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Rose apple seed extractant having blood sugar or fat lowering effect and preparation method and application thereof

A technology for reducing blood lipids of Pu peach seeds, applied in the field of natural medicinal chemistry, can solve the problems that no in-depth research has been done, the research of Pu peach seeds is not deep enough, and the like, and achieve a remarkable effect.

Active Publication Date: 2019-03-01
JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, although there is a report that the extract of rose apple seeds can lower blood sugar, the research on the active ingredients in rose apple seeds is not deep enough, and only a simple study is done after the rose apple seeds are extracted by water or alcohol
And those skilled in the art know, contain a large amount of chemical components in rose apple seed, so which components or which effective parts have hypoglycemic effect, prior art does not do in-depth research

Method used

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  • Rose apple seed extractant having blood sugar or fat lowering effect and preparation method and application thereof
  • Rose apple seed extractant having blood sugar or fat lowering effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 rose apple seed extract

[0026] (1) Take rose apple seeds, heat and reflux extraction with ethanol for 2 times, each extraction for 1.5h, combine and concentrate the extract, remove the solvent to obtain the rose apple seed ethanol extract;

[0027] (2) Suspend the alcohol extract of rose apple seeds with water, then extract twice with an organic solvent, combine the extracts, concentrate and remove the solvent to obtain rose apple seed extracts, which have the effect of lowering blood sugar or blood fat;

[0028] The consumption ratio of rose apple seed described in step (1) and ethanol is 1g: 8mL;

[0029] The organic solvent described in step (2) is the mixed organic solvent that ethyl acetate and n-butanol form by volume ratio 2:1; The consumption ratio of described rose fruit seed ethanol extract and water is 1g: 20mL; Each extraction The volume ratio of the mixed organic solvent to water is 1:1 in the process.

Embodiment 2

[0030] The preparation of embodiment 2 rose apple seed extract

[0031] (1) Take rose apple seeds, heat and reflux extraction with ethanol for 2 times, each extraction for 1.5h, combine and concentrate the extract, remove the solvent to obtain the rose apple seed ethanol extract;

[0032] (2) suspending the alcohol extract of rose apple seeds with water, then extracting twice with an organic solvent, combining the extracts, concentrating and removing the solvent to obtain rose apple seed extracts;

[0033] (3) Put the prepared rose apple seed extract on a silica gel column, first use 3 times the volume ratio of the column volume as a mixed solvent of cyclohexane and ethyl acetate consisting of 93:7 to elute and remove impurities; then use 5 The mixed solvent composed of cyclohexane and ethyl acetate with a volume ratio of 87:13 times the volume of the column is eluted, and the eluted part is collected, concentrated and dried to obtain the described rose apple seed with hypogly...

Embodiment 3

[0065] Example 3 Evaluation of the hypoglycemic and hypolipidemic effect of rose apple seed extract

[0066] Male Kunming mice (KM), SPE grade, body weight (20±2) g, were purchased from Guangdong Experimental Animal Center. After adaptive feeding for 1 week, 10 mice were randomly selected as a blank group and fed with conventional feed, and the remaining mice were fed with high-sugar and high-fat feed (containing 20% ​​lard, 20% egg yolk powder, 10% sucrose, 2 % cholesterol, 1% cholate and 47% conventional feed). After 4 weeks, fasted for 8 hours, the mice in the blank group were given 40 mg / kg of pH 4.4 citric acid buffer for intraperitoneal injection 4 times, and the mice in the high-sugar and high-fat diet group were given 40 mg / kg of STZ solution (prepared in pH 4.4 citric acid buffer) Intraperitoneal injection 4 times, once a day, continue to feed the mice in each group with the same feed. One week after STZ injection, blood was taken from the tail vein of the mice to m...

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Abstract

The invention relates to the technical field of natural pharmaceutical chemistry and particularly discloses a rose apple seed extractant having a blood sugar or fat lowering effect and a preparation method and application thereof. The preparation method comprises the following steps that rose apple seeds are taken, and a rose apple seed extract is obtained through ethyl alcohol extraction; water is added to the rose apple seed extract for mixing and suspension, then extraction is executed 1-3 times with an organic solvent, and a rose apple seed extractant is obtained, namely the rose apple seed extractant having the blood sugar or fat lowering effect; the organic solvent is a mixed organic solvent prepared from ethyl acetate and n-butyl alcohol. The rose apple seed extractant prepared by adopting the method is enriched with a large amount of blood sugar and fat lowering effective ingredients and has the remarkable blood sugar and fat lowering effect.

Description

technical field [0001] The invention relates to the technical field of natural medicinal chemistry, in particular to a rose apple seed extract with hypoglycemic or hypolipidemic effect, a preparation method and application thereof. Background technique [0002] Diabetes is a metabolic disease characterized by chronic hyperglycemia caused by a variety of etiologies. The metabolic disorder of sugar, fat and protein in diabetic patients can lead to a series of chronic complications such as hypertension, kidney disease and vascular disease. Diabetes has become a A worldwide public health problem that seriously threatens human health. The latest 8th edition of Diabetes Atlas released by the International Diabetes Federation (IDF) pointed out that in 2017, about 425 million adults around the world suffered from diabetes, and it is estimated that by 2045, the number of diabetic patients may reach 629 million. 1 diabetes patient dies, and the number of people who die from diabetes ...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P3/10A61P3/06A61K131/00
CPCA61K36/61A61K2236/333A61K2236/39A61K2236/55A61P3/06A61P3/10
Inventor 张声源陈一村聂华庄远杯余思萍李云霞林大都
Owner JIAYING UNIV
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