reproduction, and in particular relates to a micromanipulation dish for optimizing sperm and a method for optimizing sperm. Background technique [0002] During the natural fertilization process of human beings, mature sperm enter the female reproductive tract and need to undergo physiological processes such as capacitation, hyperactivation, and acrosome reaction, and finally combine with egg cells to complete fertilization. Progesterone is distributed in a concentration gradient in the female reproductive tract. When sperm enter the reproductive tract, low concentration of progesterone can quickly induce the increase of calcium ion concentration in sperm cells and induce sperm capacitation. When the sperm meets the egg cell, the high concentration of progesterone can induce the acrosome reaction of the sperm, so that the sperm and egg fuse and fertilization occurs. The concentration gradient d...

Claims

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Application Information

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IPC IPC(8): C12M1/22C12M1/00C12N5/076
CPCC12M23/10C12N5/061
Inventor 薛亚梅李坤张松英
Owner ZHEJIANG UNIV
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