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Application of cationic polyvinylamine linear macromolecules as transgenic vector

A technology of transgenic carrier and polyvinylamine, which is applied in the fields of polymer chemistry and biological materials, can solve the problems of low transfection efficiency, high price, and restrictions on the application of polycationic polymers, and achieve high transfection efficiency, low price, The effect of good biocompatibility

Active Publication Date: 2019-01-04
西安九清医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently commercially available polycationic polymers that can be used for nucleic acid delivery are expensive and have low transfection efficiency, which greatly limits the application of polycationic polymers.

Method used

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  • Application of cationic polyvinylamine linear macromolecules as transgenic vector
  • Application of cationic polyvinylamine linear macromolecules as transgenic vector
  • Application of cationic polyvinylamine linear macromolecules as transgenic vector

Examples

Experimental program
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Effect test

example 1

[0036] A cationic polyvinylamine linear polymer is used as a transgene vector, and the structural formula (I) of the linear polymer transgene vector is as follows:

[0037]

[0038] In formula (I), x / (x+y)=0.5-1. Its raw material is called XELOREX TM RS 1100.

[0039] For the linear polymer transgenic vector material (XELOREX TM RS 1100) has been purified as follows:

[0040] Using water as the dialysis medium, XELOREX TM RS 1100 aqueous solution (concentration is 10%) through MWCO8-14kd dialysis bag (through molecular weight <10000) Dialysis for 72h to remove small molecules that are toxic to cells in the raw materials. After dialysis, freeze-dry. The obtained powder is re-dissolved in three-distilled water and prepared into a stock solution of appropriate concentration for use.

[0041] The linear polymer transgene vector in Example 1 was tested as follows:

[0042] (1) Agarose gel electrophoresis test of the above linear polymer transgene vector and DNA complex

[0043] a. Prepa...

example 2

[0072] A cationic polyvinylamine linear polymer is used as a transgene vector, and the structural formula (I) of the linear polymer transgene vector is as follows:

[0073]

[0074] In formula (I), x / (x+y)=0.5-1. Its raw material is called XELOREX TM RS 1100.

[0075] For the linear polymer transgenic vector material (XELOREX TM RS 1100) has been purified as follows:

[0076] Using water as the dialysis medium, XELOREX TM RS 1100 aqueous solution (concentration is 10%) through MWCO8-14kd dialysis bag (through molecular weight <10000) Dialysis for 72h to remove small molecules that are toxic to cells in the raw materials. After dialysis, freeze-dry. The obtained powder is re-dissolved in three-distilled water and prepared into a stock solution of appropriate concentration for use.

[0077] The linear polymer transgenic vector in Example 2 was tested as follows:

[0078] (1) Transgenic vector cytotoxicity test

[0079] a. Preparation of transgenic vector and siRNA complex

[0080] At ...

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Abstract

The invention provides a cationic polyvinylamine linear macromolecular transgenic vector, commercially named XELOREXTM RS 1100. The invention provides application of the linear macromolecular transgenic vector as a nucleic acid molecular transport vector. The vector herein provides efficient transfection during cell transfection. The material cost is low; cell toxicity during transfection is low;genetic molecules can be transported to cells effectively and safely. The linear macromolecular transgenic vector has the advantages of high efficiency, low toxicity and low price.

Description

Technical field [0001] The invention belongs to the technical field of polymer chemistry and biological materials, and specifically relates to the application of a cationic polyvinylamine linear polymer. Background technique [0002] As a new type of treatment, gene therapy has shown great application potential in the treatment of major diseases such as cancer, AIDS, and osteoporosis. However, due to the lack of safe and efficient gene carriers, gene therapy has not made substantial progress in clinical practice. [0003] Gene therapy refers to the introduction of foreign normal genes into target cells with the help of gene vectors to correct or compensate diseases caused by gene defects or abnormalities, so as to achieve the purpose of treatment. However, naked DNA generally exists in the form of a stretched linear spiral, with loose volume, and the DNA molecule itself is a negatively charged anion. When it enters the cell, it will generate electrostatic repulsion with the anion ...

Claims

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Application Information

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IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 邓旭东田野尹大川骞爱荣高永光王雪李瑞云张托弟
Owner 西安九清医疗科技有限公司
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