Construction method and detection method of HPLC feature map of golden cypress medicinal material
A technology of characteristic map and construction method, which is applied in the field of construction of HPLC characteristic map of Phellodendron phellodendron, can solve the problems that berberine and phellodendron can not fully reflect the quality, and cannot fully reflect the material characteristics of traditional Chinese medicine decoction, so as to improve quality control Level, good separation, good reproducibility
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Embodiment 1
[0069] Embodiment 1, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal materials
[0070] The present embodiment provides a method for building the HPLC characteristic spectrum of Cortex Phellodendri medicinal material, comprising the steps of:
[0071] (1) Preparation of reference solution
[0072] Take 0.1g of Cortex Phellodendri reference medicinal material, put it in a stoppered Erlenmeyer flask, add 50ml of 50% (v / v) methanol solution, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter, and take The filtrate was used as the reference substance solution of the contrast medicinal material.
[0073] Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance were taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.
[0074] (2) p...
Embodiment 2
[0087] Embodiment 2, the determination of the HPLC characteristic map construction condition of Cortex Phellodendri medicinal material
[0088] 1 feature map
[0089] Phellodendron Phellodendri is the dry bark of Phellodendron Phellodendron, a plant of the Rutaceae Phellodendron genus, which is the base plant of traditional Chinese medicine Phellodendron Phellodendri. It mainly contains a variety of alkaloids such as berberine, magnolanine, phellodendron, palmitate and other alkaloids, as well as lactones, sterols, mucus, etc. Berberine alkaloids are the main active ingredients in Cortex Phellodendri, which have antibacterial effect, and Cortex Cortex is the characteristic component in Cortex Phellodendri. After research, HPLC was used to establish the fingerprint of Cortex Phellodendri, which provided an effective method for the quality control of Cortex Phellodendri.
[0090] 1.1 Instruments, reagents and reagents
[0091] Instruments: Waters high performance liquid chr...
Embodiment 3
[0239] Embodiment 3, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal materials
[0240] The present embodiment provides a kind of detection method of Cortex Phellodendri medicinal material, and the steps are as follows:
[0241] (1) Preparation of reference solution
[0242] Take 0.1g of Cortex Phellodendri reference medicinal material, put it in a stoppered Erlenmeyer flask, add 50% methanol 50ml, ultrasonically treat (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter, take the filtrate as the reference medicinal material substance solution. Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance were taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.
[0243] (2) preparation of need testing solution
[0244] Get 0.1g of the test produ...
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