Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method and detection method of uplc characteristic map of Angelica dahurica

A technology of characteristic map and construction method, which is applied in the field of UPLC characteristic map construction of Angelica dahurica, can solve the problems of less common peaks in characteristic map and less research on flavonoid components, and achieve the effect of simple method, easy operation and improved accuracy

Active Publication Date: 2021-11-16
GUANGDONG YIFANG PHARMA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the traditional method of research on the characteristic spectrum of Angelica dahurica, it mainly involves methods such as HPLC, GC and RRLC. The research objects mainly involve the chemical components of volatile oils and coumarins. There are few studies on flavonoids, and the established characteristic spectrum has fewer common peaks. few

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method and detection method of uplc characteristic map of Angelica dahurica
  • Construction method and detection method of uplc characteristic map of Angelica dahurica
  • Construction method and detection method of uplc characteristic map of Angelica dahurica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This embodiment is a method for constructing a UPLC characteristic map of Angelica dahurica.

[0059] 1. Instruments and materials

[0060] Waters H-class UPLC, Waters PDA detector, Empower workstation; AgilentSB C 18 Chromatographic column (100mm×2.1mm, 1.8μm); ACQUITY UPLC I-Class ultra-high performance liquid phase time-of-flight high-resolution mass spectrometry system, data processing system is MarkerLynx 4.1 workstation; Mettler XP26 one-millionth balance (Mettler Toledo, Switzerland) Company); Milli-Q ultrapure water purification system (Millipore, USA); KQ5500DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.). Methanol and acetonitrile were of chromatographic grade (Merk); water was Mili-Q purified water; other reagents were of analytical grade.

[0061] Imperatorin (batch number: 1108206-201616) and isoimperatorin (batch number: 110827-201611) were provided by China National Institute for Food and Drug Control; Angelica sinensis (batch number: wk...

Embodiment 2

[0140] The present embodiment is a kind of detection method of Angelica dahurica medicinal material, and the steps are as follows:

[0141] 1. Chromatographic conditions

[0142] with Agilent SB C 18 (100mm×2.1mm, 1.8μm) is a chromatographic column, the flow rate is 0.35mL / min; the column temperature is 35°C; Gradient elution was performed as specified in Table 2 below.

[0143] Table 2 Gradient elution table

[0144]

[0145]2. Preparation of the sample solution to be tested: take the medicinal material of Angelica dahurica to be tested (S18), grind it finely, pass it through a No. 3 sieve, take about 0.5 g, accurately weigh it, place it in a stoppered conical flask, and accurately add dilute ethanol (volume concentration). 45-55% ethanol aqueous solution) 20mL, weighed, ultrasonically treated (300W, 50kHz) for 30min, allowed to cool, weighed again, used dilute ethanol to make up the lost weight, shake well, filter, and take the subsequent filtrate , that is.

[0146]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volume ratioaaaaaaaaaa
volume ratioaaaaaaaaaa
Login to View More

Abstract

The invention relates to a construction method and detection method of UPLC characteristic map of Angelica dahurica medicinal materials. The method comprises the following steps: preparation of a reference substance solution: weighing the imperatorin reference substance, adding a solvent to dissolve to obtain a reference substance solution; preparation of a test solution: weighing the medicinal material of Angelica dahurica, adding an extraction solvent for ultrasonication or reflux Extract, filter, and get the continued filtrate to obtain the described need testing solution; the establishment of the characteristic spectrum: absorb the described reference substance solution and the need testing solution respectively, inject into an ultra-high performance liquid chromatograph, and measure to obtain the described test solution. Characteristic spectrum; wherein the elution conditions adopted by the ultra-high performance liquid chromatography are: acetonitrile is used as mobile phase A, and 0.05-0.15% acetic acid aqueous solution is used as mobile phase B with a volume concentration; gradient elution is adopted. The characteristic map established by this method can reflect the characteristics of Angelica dahurica more comprehensively, and the method is accurate, reliable, reproducible and easy to operate.

Description

technical field [0001] The invention relates to research on the quality of traditional Chinese medicinal materials, in particular to a method for constructing and detecting a UPLC characteristic map of Angelica dahurica medicinal materials. Background technique [0002] Angelica is the Umbelliferae Angelica dahurica (Fisch.exHoffm.) Benth.et Hook.f. or Hang Angelica Angelica dahurica (Fisch.ex Hoffm.) Benth.et Hook.f.var.formosana (Boiss.) Shan et Yuan. dried root. It was first recorded in "Shen Nong's Materia Medica". It is acrid and warm. It returns to the stomach, large intestine and lung meridians. It is used for the treatment of cold, headache, brow and bone pain, nasal congestion and runny nose, rhinorrhea, biyuan, toothache, leucorrhea, sore swelling and pain, etc. [0003] It has been reported in the literature that the main chemical and medicinal components of Angelica are flavonoids and their derivatives, and volatile oils. Modern pharmacological studies have sh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 黎晓丽陈菊英雷桥仕索彩仙梁慧马瑞瑞吴文平杨晓东陈向东
Owner GUANGDONG YIFANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com