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Uses of essential oil of mother clove

A technology of volatile oil, mother cloves, applied in application, chemicals for biological control, fungicides, etc., to achieve the effects of volatile, simple operation and high efficiency

Active Publication Date: 2021-07-23
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no document disclosure related to the technical solution of the present invention

Method used

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  • Uses of essential oil of mother clove
  • Uses of essential oil of mother clove
  • Uses of essential oil of mother clove

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of female clove volatile oil

[0021] (1) Add 8 times the mass of water to 1 kg of crushed mother clove powder, soak for 2 hours, use steam to extract the volatile oil in mother cloves by means of heating and distillation, the extraction time is 6 hours, and condense to collect the effluent;

[0022] (2) Dry the collected volatile oil with anhydrous sodium sulfate to remove the contained water, put it into a brown sample bottle, seal it, and store it at -20°C until use.

Embodiment 2

[0023] Embodiment 2: the activation of pathogenic bacteria

[0024] (1) The pathogenic bacteria isolated from the roots and shoots of Panax notoginseng were purified and identified as Fusarium oxysporum ( Fusarium oxysporum ), Fusarium solani ( Fusarium solani ), Pythium ( Pythium aphanidermatum ), Cylindrosporium destroya ( Cylindrocarpon destructans ), Botrytis ( Botrytis cinerea ), Glosporum anthracnose ( Colletotrichum gloeosporioides ) and Rhizoctonia solani ( Rhizoctonia solani ) strains were inoculated on PDA medium for activation, and after inoculation 4 times, the vigorously growing strains were taken for use;

[0025] (2) Potato dextrose agar (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water.

Embodiment 3

[0026] Embodiment 3: GC-MS measures the chemical composition of mother clove volatile oil

[0027] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS30m × 250μm × 0.25μm;

[0028] (2) EI source Ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30~500m / z; inlet temperature: 285°C; split ratio 10:1; injection volume: 1 μl; Electron energy: 70eV; column oven temperature rise program: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the continuous carbon (C9-C25) of alkanes is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;

[0029]

[0030] In the table: a: retention time; b: relative peak area; ± relative deviation; c: retention index.

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Abstract

The invention discloses the use of volatile oil of clove; that is, its application in agriculture to prevent and treat root rot of Chinese medicinal materials or crops or to prevent and control pathogenic bacteria that cause diseases in the aboveground parts of Chinese medicinal materials or crops; experimental results It shows that the volatile oil of mother clove can inhibit the mycelial growth of the four main pathogenic bacteria Fusarium oxysporum, Fusarium solani, Pythium and Cylindrospora destroyer that cause root rot of Panax notoginseng, and can completely inhibit the growth of fungi that cause root rot of Panax notoginseng. The three kinds of pathogenic bacteria that occurred in some diseases inhibited the growth of mycelia of Botrytis cinerea, G. anthracnose and Rhizoctonia solani; The compounding of methazol has a synergistic effect on multiple strains; due to the volatile nature of volatile oil and its aromatic and anti-pollution characteristics, the chemical fertilizers or pesticides developed with it will have the advantages of safety, effectiveness, and low residue.

Description

technical field [0001] The invention relates to the application of the volatile oil of mother clove, in particular to the control effect of the volatile oil of mother clove on root rot of Chinese medicinal materials or crops and various diseases of the aboveground parts of Chinese medicinal materials or crops, and belongs to the technical field of biopesticides. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, is a perennial herb of Araliaceae ginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain. It is a rare and precious cultivated medicinal plant unique to my country. , which has a cultivation history of more than 400 years in Wenshan Prefecture, Yunnan Province. The roots, stems, leaves, and flowers of Panax notoginseng can be used as medicine. The range of medicines is very wide, and the social demand is constantly increasing. With the rapid increase of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/28A01P3/00
CPCA01N65/00A01N65/28
Inventor 董鲜程永现徐福荣马玉楠
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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