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Sustained-release microspheres, protein glycosylation inhibitor sustained-release system and their preparation and application

A technology of protein glycosylation and slow-release microspheres, which is applied in the fields of application, non-active ingredients of polymer compounds, medical preparations of non-active ingredients, etc., can solve the problems of short time and poor inhibitory effect of protein glycosylation, etc. Achieve smooth and complete shape, improve sustained release effect, and good dispersion

Active Publication Date: 2020-12-25
JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of poor protein glycosylation inhibition effect and short time in the prior art, and provide a sustained-release microsphere with uniform dispersion, stable inhibition process, good effect and long inhibition time. Glycosylation inhibitor slow-release system and its preparation method and application

Method used

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  • Sustained-release microspheres, protein glycosylation inhibitor sustained-release system and their preparation and application
  • Sustained-release microspheres, protein glycosylation inhibitor sustained-release system and their preparation and application
  • Sustained-release microspheres, protein glycosylation inhibitor sustained-release system and their preparation and application

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Embodiment 1

[0059] This embodiment provides a protein glycosylation inhibitor sustained release system, including:

[0060] Core balls: epicatechin and fishbone microspheres; the particle size of the microspheres is 300nm, the porosity is 25%, and the pore size is 15nm;

[0061] The first sustained release layer: selenomethionine and carrageenan;

[0062] Second sustained-release layer: vitamin E, rose seed oil and whey protein isolate;

[0063] The preparation method of this embodiment is:

[0064] (1) Mix 1 g of epicatechin with 1 g of fishbone microspheres, dissolve in 100 ml of phosphate buffer solution with a pH value of 7 and a concentration of 10 mM and mix evenly, and use ultrasonic-guided vacuum technology to promote the embedding of epicatechin into For fishbone microspheres, the ultrasonic power is 500W, the vacuum degree is 0.1MPa, and the action time is 25min. It is filtered by a filter membrane with a molecular weight cut-off of 5000Da, and the retentate is used for later ...

Embodiment 2

[0069] This embodiment provides a protein glycosylation inhibitor sustained release system, including:

[0070] Core balls: selenomethionine and resistant starch microspheres; the particle size of the microspheres is 600nm, the porosity is 50%, and the pore size is 35nm;

[0071] First sustained release layer: catechin and carrageenan;

[0072] Second sustained-release layer: vitamin E, rose seed oil and whey protein isolate;

[0073] (1) The preparation method of this example is: take 1g of selenomethionine and 0.5g of resistant starch microspheres, dissolve them in 100ml of phosphate buffer solution with a pH value of 7 and a concentration of 10mM, mix them evenly, and use ultrasonic guided vacuum technology To promote the embedding of selenomethionine into fishbone microspheres, the ultrasonic power is 400W, the vacuum degree is 0.05MPa, the action time is 20min, and the filter membrane with a molecular weight cut-off of 5000Da is used for filtration, and the retentate is ...

Embodiment 3

[0078] This embodiment provides a protein glycosylation inhibitor sustained release system, including:

[0079] Core balls: microspheres made by mixing epicatechin, selenomethionine, soybean protein isolate and fish gelatin; the particle size of the microspheres is 280nm, the porosity is 22%, and the pore size is 14nm;

[0080] First slow-release layer: vitamin C and sodium alginate;

[0081] Second sustained-release layer: vitamin E, rose seed oil and whey protein isolate;

[0082](1) The preparation method of this embodiment is: take 0.5g epicatechin, 0.5g selenomethionine and 1.5g soy protein isolate and fish gelatin to make microspheres and mix them, dissolve them in pH value of 7, concentration is 10mM Mix well in 100ml of phosphate buffer solution, use ultrasonic-guided vacuum technology to promote embedding of epicatechin and selenomethionine into fish bone microspheres, ultrasonic power is 600W, vacuum degree is 0.15MPa, action time is 30min, molecular weight cut-off ...

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Abstract

The invention relates to the technical field of slow-release microspheres and in particular relates to a slow-release microsphere and protein glycosylation inhibitor slow-release system as well as preparation and application thereof. The slow-release microspheres comprise core spheres and at least two slow-release layers from inside to outside in sequence, wherein the surfaces of the core spheresare coated by the slow-release layers to form core-shell structures. According to characteristics of different stages of protein glycosylation, inhibitors for different stages are coated by differentslow-release layers by utilizing the slow-release microspheres, the slow-release microspheres are tight and reasonable in coating, large in coating capacity, regular and uniform in morphology, good indispersibility, stable in slow-release process and free of sudden release phenomenon, the inhibitors for early, middle and late stages are coated by the slow-release microspheres from outside to inside, the inhibition effect can be further improved, the slow-release time can be prolonged, modes of physical mixing, high-pressure electric fields, high pressure homogenization and the like are implemented as preparation methods, unnecessary chemical reagents are avoided, and the slow-release microspheres are simple and convenient in method, good in coating effect, good in practicability and applicable to fields such as foods, medicines and cosmetics.

Description

technical field [0001] The invention relates to the technical field of sustained-release microspheres, in particular to a sustained-release microsphere, a protein glycosylation inhibitor sustained-release system and its preparation and application. Background technique [0002] Protein glycosylation is the transfer of sugars to proteins under the action of glycosyltransferases, and the formation of glycosidic bonds with amino acid residues on proteins. Proteins undergo glycosylation to form glycoproteins and produce by-products. Glycosylation is an important modification of protein and can regulate the function of protein. However, in the process of food processing and storage, protein glycosylation not only affects the properties and nutritional value of food, but also produces harmful substances. It is a major factor that makes food go bad, so inhibiting protein glycosylation is of great significance for food preservation and quality assurance. [0003] In the process of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61K47/46A61K47/44A61K47/42A61K47/36A61K47/22A61K47/18A23L3/3544
CPCA23L3/3544A23V2002/00A61K9/1617A61K9/1652A61K9/1658A61K9/1664A61K9/167A23V2250/708A23V2250/712A23V2250/0632A23V2250/1626
Inventor 刘光宪张诚戴天放王辉付晓记熊慧薇幸胜平
Owner JIANGXI ACAD OF AGRI SCI
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