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Method for detecting L-cysteine based on calcium-metal organic framework material as fluorescent probe

A metal-organic framework, cysteine ​​technology, applied in the field of highly selective detection of L-cysteine ​​in the fluorescence "off-on" mode, can solve the problem of no related reports, achieve good water solubility and avoid detection time Long, highly sensitive effect

Inactive Publication Date: 2019-01-29
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the selective detection of L-cysteine ​​content in biological fluids using metal-organic framework materials as fluorescent probes using the fluorescent "off-on" mode.

Method used

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  • Method for detecting L-cysteine based on calcium-metal organic framework material as fluorescent probe
  • Method for detecting L-cysteine based on calcium-metal organic framework material as fluorescent probe
  • Method for detecting L-cysteine based on calcium-metal organic framework material as fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation, Synthesis and Structural Characterization of Metal Organic Framework Complex Materials

[0056] (1) Weigh 141.6 mg Ca(NO 3 ) 2 4H 2 O and 42.1 mg (1,1':4',1"-terphenyl-3,3",5,5"-tetracarboxylic acid) organic ligand reagent were uniformly dispersed in 2 mL deionized water and 6 mL DMF Stir in medium for 0.5-1 h, then add 3-7 μL (0.33 M) of HNO 3 solution. Then the mixed solution was transferred to a stainless steel autoclave with a polytetrafluoroethylene liner, and heated at a temperature of 120-140 °C for 96-100 h. Finally, after cooling to room temperature for 48-50 h, the obtained product was washed several times with water and diethyl ether to obtain a colorless block crystal.

[0057] (2) Prepared MOF crystals, based on H 4 L 1 . The yield is 37%. C 31 h 32 Ca 1.5 N 3 o 11 Theoretical value of elemental analysis (%): C 54.54, H 4.73, N 6.15; experimental value of elemental analysis (%): C 54.79, H 4.96, N 6.15; it shows that the element co...

Embodiment 2

[0067] Prepare a series of standard stock solutions for subsequent experimental testing

[0068] (1) 100 mg L -1Preparation of metal-organic framework material suspension with a high concentration: Weigh 0.0060 g metal-organic framework material and dissolve it in 60 mL deionized water, use an ultrasonic cleaner to ultrasonically disperse for 5 minutes to form a uniform suspension, and place it in a dark place for later use.

[0069] (2) Preparation of 0.1 M Tris-HCl buffer solution: Weigh 0.7880 g tris-hydrochloric acid, dissolve it in 40 mL deionized water and shake well to dissolve it completely, then add NaOH solution (0.5 M), use a pH meter to adjust the pH to 7.2, and finally make it to 50 mL with deionized water, and put it in the refrigerator for use.

[0070] (3) Preparation of 8 mM lead chloride solution: Accurately weigh 0.0178 g of lead chloride analysis reagent and dissolve it in 8 mL of deionized water, shake well to dissolve it completely, and prepare a series ...

Embodiment 3

[0073] Determination of Ultraviolet Absorption Spectrum and Fluorescence Emission Spectrum of Metal Organic Framework Materials Used in the Present Invention

[0074] Add 400 μL metal-organic framework material suspension (100 mg L -1 ), 400 μL of Tris-HCl buffer solution (pH=7.2, 0.1 M) was adjusted to 4 mL with deionized water, and the concentration of MOF in the final system solution was 10 mg L -1 . The absorbance of the system solution was detected by a UV-visible spectrophotometer; the absorbance of the system solution was measured by a fluorescence spectrophotometer at an excitation wavelength of 282 nm, an excitation slit of 5 nm, an emission slit of 5 nm, and a photomultiplier tube voltage of 560 V. Fluorescence emission spectrum. Such as image 3 As shown, 1 is the ultraviolet light absorption spectrum diagram, and 2 is the fluorescence emission spectrum diagram. From image 3 It can be seen that metal-organic framework materials have strong ultraviolet absorpti...

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Abstract

The invention discloses a method for detecting L-cysteine based on a calcium-metal organic framework material as a fluorescent probe. The invention firstly uses the lead chloride as a quenching agentto realize the fluorescence quenching of the Ca-MOFs complex, and then adding L-cysteine to achieve the fluorescence recovery effect on a quenching system, thereby indirectly determining the L-cysteine content in the system. The fluorescent probe Ca-MOFs complex used in the invention is a porous coordination polymer material with good water solubility, high chemical stability and low toxicity, isbased on Ca(NO3)2.4H2O as an inorganic ion node, uses rigidly symmetric (1,1':4',1'-terphenyl-3,3',5,5'-tetracarboxylic acid) as an organic bridging ligand, and uses a simple "one-pot" solvothermal technical means to be prepared and synthesized. The chemical formula of the fluorescent probe Ca-MOFs complex is {[Ca1.5(HL1)(DMF)2].DMF}. The method further discloses the use of the metal organic framework material as the fluorescent probe for the detection and analysis of L-cysteine and the preparation process of the Ca-MOFs complex crystal material.

Description

technical field [0001] The invention belongs to the field of preparation and synthesis of metal-organic framework materials and fluorescence sensing, and specifically relates to a high-selectivity detection of L-cysteine ​​based on low-toxicity metal-organic framework materials as fluorescent probes using fluorescence "off-on" mode method. Background technique [0002] L-cysteine ​​is an indispensable amino acid in the human body. As an important biologically active thiol-containing compound, it plays a vital role in the function of biological cells. L-cysteine ​​helps to maintain intracellular redox activity, protein synthesis, detoxification, metabolism, intracellular signal transduction and gene regulation and other physiological activities. It is also a potential neurotoxin or a biomarker for disease diagnosis, which is closely related to the physiological regulation of human body mechanisms. Moreover, the content of L-cysteine ​​in the living body will directly affect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李妍杨斌张慧敏王璐丁斌
Owner TIANJIN NORMAL UNIVERSITY
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