Nucleic acid releasing agent and nucleic acid on-site releasing method

Abstract

The invention provides a nucleic acid releasing agent, and belongs to the technical field of nucleic acid extraction. The nucleic acid releasing agent is a liquid preparation. In the nucleic acid releasing agent, volume percentage content of a nonionic surfactant is 0.2-2%, mass volume percentage content of trehalose is 0.01-2%, mass volume concentration of protease K is 0.1-5 mg / ml, concentrationof potassium chloride is 50-200 mmol / L, the concentration of guanidinium isothiocyanate is 20-100 mmol / L, the concentration of bovine serum albumin is 0.5-10 mmol / L, the concentration of ethylene diamine tetraacetic acid is 1-10 mmol / L, and the concentration of Tris-HCl is 0.2-5 mmol / L. The nucleic acid releasing agent further comprises the balance of water. The nucleic acid releasing agent is capable of eliminating a recovering step of nucleic acid, and avoiding nucleic acid losing or polluting. The nucleic acid releasing agent can be applied for realizing on-site releasing and detecting ofthe nucleic acid.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction, and in particular relates to a nucleic acid release agent and a nucleic acid release method on site. Background technique [0002] At present, nucleic acid detection has been widely used in many fields such as clinical medicine, inspection and quarantine due to its high sensitivity, strong specificity, easy operation, and short time required, especially nucleic acid amplification technologies such as PCR and isothermal amplification. However, nucleic acid amplification technology is more sensitive to interfering substances in clinical or directly obtained specimens, which may cause false negative test results. Therefore, sample nucleic acid extraction and purification are required before nucleic acid amplification. However, the extraction and purification of nucleic acids in samples has always been the most time-consuming and cumbersome process in the entire experiment, which seri...

AI Technical Summary

Problems solved by technology

The boiling extraction method requires multiple steps such as changing tubes and centrifugation, the sample processing time is long, and there are still a small amount of substances that inhibit amplification, which will have an inhibitory effect on subsequent amplification; the extraction effect of the spin column method is better, but there are many steps. Moreover, there is the disadvantage that nucleic acid is easily lost or polluted in this process; the magnetic bead method has simple extraction steps, high recovery rate, and easy automatic extraction, but the product is very expensive and is not widely used at present

[0003] In summary, the current nucleic acid extraction methods have the problems of large sample demand, long time, and poor extraction effect

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