Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of effective inhibitor of III-type phosphatidylinositol phosphate kinase in preparing drug for treating or preventing influenza virus infection

A type of influenza A virus, virus infection technology, applied in the field of medicine, can solve problems such as undiscovered

Active Publication Date: 2019-03-12
浙江安维珞诊断技术有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no information about 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidine Related reports of -2-yl]phenyl]-3-pyridinecarboxamide against influenza virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of effective inhibitor of III-type phosphatidylinositol phosphate kinase in preparing drug for treating or preventing influenza virus infection
  • Application of effective inhibitor of III-type phosphatidylinositol phosphate kinase in preparing drug for treating or preventing influenza virus infection
  • Application of effective inhibitor of III-type phosphatidylinositol phosphate kinase in preparing drug for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl Evaluation of the anti-influenza virus activity of ]phenyl]-3-pyridinecarboxamide

[0029] 1. Experimental materials

[0030] 1.1 Cells, viruses and drugs

[0031] MDCK cells were purchased from ATCC, and the virus A / PuertoRico / 8 / 34 (H1N1) was amplified from chicken embryo culture, 6-amino-N-[3-[4-(4-morpholino)pyrido[3', 2':4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide (CAS: 371942-69-7) was purchased from Sigma Company.

[0032] 1.2 Experimental Instruments

[0033] Multifunctional detector PerkinElmer, inverted microscope Costar.

[0034] 2. Experimental methods and results

[0035] 2.1 Cell culture

[0036] 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured to 90% confluence, and the subculture ratio was 1 / 3–1 / 4.

[0037] 2.2 Virus culture

[0038] Take ...

Embodiment 2

[0049] Example 2: 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl Evaluation of broad-spectrum anti-influenza virus of ]phenyl]-3-pyridinecarboxamide

[0050] On the basis of Example 1, this example further detects 6-amino-N-[3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3 ,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide to influenza A virus subtype H1N1 amantadine-resistant strains, influenza A virus subtype H6N6 strains, influenza A virus subtype Antiviral activity of H7N8 strain and influenza B virus.

[0051] 1. The virus strains used in the experiment include: A / WSN / S31N (H1N1), A / Duck / Hubei / 5 / 2010 (H6N6), A / Duck / Hubei / 216 / 1983 (H7N8), B / human / Huber / 1 / 2007

[0052] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. The culture medium in the well plate was discarded, washed twice with PBS, 5....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Half inhibitory concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses application of effective inhibitor of III-type phosphatidylinositol phosphate kinase in preparing drug for treating or preventing influenza virus infection. Research results prove that the effective inhibitor, namely a small molecule compound: 6-amino-N-[3-[4-(4-morpholinyl)pyridine[3', 2':4, 5] furan [3, 2-d] pyrimidine-2-base] phenyl]3-pyridine carboxamide can inhibit copying of influenza A virus subtype H1N1 amantadine drug-tolerant strain, influenza A virus subtype H6N6 strain, influenza A virus subtype H7N8 strain and influenza B virus in a dosage-dependent mannerwithin a nontoxic range, thereby having great wide-spectrum influenza virus resistant activity. Therefore, the small molecular compound is safe, efficient and little in toxic and side effect during clinical treatment of influenza.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an effective inhibitor of type III phosphatidylinositol phosphokinase 6-amino-N-[3-[4-(4-morpholino)pyrido[3',2' : Application of 4,5]furo[3,2-d]pyrimidin-2-yl]phenyl]-3-pyridinecarboxamide in the preparation of medicines for treating or preventing influenza virus infection. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full length of the viral genome is about 13.6kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/519A61P31/16
CPCA61K31/519A61P31/16
Inventor 邬开朗刘芳祝成亮张艳朱莹刘兴晖
Owner 浙江安维珞诊断技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com