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Method for extracting guaiane-type sesquiterpene compound from Cynara scolymus

A technology of guaiacane and artichoke, which is applied in the direction of organic chemistry, can solve the problems of limited separation and purification of active ingredients, and achieve the effect of high extraction purity, simple extraction method, efficient and controllable separation

Active Publication Date: 2019-01-11
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing technology has limited research on the separation and purification of its active ingredients.

Method used

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  • Method for extracting guaiane-type sesquiterpene compound from Cynara scolymus
  • Method for extracting guaiane-type sesquiterpene compound from Cynara scolymus
  • Method for extracting guaiane-type sesquiterpene compound from Cynara scolymus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into a 70% ethanol aqueous solution with a volume fraction of 1:10 for leaching for 72 hours. Stir constantly during the leaching, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract. The solid content of the crude extract is 0.1 g / mL.

[0046] Soak the AB-8 macroporous resin in absolute ethanol for 24 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until th...

Embodiment 2

[0052] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into a 70% ethanol aqueous solution with a volume fraction of 1:8 for extraction for 70 hours. Stir continuously during the extraction, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract.

[0053] Soak the AB-8 macroporous resin in absolute ethanol for 20 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until the resin in the lower layer of the column is all ...

Embodiment 3

[0058] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into an aqueous ethanol solution with a volume fraction of 70% for 71 hours at a material-to-liquid ratio of 1:9. Stir continuously during the extraction, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract.

[0059] Soak the AB-8 macroporous resin in absolute ethanol for 22 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until the resin in the lower layer of...

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Abstract

The present invention provides a method of extracting a guaiane-type sesquiterpene compound from Cynara scolymus. The method comprises the following steps: pulverizing Cynara scolymus and extracting with ethanol water to obtain crude extract liquor; loading the crude extract liquor to an AB-8 macroporous resin chromatographic column for elution, carrying out chromatographic detection, collecting an eluant containing a target compound, concentrating and freeze-drying to obtain a crude extract; dissolving the crude extract in 50% methanol, loading to an RP-C18 chromatographic column for elution,carrying out chromatographic detection, collecting an eluant containing the target compound, concentrating and freeze-drying to obtain a pre-separation product; dissolving the pre-separation productin an acetonitrile aqueous solution, carrying out liquid-phase semi-preparation, collecting a flow fraction containing the target compound, concentrating and freeze-drying to a quasi-extract; dissolving the quasi-extract with an aqueous methanol solution, carrying out liquid-phase semi-preparation, collecting the flow fraction containing the target compound, concentrating and freeze-drying to obtain the target compound. By the method provided by the application, the target compound is effectively separated from Cynara scolymus, and the extraction purity is high.

Description

technical field [0001] The invention relates to the field of extraction and purification, in particular to a method for extracting guaiac-type sesquiterpene compounds from artichokes. Background technique [0002] Artichoke is a perennial herb of the family Asteraceae, also known as artichoke, artichoke, chrysanthemum thistle, lotus lily, French lily, and its scientific name is Cynara scolymus L. Native to the Mediterranean coast, southern Europe and North Africa, it was introduced to Shanghai, China by France in the 19th century, and is now mainly distributed in Shanghai, Zhejiang, Yunnan, Hunan, Shandong and Beijing. Artichoke has a high edible value and has the reputation of "the king of vegetables". [0003] Artichoke leaf extract has been used in folk medicine for a long time. Its in vitro and clinical tests have shown that artichoke extract has antioxidant, antibacterial, anti-cancer and anti-tumor properties, liver protection, hypoglycemia, improvement of digestion a...

Claims

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Application Information

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IPC IPC(8): C07D307/93
CPCC07D307/93
Inventor 曹清明张晓帆岑嘉惠莫海玲刘子华
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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