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Application of dithranol to preparation of medicament for treating or preventing influenza virus infection

A technology for the prevention of influenza and virus infection, applied in the field of medicine, can solve the problem of anti-influenza virus of undiscovered anthranol, and achieve the effect of broad-spectrum anti-influenza virus activity

Inactive Publication Date: 2015-05-13
武汉威立得生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no relevant reports about the anti-influenza virus of dithranol have been found

Method used

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  • Application of dithranol to preparation of medicament for treating or preventing influenza virus infection
  • Application of dithranol to preparation of medicament for treating or preventing influenza virus infection
  • Application of dithranol to preparation of medicament for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Evaluation of dithranol anti-influenza virus activity

[0034] 1. Experimental Materials

[0035] 1.1 Cells, viruses and drugs

[0036] MDCK cells were purchased from American Type Culture Collection (ATCC); virus strain: A / PuertoRico / 8 / 34 (H1N1); drug: dithranol was purchased from Sigma.

[0037] 1.2 Experimental Instruments

[0038] Multilabel microplate reader (Perkin Elmer).

[0039] 2. Experimental methods and results

[0040] 2.1 Cell culture: 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3 – 1 / 4.

[0041] 2.2 Virus culture: Take SPF chicken embryos aged 9 to 11 days, check them with an egg tester before inoculating the virus, and mark them at a position far away from the embryos, disinfect and punch holes, and inoculate 2- 4 The virus liquid with hemagglutination ti...

Embodiment 2

[0051] Example 2: Evaluation of broad-spectrum anti-influenza virus effect of dithranol

[0052] In this experiment, dithranol was tested against influenza A subtype H1N1 strains, influenza A virus subtype H3N2 strains, influenza A virus subtype H1N1 amantadine-resistant strains, influenza A virus subtype H6N6 strains, and influenza A virus subtype H6N6 strains. Antiviral activity of virus subtype H7N8 strain, influenza A virus subtype H9N2 strain and influenza B virus.

[0053] 1. The virus strains used in the experiment include: A / Human / Hubei / 1 / 2009(H1N1), A / human / Hubei / 3 / 2005(H3N2), A / human / WSN / 33 / (H1N1,S31N), A / Duck / Hubei / 5 / 2010(H6N6), A / Duck / Hubei / 216 / 1983(H7N8), A / Chicken / Jiangsu / 1 / 2005(H9N2), B / human / Hubei / 1 / 2007.

[0054] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the medium in the well plate,...

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PUM

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Abstract

The invention discloses application of dithranol to preparation of a medicament for treating or preventing influenza virus infection. Firstly, the toxicity of the dithranol to MDCK (Madin Darby Canine Kidney) cells is detected, and the result indicates that the dithranol is completely nontoxic to the cells in a dissolution concentration range. Secondly, the antivirus activity of the dithranol is measured in a completely non-toxic concentration range, and the result indicates that the small molecular compound has remarkable antivirus activity, and the antivirus function is dosage-dependent. Lastly, the antivirus activity of the dithranol to different types and subtypes of influenza viruses is detected, and the result indicates that the dithranol can inhibit replication of all detected influenza virus strains in a dosage-dependent way, and the anti-influenza virus activity of the dithranol is broad in spectrum. Thus, the dithranol disclosed by the invention is a novel broad-spectrum anti-influenza virus medicament, and can be used for preparing the medicament for preventing or treating influenza virus infection.

Description

technical field [0001] The invention relates to the application of dithranol in the preparation of medicines for treating or preventing influenza virus infection, and belongs to the technical field of medicine. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full-length viral genome is about 13.6 kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and non-structural proteins (NS1). Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes according to the differ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A61P31/16
Inventor 陈绪林安利伟唐薇
Owner 武汉威立得生物医药有限公司
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