Intra-tank inoculation method of L-hydroxyproline bacterial suspension

A technology of hydroxyproline bacteria and bacterial suspension, applied in the direction of fermentation, etc., can solve the problems of increasing waste liquid discharge, long strain preparation cycle, increasing manpower and material resources, and cross-contamination, so as to reduce waste liquid discharge and reduce Pollution opportunities, the effect of reducing manpower

Inactive Publication Date: 2019-02-19
SHANDONG JINYANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional inoculation method of L-Hydroxyproline bacteria is to use the bacteria in the cryopreservation tube to pass the slant for 12 hours, and then use the slant to inoculate the culture solution of multiple shake flasks for 8~10 hours, check the od value, and then put it into the tank Shake flask culture liquid strains, during this period, the strain preparation cycle is longer, not only that, but also increases the discharge of waste liquid, increases the manpower and material resources and the chance of cross-contamination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Use the bacterial suspension to inoculate into the shake flask culture medium, increase the air pressure in the fermenter to 0.069MPa, wait until the bubbles stop overflowing, then reduce the air pressure in the fermenter to 0.025MPa, and the bacterial suspension in the shake flask starts to press into In the fermenter, the pressure was repeatedly increased and decreased 4 times; the formula of the shake flask culture medium was: tryptone: 8.7g / L, yeast powder: 3.6g / L, sodium chloride: 8.9g / L. The storage period is 200d.

Embodiment 2

[0018] Use the bacterial suspension to inoculate into the shake flask culture medium, increase the air pressure in the fermenter to 0.068MPa, wait until the bubbles stop overflowing, then reduce the air pressure in the fermenter to 0.025MPa, and the bacterial suspension in the shake flask starts to press into In the fermenter, the pressure was repeatedly increased and decreased 4 times; the formula of the shake flask culture medium was: tryptone: 9.2g / L, yeast powder: 3.4g / L, sodium chloride: 9.2g / L. The storage period is 200d.

Embodiment 3

[0020] Use the bacterial suspension to inoculate into the shake flask culture medium, increase the air pressure in the fermenter to 0.070MPa, wait until the bubbles stop overflowing, then reduce the air pressure in the fermenter to 0.025MPa, and the bacterial suspension in the shake flask begins to press into In the fermenter, pressurize and depressurize repeatedly 5 times; the formula of the shake flask culture medium is: tryptone: 9.5g / L, yeast powder: 3g / L, sodium chloride: 9.4g / L. The storage period is 195d.

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PUM

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Abstract

The invention belongs to the technical field of bacterial inoculation and relates to an intra-tank inoculation method of L-hydroxyproline bacterial suspension, characterized in that a bacterial suspension is inoculated to a shaking culture medium in intra-tank inoculation manner; the shaking culture medium is formulated with 8-10 g / L of tryptone, 2-5 g / L of yeast powder, and 8-10 g / L of sodium chloride. Bacterial inoculation is performed with the bacterial suspension herein, manpower and material resources are decreased, activity of a bacterium is guaranteed to maximum extent, cross contamination is prevented, emission of wastewater is decreased, and shop extended reproduction is satisfied.

Description

technical field [0001] The invention relates to a tank inoculation method of L-hydroxyproline bacteria suspension, which belongs to the technical field of bacteria inoculation. Background technique [0002] L-Hydroxyproline or hydroxyproline is mainly found in the collagen of animals, and its content can reach about 10%. Its function is to strengthen the elasticity and toughness of connective tissue. In the field of food additives, hydroxyproline is often used as a beverage additive because it has a unique sweetness in a bitter taste, can improve the flavor of juice drinks, and also has skin repairing functions. In the field of cosmetic additives, because hydroxyproline has anti-oxidation and anti-radiation effects, it can eliminate oxidants and adjust the potential effect of redox state of cells, so as to maintain skin and delay aging, so it is an important additive of cosmetics. In the field of medicine, since hydroxyproline has two chiral centers, it is easy to derivatiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/24
CPCC12P13/24
Inventor 魏哲赵丽蒲刚李松
Owner SHANDONG JINYANG PHARMA
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