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Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof

A technology of protein gel and mutants, which is applied in the medical field to achieve the effects of promoting hyperplasia, shortening the recovery period, and relieving pain

Pending Publication Date: 2019-03-08
MUDANJIANG YOUBO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention aims to propose a protein gel containing MG53 protein / MG53 mutant protein, which is used to solve the stability of MG53 protein, and ensure that the protein activity of the MG53 protein / MG53 mutant protein protein gel is stored at room temperature for a long time. Guarantee, effectively ensure the healing of diabetic foot, decubitus, burns and other wound surfaces

Method used

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  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof
  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof
  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] An MG53 protein / MG53 mutant protein-containing gel comprises the following components: gel matrix, preservatives, antioxidants, and MG53 protein / MG53 mutant protein freeze-dried powder.

[0054] The gel base includes: deionized water, sodium carboxymethyl cellulose, hydroxyethyl cellulose, glycerol acrylate / acrylic acid copolymer, SCLEROTIUM ROLFSSII gum.

[0055] The preservatives include: one or more of ethyl paraben, sodium benzoate, potassium sorbate, methylparaben, and propylparaben. The present invention is preferably: methylparaben and propylparaben. one or both.

[0056] Antioxidants include: EDTA-2Na (disodium ethylenediaminetetraacetate).

[0057] The moisturizing agent includes: one or more of sodium hyaluronate, glycerin, 1,2-pentanediol and butanediol.

[0058] The protein protective agent includes: one or more of glycerol, propylene glycol, human albumin, dextran and trehalose.

[0059] MG53 protein / MG53 mutant protein lyophilized powder includes: MG53 ...

Embodiment 2

[0061] The difference between this embodiment and the above-mentioned embodiment is that a gel containing MG53 protein / MG53 mutant protein has the following formula and ingredients: 55w / w%-66w / w% gel matrix, 3w / w%-5w / w% preservative, 5w / w%-10w / w% antioxidant, 2w / w%-3w / w% humectant, 5w / w%-7w / w% protein protectant, 20w / w%-25w / w% MG53 protein / MG53 mutant protein lyophilized powder.

[0062] The components of the gel matrix include: 45w / w%-50w / w% deionized water, sodium carboxymethylcellulose, 0.2w / w% sodium carboxymethylcellulose, 5w / w%-10w / w % Glycerin, Acrylate / Acrylic Copolymer, Hydrolyzed Sclerotinia Gum

[0063] The components of the preservative include: one or both of 1.5w / w%-3w / w% methylparaben and 1.5w / w%-3w / w% propylparaben.

[0064] The components of the antioxidant include: 5w / w%-10w / w% EDTA-2Na.

[0065] The components of the moisturizing agent include: 0.5w / w%-3w / w% sodium hyaluronate, 0.5w / w%-3w / w% glycerin, 0.5w / w%-3w / w% 1,2-pentanedi One or more of alcohol,...

Embodiment 3

[0070] A preparation method of MG53 protein / MG53 mutant protein gel: comprising the following steps:

[0071] S1. Preparation of gel matrix:

[0072] Add deionized water to the main emulsifying pot, sprinkle hydroxyethyl cellulose powder during stirring, and stir for 10 minutes, then heat to 75°C while stirring until the solution is completely transparent; start to cool down to 45°C, and stir well The components in disodium EDTA, glycerol (and) glycerol acrylate / propylene copolymer (and) propylene glycol sodium hyaluronate are sequentially added to the main emulsification pot to ensure that one component is completely dissolved and then the next component is added; finally a gel is obtained matrix.

[0073] S2. Mix the gel matrix, preservatives, antioxidants, humectants, and protein protectants according to a certain proportion to form a first mixed solution, and sterilize the first mixed solution by autoclaving, and the conditions of autoclaving are: 121° C. ,30 minutes.

...

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Abstract

The invention provides a gel containing MG53 protein / MG53 mutant protein as well as a preparation method thereof and application thereof, and belongs to the technical field of medicines. The gel containing the MG53 protein / MG53 mutant protein is prepared from the following components: a gel substrate, a preservative, an anti-oxidant, a protein protector, and MG53 protein / MG53 mutant protein freeze-dried powder. According to the gel containing the MG53 protein / MG53 mutant protein and the preparation method thereof provided by the invention, the protein active ingredients of the MG53 protein / MG53 mutant protein can be kept stable under a long-term room-temperature storage condition; healing of wound surfaces such asdiabetic feet, bedsores, burns and scalds is effectively ensured; a restoration stage of a patient is shortened; and pains of the patient are effectively relieved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a gel containing MG53 protein / MG53 mutant protein and a preparation method and application thereof. Background technique [0002] The relative molecular weight of MG53 protein is 53000, and it is one of the family members of cardiac / skeletal muscle-specific three-domain tripartite motif (TRIM) consisting of 477 amino acids. After years of research, Weisleder et al. (Weisleder et al., 2012) found that the molecular structure of MG53 protein has 3 domains, from the N-terminus to the C-terminus are the zinc finger domain, 2 B-boxes, and 1 coiled-coil structure. , Zn is also one of the components, and its carboxyl terminal contains a SPRY structure (He et al., 2012). It is specifically expressed in skeletal muscle and cardiomyocytes. [0003] Diabetic foot and bedsore are common clinical skin ulcer diseases, which bring great pain to patients. The foot is a complex target organ i...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K9/06A61P3/10A61P17/02
CPCA61K38/1709A61K47/42A61P3/10A61P17/02A61K9/06
Inventor 梁亚龙
Owner MUDANJIANG YOUBO PHARMA CO LTD
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