Preparation and application of enzymatic hydrolysate from Grifola frondosa waste residue rich in soluble dietary fiber
A technology of dietary fiber and enzymatic hydrolysis products, which is applied in the field of preparation of enzymatic hydrolysis products of Grifola frondosa waste residue, can solve the problems of increased content and inefficient utilization of Grifola frondosa waste residue, so as to reduce fasting blood sugar, increase product added value, reduce Serum Total Cholesterol Effect
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Embodiment 1
[0029] The preparation method of the present embodiment comprises the following process steps:
[0030] A, raw material pretreatment: drying the waste slag of Grifola frondosa in an oven at 80°C, passing through a 60-mesh sieve after crushing;
[0031] B. Enzymolysis: Put the pretreated Grifola frondosa waste residue in an enzymolysis reactor, add water to boil according to the volume-to-weight ratio of 1:20, and cool to no more than 37°C. Add 0.5% of the mass of helicase to the cooled feed solution, adjust the pH value to 5.5, stir at 37°C for enzymolysis, and the enzymolysis time is 48h;
[0032] C. Enzyme inactivation: Stop stirring after the enzymolysis reaction is over, rapidly heat up the enzymolysis solution in step B to boiling, keep it for no less than 20 minutes, and inactivate the helicase;
[0033] D. Drying: Concentrate the enzymatic hydrolysis solution in step C under reduced pressure, and dry the enzymatic hydrolysis solution concentrated under reduced pressure...
Embodiment 2
[0036] The difference between this embodiment and embodiment 1 is:
[0037] B. Enzymolysis: Place the pretreated Grifola frondosa waste slag in the enzymolysis reactor, add water to boil with a volume-to-weight ratio of 1:20, and cool to 40°C. 1.5% Helicase was added to the cooled feed solution to adjust the pH value to 7, and the enzymolysis was stirred at 40° C. for 72 hours.
[0038] All the other contents are the same as in Example 1.
Embodiment 3
[0040] The difference between this embodiment and embodiment 1 is:
[0041] B. Enzymolysis: Place the pretreated Grifola frondosa waste slag in an enzymolysis reactor, add water to boil with a volume-to-weight ratio of 1:15, and cool to 35°C. 1.0% Helicase was added to the cooled feed solution to adjust the pH value to 6, and the enzymolysis was stirred at 35° C. for 48 hours.
[0042] All the other contents are the same as in Example 1.
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