Method for cultivating salt stress-resistant lactococcus lactis and special culture medium therefor
A technology of Lactococcus lactis and culture medium, which is applied in the field of cultivating salt-stress-resistant Lactococcus lactis, can solve problems such as death and decreased strain vitality, and achieve the effects of increasing survival rate, increasing survival rate, and enhancing added value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0013] Embodiment 1, cultivating Lactococcus lactis resistant to salt stress
[0014] a) culture medium:
[0015] LM17 medium: Take 5.0g of phytopeptone, 5.0g of polypeptone, 2.5g of beef extract, 15g of agar, 19g of β-glycerophosphate disodium, 5.0g of yeast extract, 0.5g of ascorbic acid, MgSO4 7H 2 O 0.25g, dissolved in 1L of distilled water to make the pH 7.1, sterilized at 121°C for 15min, and then added 15g of lactose per liter.
[0016] Total synthetic medium (CDM) (g L -1 ):
[0017] (1) take K 2 HPO 4 3g, 1g sodium acetate (pre-dissolved in 400μl 10M NaOH), 0.6g triammonium citrate, 0.5g tyrosine;
[0018] (2) Pre-dissolve 0.5g vitamin C in 5ml distilled water;
[0019] (3) Mix (1) and (2), then add 19g of β-glycerophosphate disodium, distill the volume to 870ml with distilled water, then add 100ml of 10× amino acid concentrate, 10ml of metal ion concentrate, and 10ml of nucleotide Concentrate, 10ml vitamin concentrate, adjust the pH to 6.8 with 5M NaOH, and fi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com