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Sandalwood endophytic fungus derived diphenolic acid compound, preparation method and application thereof in preparation of antibacterial drugs

A technology of diphenolic acid and endophytic fungi, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as allergic reactions, blood system damage, superinfection flora, etc., and achieve good antibacterial Effects of activity, antimicrobial potential for clinical application

Inactive Publication Date: 2019-01-15
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these eight types of antibacterial drugs have certain clinical antibacterial effects, many of them have produced a series of adverse reactions and drug resistance after long-term use, such as nervous system damage, liver damage, kidney damage, blood system damage, gastrointestinal reactions, dual Infection or flora imbalance, allergic reaction, etc.

Method used

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  • Sandalwood endophytic fungus derived diphenolic acid compound, preparation method and application thereof in preparation of antibacterial drugs
  • Sandalwood endophytic fungus derived diphenolic acid compound, preparation method and application thereof in preparation of antibacterial drugs
  • Sandalwood endophytic fungus derived diphenolic acid compound, preparation method and application thereof in preparation of antibacterial drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing diphenolic acid compounds derived from endophytic fungi of sandalwood. The diphenolic acid compounds are isolated from the fermentation broth of the endophytic fungus Fusarium sp. of sandalwood. The sandalwood endophytic fungus Fusarium sp. is isolated from the heartwood of the sandalwood plant Santalum album.

[0029] The sandalwood fungus Fusarium sp. was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on January 26, 2018. The preservation number is GDMCC No: 60318, and the classification is named Fusarium sp. The address of the preservation unit is 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City.

[0030] The concrete preparation method of described diphenolic acid compound is as follows:

[0031] S1. Seed liquid culture of the sandalwood endophytic fungus Fusarium sp.: insert the sandalwood endophytic fungus Fusarium sp. into the seed medium, and cultivate it at 30°C for 6 days at a shaking tab...

Embodiment 2

[0034] Embodiment 2 (compound structural characterization)

[0035] Structural analysis of new compounds I, II and III was carried out, and the following experimental data were obtained:

[0036] New compound I:C 18 h 17 NO 6 , HRESI-MS: 342.0989 [M-H] - (calculated 342.0983); new compound II:C 19 h 17 NO 7 , HRESI-MS: 370.0931 [M-H] - (calculated value 370.0932);

[0037] New compound III:C 16 h 12 o 5 , HRESI: 285.0751 [M+H] + (calculated value 285.0758).

[0038] See Table 1 for the NMR data of compounds I, II and III.

[0039] Table 1 NMR data of compounds I, II and III (400MHz / 100MHz, ppm)

[0040]

[0041] a signal overlap

[0042] According to above-mentioned data result, confirm that the structural formula of compound I, II and III is as follows:

[0043]

Embodiment 3

[0045] Antibacterial test of compounds I, II and III

[0046] Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus albus,

[0047] Micrococcus luteus, Bacillus cereus, and Salmonella were the test bacteria, and the antibacterial activity was determined by the filter paper method.

[0048] 1. Preparation of Bacterial Suspension

[0049] Activate the indicator strain on the LB plate, pick a ring of fresh slant cells in the sterilized LB broth, culture at 37°C with shaking at 120rpm / min overnight. Add sterile water to make a concentration of about 1×10 6 / mL of bacteria solution.

[0050] 2. Antibacterial test

[0051] The antibacterial activity was determined by the disc method. Add 200 μL of bacterial suspension on the plate and spread evenly

[0052] uniform. First use sterile tweezers to pick up a piece of sterile filter paper and stick it on the bacterial nutrient plate, and press gently to make full contact. The sample t...

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Abstract

The invention discloses a sandalwood endophytic fungus derived diphenolic acid compound, a preparation method and application thereof in preparation of antimicrobial drugs. The diphenolic acid compound has a structural formula shown as formula I, II or III. The new compound shows good bacteriostatic activity on two or more of multiple indicator strains. The MIC value is in the range of 3.0-12.5microg / mL, and compound can be used for preparation of antibacterial drugs. Therefore, the diphenolic acid compound provided by the invention has the potential of antibacterial clinical application.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical compounds, and more specifically relates to a class of diphenolic acid compounds derived from endophytic fungi of sandalwood, a preparation method thereof and an application in the preparation of antibacterial drugs. Background technique [0002] The resistance of Staphylococcus aureus to methicillin and the emergence of more and more pathogenic bacteria resistant to many existing antibiotics require us to continuously search for better antibacterial drugs. [0003] At present, there are eight main categories of antibacterial drugs commonly used clinically: lactams, macrolides, aminoglycosides, tetracyclines, lincomycins, polypeptides, sulfonamides, quinolones, etc. Although these eight types of antibacterial drugs have certain clinical antibacterial effects, many of them have produced a series of adverse reactions and drug resistance after long-term use, such as nervous system damage, live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C235/46C07D307/88A61P31/10C12P13/02C12P17/04C12R1/77
CPCA61P31/10C07C235/46C07D307/88C12P13/02C12P17/04
Inventor 闫冲刘红菊刘威杨李静
Owner GUANGDONG MEDICAL UNIV
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