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Method for establishing insulin resistance animal model

A technology of insulin resistance and animal models, applied in animal feed, additional food elements, applications, etc., can solve the problems of low stability of animal models, excessive difference in results, and differences in the degree of pathogenicity of modeling, so as to eliminate adverse reactions , low cost, and avoid the effect of model instability

Inactive Publication Date: 2019-03-08
浦江县欧立生物技术有限公司
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Problems solved by technology

However, this high-selenium-induced insulin resistance animal model is induced by a single high-selenium factor, and the stability of the animal model is not strong, and the single high-selenium factor used can easily lead to excessive differences in results, resulting in the existence of pathogenicity in the model. certain difference

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  • Method for establishing insulin resistance animal model
  • Method for establishing insulin resistance animal model

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Embodiment 1

[0021] The establishment method of insulin resistance animal model, including selection of mouse species, grouping and modeling, blood collection and detection, by simultaneously feeding high-sugar, high-fat and high-selenium feed and drinking fructose solution to cause glucose and lipid metabolism disorder, and then induce male mice to produce insulin Resistance, to obtain insulin resistance animal models. The high-sugar, high-fat and high-selenium feed of the present invention is prepared by adding lard, sucrose, cholesterol, sodium selenate, potassium sorbate and pig bile salt to conventional experimental animal feed; the fructose solution is prepared by adding distilled water of analytical grade. The specific construction process and test results are as follows:

[0022] 1. Experimental reagents and instruments

[0023] Mouse insulin enzyme-linked immunoassay kit, Beijing Keyingmei Technology Co., Ltd., batch number: 17072401. Cholesterol (TC), triglyceride (TG), low-den...

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Abstract

The invention discloses a method for establishing an insulin resistance animal model. According to the method, feed with high content of selenium, sugar and fat and low-concentration fructose drinkingwater are used for inducing inbred line C57BL / 6 mice with highly similar genetic backgrounds in order to cause glycolipid metabolism disorder of the mice and then induce the occurrence of insulin resistance, and then the insulin resistance animal model is established. Through unique proportioning of the feed, a pathogenesis process of the insulin resistance caused by the change of a dietary structure is highly simulated, comparison between the induced animal model and a control group shows that animals in a model group are obviously different from animals in the normal control group in intakeglucose tolerance, insulin tolerance, animal insulin resistance index and insulin sensitivity index, and it is indicated that the animals in the model group have obvious insulin resistance. The method has the advantages that the special feed is adopted for feeding in two modes, adverse reactions caused by chemical reagents are eliminated, a molding method is simple, the molding rate is high, thecost is low, and the reproducibility is high.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for establishing an insulin resistance animal model. Background technique [0002] The construction of animal models is a very important technique in medical research. Constructing a disease model similar to human disease can overcome the constraints of long incubation period, many influencing factors, slow development and complex pathogenesis of human disease, better reflect the law of disease occurrence and development, and facilitate the study of different diseases. Diseases that are easy to study in humans themselves. Commonly used animal models mainly include spontaneous animal models, transgenic animal models and experimental animal models. Spontaneous animal models and transgenic animal models have high application value, but the high cost of modeling and high requirements for technical breeding conditions limit the general application of these...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K50/50A23K20/20A23K20/168A23K20/163A23K20/158A23K20/105
CPCA01K67/02A23K20/105A23K20/158A23K20/163A23K20/168A23K20/30A23K50/50
Inventor 李冰
Owner 浦江县欧立生物技术有限公司
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