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Lignin magnetic nanoparticles with pH response, preparation method thereof and application of lignin magnetic nanoparticles in cellulase recycling

A technology for the recovery of magnetic nanoparticles and cellulase, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of not exerting the value of lignin, environmental pollution, difficulties, etc., to promote development and high-value utilization , Widening the scope of application, the effect of high recovery rate

Inactive Publication Date: 2019-03-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, these studies all aim to adsorb and immobilize cellulase on magnetic nanomaterials, and then use the combination of enzyme and carrier to act on the substrate without decomposing cellulase. In this way, cellulase hydrolyzes wood fiber There are still great difficulties and obstacles in the process of
[0009] Lignin is a natural macromolecule second only to cellulose in nature. Every year, industrial lignin, a by-product of the paper industry in my country, is piled up as waste or directly incinerated to obtain a low calorific value. Its own value, there are still problems such as environmental pollution

Method used

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  • Lignin magnetic nanoparticles with pH response, preparation method thereof and application of lignin magnetic nanoparticles in cellulase recycling
  • Lignin magnetic nanoparticles with pH response, preparation method thereof and application of lignin magnetic nanoparticles in cellulase recycling
  • Lignin magnetic nanoparticles with pH response, preparation method thereof and application of lignin magnetic nanoparticles in cellulase recycling

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Experimental program
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Embodiment 1

[0060] Parts by mass, g / mL:

[0061] Prepare 350 parts by mass of stone inkstone wood sodium aqueous solution (wood sodium content 20wt.%) at pH=12, after heating to 85°C, adjust the speed of the peristaltic pump to 6mL / min, and use the peristaltic pump to mix 43.08 parts by mass (3-chloro-2- Hydroxypropyl) trimethylammonium chloride (CHPTAC) solution (content 65wt.%) slowly drips, adds 14.88 mass parts 20wt.% sodium hydroxide aqueous solution when dripping 5min, after continuing to drop CHPTAC solution again, in React at 85°C for 4 hours, dialyze the reaction solution in deionized water with a dialysis membrane with a molecular weight of 1000, remove unreacted CHPTAC and small molecular impurities, and obtain the product concentrate (SL-N40) after rotary evaporation.

[0062] Dissolve ferric chloride and ferrous sulfate heptahydrate with a stoichiometric ratio of 2:1 in water, stir vigorously in a nitrogen atmosphere at a stirring rate of 450rpm, and slowly add 30 parts by vo...

Embodiment 2

[0065] Prepare 350 parts by mass of stone inkstone wood sodium aqueous solution (wood sodium content 20wt.%) at pH=12, after heating up to 85°C, adjust the speed of the peristaltic pump to 1.5mL / min, and 10.77 parts by mass (3-chloro-2 - Hydroxypropyl)trimethylammonium chloride (CHPTAC) solution (content 65wt.%) was slowly added dropwise, and 3.72 parts by mass of 20wt.% sodium hydroxide aqueous solution was added during the dropwise addition for 5 minutes, and then after the CHPTAC solution was continued to be added dropwise, React at 85°C for 4 hours, dialyze the reaction solution in deionized water with a dialysis membrane with a molecular weight of 1000, remove unreacted CHPTAC and small molecular impurities, and obtain the product concentrate (SL-N10) after rotary evaporation.

[0066] Dissolve ferric chloride and ferrous sulfate heptahydrate with a stoichiometric ratio of 2:1 in water, stir vigorously in a nitrogen atmosphere at a stirring rate of 400rpm, and slowly add 3...

Embodiment 3

[0069] Prepare 350 parts by mass of stone inkstone wood sodium aqueous solution (wood sodium content 20wt.%) at pH=12, after heating up to 85°C, adjust the speed of the peristaltic pump to 12mL / min, and use the peristaltic pump to mix 86.15 parts by mass (3-chloro-2- Hydroxypropyl)trimethylammonium chloride (CHPTAC) solution (content 65wt.%) is slowly added dropwise to the flask, and 29.77 mass parts of 20wt.% sodium hydroxide aqueous solution are added during the dropwise addition of 5 minutes, and then the CHPTAC solution is continued to be added dropwise Finally, react at 85°C for 4 hours, dialyze the reaction solution in deionized water with a dialysis membrane with a molecular weight of 1000, remove unreacted CHPTAC and small molecular impurities, and obtain the product concentrate (SL-N80) after rotary evaporation .

[0070] Dissolve ferric chloride and ferrous sulfate heptahydrate with a stoichiometric ratio of 2:1 in water, stir vigorously in a nitrogen atmosphere at a...

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Abstract

The invention belongs to the technical field of cellulase recycling and discloses lignin magnetic nanoparticles with pH response, a preparation method thereof and application of the lignin magnetic nanoparticles in cellulase recycling. The preparation method includes the steps of (1) subjecting a lignosulfonate and a quaternization reagent to reaction to obtain a lignin derivative with pH response; (2) subjecting the lignin derivative with pH response, a ferric salt and a ferrous salt to chemical co-precipitating reaction in the presence of a precipitating agent to obtain lignin magnetic nanoparticles with pH response. The invention also provides lignin magnetic nanoparticles with pH response prepared via the preparation method, and application of the lignin magnetic nanoparticles with pHresponse in cellulase recycling. The lignin magnetic nanoparticles with pH response are efficient in adsorbing and fixing cellulase and desorbing the cellulase by simple pH adjustment so as to efficiently recycle the cellulase; the recycled cellulase features high recycle rate and high activity and is reusable.

Description

technical field [0001] The invention belongs to the technical field of cellulase recovery, and in particular relates to a pH-responsive lignin magnetic nanoparticle, a preparation method thereof and an application in cellulase recovery. Background technique [0002] The world today still uses fossil resources and fossil fuels as the main energy sources. The current petrochemical industry development model is unsustainable. Fossil resources as non-renewable resources cause resource crises, and fossil fuels as non-renewable energy sources cause energy crises. The discharge of three wastes leads to the destruction of the ecological environment and causes an environmental crisis. Therefore, the development and efficient utilization of biomass resources is an inevitable choice for human sustainable development. The use of cellulase to catalyze the hydrolysis of cellulose to convert it into fuel ethanol is an important way for the efficient utilization of biomass. However, in th...

Claims

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Application Information

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IPC IPC(8): C08H7/00C12N9/42
CPCC08H6/00C12N9/2437C12Y302/01004
Inventor 邱学青董芮璟郑大锋
Owner SOUTH CHINA UNIV OF TECH
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