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A kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and preparation method thereof

A polyamino acid and nanogel technology, applied in the field of biomedicine, can solve the problems of low photosensitizer transfer efficiency, poor protein escape ability, low protein loading efficiency, etc., to promote cell uptake, improve cancer treatment efficiency, good biophase Effects of Capacitance and Biodegradability

Active Publication Date: 2021-09-24
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and its preparation method in order to overcome the defects existing in the prior art. , poor protein escape ability, low photosensitizer transfer efficiency, poor diagnostic sensitivity, etc., and realize the function of simultaneous treatment of proteins and photosensitizers

Method used

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  • A kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and preparation method thereof
  • A kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and preparation method thereof
  • A kind of protein and photosensitizer co-delivery pH-responsive polyamino acid nanogel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0039] (1) During the preparation process, 10g of L-glutamic acid γ-benzyl ester and 6.3g of triphosgene were placed in a 250mL three-necked flask with a magnetic rotor, and were poured into 100mL of anhydrous tetrahydrofuran to dissolve it completely, and the reaction The mixture was reacted in a 60°C oil bath for 1.5 hours under nitrogen protection. Subsequently, the reaction solution was dropped into 1L of frozen n-hexane, and then put into a -20 degree refrigerator for recrystallization overnight. The next day, the mixed solution was filtered and recrystallized twice with a mixed solution of ethyl acetate and n-hexane (1:2 by volume) at -20°C. Finally, the solution was filtered and the remaining solid was vacuum dried to obtain pure BLG-NCA monomer.

[0040] (2) 1g single-ended polyethylene glycol monomethyl ether (mPEG-NH 2 , the average molecular weight is 5000) and 2.7g BLG-NCA were dissolved in 10mL of anhydrous chloroform, and the reaction solution was reacted at ro...

Embodiment 2

[0046] (1) During the preparation process, 10g of L-aspartate α-benzyl ester and 6.5g of triphosgene were placed in a 250mL three-necked flask with a magnetic rotor, and were poured into 100mL of anhydrous tetrahydrofuran to dissolve it completely, and the reaction The material was reacted for 1.5 hours in a 55 degree oil bath under the condition of nitrogen protection. Subsequently, the reaction solution was dropped into 1L of frozen n-hexane, and then put into a -20 degree refrigerator for recrystallization overnight. The next day, the mixed solution was filtered and recrystallized twice with a mixed solution of ethyl acetate and n-hexane (1:3 by volume) at -20°C. Finally, the solution was filtered and the remaining solid was vacuum dried to obtain pure BLA-NCA monomer.

[0047] (2) 1g single-ended polyethylene glycol monomethyl ether (mPEG-NH 2 , the average molecular weight is 5000) and 2.7g BLA-NCA were dissolved in 10mL of anhydrous chloroform, and the reaction solutio...

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Abstract

A protein and a photosensitizer co-deliver pH-responsive polyamino acid nanogel and its preparation method, belonging to the technical field of biomedicine. The pH-responsive nanogel prepared by the present invention is to initiate γ-benzyl-L-glutamic acid ester-N-endocarboxylate in one step by using polyethylene glycol monomethyl ether with an amine group as a macroinitiator. anhydride or γ‑benzyl‑L‑aspartic acid ester‑N‑internal carboxylic acid anhydride ring-opening polymerization to prepare the polyamino acid backbone, and then the tail of the backbone is used to graft photosensitizers, while the side chains are grafted with cationic molecules Modifications to increase protein loading capacity and lysosomal escape capacity. Finally, a pH-responsive nanogel system is constructed through the inner core cross-linking reaction, so as to achieve the purpose of safe delivery, targeted delivery, rapid cell uptake in vivo, release of proteins and activation of photosensitizers in vivo. This will help promote the development of combined protein and photodynamic therapy in the medical field.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a pH-responsive polyamino acid nanogel co-delivered by a protein and a photosensitizer and a preparation method thereof. Background technique [0002] Cancer has become one of the serious diseases that threaten human health and has the highest mortality rate, and shows an increasing trend year by year. According to the latest data released by the National Cancer Center in 2017, about 10,000 people are diagnosed with cancer every day in the country, and the mortality rate is as high as 60%, and cancer patients are gradually becoming younger. Therefore, it is particularly important for human development, family happiness and even social harmony to develop a cancer treatment system that is non-toxic, efficient, safe and coexistent with multiple treatments. [0003] At present, the commonly used methods for the treatment of malignant tumors are chemotherapy and radio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/34A61K41/00A61P35/00
CPCA61K9/06A61K41/0057A61K47/34A61P35/00
Inventor 杨洪雨付雁刘长玲佀庆波宋岩
Owner JILIN INST OF CHEM TECH
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