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Multifunctional nanoparticle for tumor fluorescence imaging and photothermal/photodynamic therapy

A photodynamic therapy and nanoparticle technology, applied in the field of biomedical materials, can solve the problems of cell damage, low laser energy, no cell killing effect, etc., and achieve the effect of increasing drug loading and improving curative effect.

Inactive Publication Date: 2019-03-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For phototherapy, the photosensitizer / photothermal therapy reagent is the core element of phototherapy, and only in the place where the photosensitizer / photothermal therapy reagent exists can the phototherapeutic effect be produced and damage the cells; since the laser energy used in the treatment is generally Low, in the absence of photosensitizers, pure laser irradiation has no killing effect on cells

Method used

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  • Multifunctional nanoparticle for tumor fluorescence imaging and photothermal/photodynamic therapy
  • Multifunctional nanoparticle for tumor fluorescence imaging and photothermal/photodynamic therapy
  • Multifunctional nanoparticle for tumor fluorescence imaging and photothermal/photodynamic therapy

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Mix distearoylphosphatidylcholine (DSPC), distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000), porphyrin lipid (PGL) and near-infrared dye DiR according to a certain molar ratio ( 20%: 10%: 50%: 20%), then adopt ethanol injection method, under 50 ℃ of water bath ultrasonic conditions, inject above-mentioned mixture into 0.8ml water; In the dialysis bag, dialyze for 2-4 hours, and obtain multifunctional nanoparticles (PGL-DiR NPs) for tumor fluorescence imaging and photothermal / photodynamic therapy after separation and purification. The morphology and particle size distribution of nanoparticles are shown in the attached figure 2 , 3 As shown, the nanoparticles are well dispersed in the aqueous solution, and the particle diameter is about 100nm.

Embodiment 2

[0027] Mix distearoylphosphatidylcholine (DSPC), distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000), porphyrin lipid (PGL) and near-infrared dye DiR according to a certain molar ratio ( 10%: 10%: 50%: 30%), then adopt ethanol injection method, under 50 ℃ of water bath ultrasonic conditions, inject above-mentioned mixture into 0.8ml water; In the dialysis bag, dialyze for 2-4 hours, and obtain multifunctional nanoparticles (PGL-DiR NPs) for tumor fluorescence imaging and photothermal / photodynamic therapy after separation and purification.

Embodiment 3

[0029] In order to evaluate the photothermal conversion ability of the multifunctional nanoparticles obtained in Example 1-2, the nanoparticles with different concentrations were irradiated with 760nm laser (1W / cm 2 , 5min), record the change of its temperature with time. attached Figure 4 It shows that the temperature of PBS and PGL NPs basically does not increase significantly after 5 minutes of laser irradiation, and the temperature of 20 μmol of PGL-DiR NPs (DiR equivalent) can increase by about 12 °C after 5 minutes of laser irradiation, indicating that DiR has a high photo heat conversion efficiency.

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Abstract

The invention relates to a multifunctional nanoparticle for tumor fluorescence imaging and photothermal / photodynamic therapy, and relates to a preparation method of the multifunctional nanoparticle and applications of the multifunctional nanoparticle in tumor diagnosis and treatment. structure diagram of the multifunctional nanoparticle for the tumor fluorescence imaging and photothermal / photodynamic therapy is represented by a figure which is shown in the description. Membrane components of the multifunctional nanoparticle include near infrared dyes used for fluorescence imaging and photothermal therapy, lipids containing photosensitizer functional groups and used for photodynamic therapy and conventional phospholipids. A ratio of photothermal and photosensitive medicine can be regulatedaccording to needs, and the medicine loading amount is greatly increased. Under the guidance of near-infrared fluorescence, photothermal and photodynamic therapy are combined to inhibit the growth oftumors so as to improve the treatment efficiency of the tumors.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a multifunctional nanoparticle for tumor fluorescence imaging and photothermal / photodynamic therapy, and its use in tumor diagnosis and treatment. Background technique [0002] Fluorescence imaging is an extremely important tool in molecular biology and medical research. Among them, the light absorption of biomolecules in the near-infrared region (wavelength 600-900nm) is the lowest, and the autofluorescence is the weakest. A large amount of infrared light can pass through tissues and skin and be detected. Therefore, its wavelength range is considered the "diagnostic window" of optical imaging. Its unique advantages are: ① high sensitivity; ② targeted imaging of various tumors can be realized through the design of different fluorescent probes; ③ it can provide real-time and dynamic imaging of tumors in vivo. [0003] Tumor phototherapy (phototherapy) has graduall...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K41/00A61P35/00
CPCA61K41/0052A61K41/0071A61K41/0076A61K49/0032A61K49/0084
Inventor 戴志飞梁晓龙徐云雪潘文斌
Owner PEKING UNIV
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