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Astragalus membranaceus seed-dressing agent and method for promoting growth of astragalus membranaceus and accumulation of astragalus root flavonoids compounds of astragalus membranaceus seed-dressing agent

A seed dressing agent and technology of Astragalus membranaceus, applied in the field of Astragalus membranaceus seed dressing agent and its promotion of Astragalus membranaceus growth and the accumulation of flavonoids in Astragalus root, to achieve the effects of increasing yield and quality, improving the quality and yield of medicinal materials, and facilitating continuous development and utilization

Active Publication Date: 2019-04-05
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the current problems of astragalus cultivation and quality improvement, the present invention provides an astragalus seed dressing agent and a method for promoting the growth of astragalus and the accumulation of flavonoids in astragalus roots, so as to improve the quality and yield of astragalus under drought stress conditions

Method used

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  • Astragalus membranaceus seed-dressing agent and method for promoting growth of astragalus membranaceus and accumulation of astragalus root flavonoids compounds of astragalus membranaceus seed-dressing agent
  • Astragalus membranaceus seed-dressing agent and method for promoting growth of astragalus membranaceus and accumulation of astragalus root flavonoids compounds of astragalus membranaceus seed-dressing agent
  • Astragalus membranaceus seed-dressing agent and method for promoting growth of astragalus membranaceus and accumulation of astragalus root flavonoids compounds of astragalus membranaceus seed-dressing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Isolation of Pseudomonas phlei KSC02, an endophyte of Astragalus membranaceus, and screening of growth-promoting characteristics

[0018] The 5-year-old fresh roots of Astragalus membranaceus in Hunyuan, Shanxi Province were used as materials, and the endophytic bacteria of Astragalus membranaceus were isolated by tissue block separation method. The specific operation is as follows: wash the harvested fresh Astragalus membranaceus several times with tap water, wash away the soil remaining on the root surface, and then rinse it with sterile water containing 10% Tween-20; Cut into 1cm-long sections, soak in 75% alcohol for 1 min, rinse with sterile water for 3 times, soak in 5% sodium hypochlorite for 10 min, rinse with sterile water for 3 times, and then rinse with 75% alcohol for 40 seconds. Rinse with sterile water for 5 times, absorb excess water with sterile filter paper, put the treated material in a sterile mortar, add 2 mL of normal saline to mash, and ...

Embodiment 2

[0027] Embodiment 2: the impact of environmental stress on the growth of Pseudomonas phlei KSC02

[0028] Pick the isolated, purified, identified and stored Pseudomonas phlegm strains at -80°C, first streak on a Luria-Bertani (LB) solid plate, and culture at 28°C, take a single colony and continue to streak culture once, to Activated strains. Pick a single colony of the second activated strain, transfer it to LB liquid medium, and culture it on a shaker at 28°C until late logarithmic growth (OD600 between 1.8 and 2.0) to obtain a seed culture. Inoculate the above culture in LB liquid medium containing 1%, 2%, 3.5%, 5.0% and 7% NaCl according to the inoculum amount of 1‰, and use the one without NaCl as the negative control, and do three parallels for each concentration, shake The bed was cultured overnight at 28°C, with a rotation speed of 180rpm, and a part of the culture was taken for OD600 measurement directly to obtain the growth data of the bacteria. Taking NaCl concent...

Embodiment 3

[0031] Example 3: Growth (a) and IAA production (b) characteristics of Pseudomonas phrysalis KSC02 under extreme stress conditions

[0032] The seed culture was prepared by the method of Example 2. According to the inoculation amount of 1‰, it was inoculated in the LB liquid medium containing 7% NaCl, 20% PEG6000 and pH 5.0 with 1 mg / mL tryptophan added, and the normal LB medium containing 1% NaCl at pH 7.2 was As a control, cultured on a shaker at 28°C, with a rotation speed of 180rpm. OD600 and OD530 were measured at 5, 9, 13, 17, and 30 h of culture, respectively. Among them, part of the culture was directly used to measure OD600; at the same time, 1.5 mL of culture solution was mixed with an equal volume of Salkowski chromogen (10 ml of 0.5 mol / L FeCl 3 , 500 ml of 35% perchloric acid, mixed before use, stored away from light) fully mixed, placed in the dark at room temperature for 30 min, and measured OD530; with indole acetic acid as the reference substance, the workin...

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Abstract

The invention belongs to the technical field of ecological planting of astragalus membranaceus, aims to solve the problems about cultivation of the astragalus membranaceus and improvement of quality,and provides an astragalus membranaceus seed-dressing agent and a method for promoting growth of astragalus membranaceus and accumulation of astragalus root flavonoids compounds of the astragalus membranaceus seed-dressing agent. The astragalus membranaceus seed-dressing agent is prepared by fermenting a Pseudomonas poae strain KSC02. The specific preparation method comprises the steps of preparing the strain KSC02 into a seed culture, connecting the seed culture into an LB liquid medium, carrying out shake cultivation at the temperature of 28 DEG C and at the speed of 180 rpm for overnight, centrifuging the culture, and collecting strain precipitates; and washing the strain precipitates twice with 300 mM MgCl2, then settling a suspended strain in a MgCl2 solution, adjusting OD600 to be 1.0 to prepare the seed-dressing agent, and storing the seed-dressing agent at the temperature of 4 DEG C for subsequent use. The yield and quality of the astragalus membranaceus under a stress condition are improved, the astragalus membranaceus seed-dressing agent is expected to be developed into green pesticide, and double effects of improving the quality and yield of medicinal materials and reducing chemically synthesized pesticide and fertilizers are achieved.

Description

technical field [0001] The invention belongs to the technical field of astragalus ecological planting, in particular to an astragalus seed dressing agent and a method for promoting the growth of astragalus and the accumulation of flavonoids in astragalus root. Pseudomonas poae Seed dressing of strain KSC02 promoted the increase of root biomass and root-to-shoot ratio of Astragalus membranaceus under drought stress conditions, as well as the content of flavonoid active components formononetin and acteosin glucoside in roots of medicinal parts. Background technique [0002] Existing studies have shown that endophytes synthesize plant growth hormone indoleacetic acid (IAA) and use ethylene in plants to synthesize precursors—1-aminocyclopropane-1-carboxylic acid (1-aminocyclopropane-1-carboxylic acid). , ACC), as a carbon source, plays an active role in plant growth, synthesis and accumulation of secondary metabolites, and mitigation of environmental stress. For the role of end...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00A01P3/00A01P1/00
CPCA01N63/30
Inventor 孙海峰高红柴智孔留利曹秋芬高建平开国银郭兰萍
Owner SHANXI UNIV
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