Application of Bletilla striata oligosaccharides in improving intestinal microecology
A technology of intestinal microecology and Bletilla striata oligosaccharide, which can be used in medical preparations containing active ingredients, non-central analgesics, extracellular fluid diseases, etc. And other issues
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Embodiment 1
[0037] Example 1 Preparation of Bletilla striata oligosaccharides by strong acid method
[0038] In this embodiment, a method for preparing bletilla striata oligosaccharides by strong acid hydrolysis is provided, and the specific implementation steps are as follows:
[0039] (1) Take 25g of Bletilla striata tubers and grind them into 80-mesh powder, add 500ml of water to mix, dissolve and extract at 80°C for 8 hours to obtain an extract;
[0040](2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 50ml;
[0041] (3) Add 150 ml of 95% ethanol to the concentrated solution in step (2), and let stand overnight for 12 hours.
[0042] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;
[0043] (5) After the precipitate described in step (4) is redissolved, add trifluoroacetic acid (final concent...
Embodiment 2
[0044] Example 2 Preparation of Bletilla striata oligosaccharides by enzymatic hydrolysis
[0045] In this embodiment, a method for preparing bletilla striata oligosaccharides by polysaccharide hydrolase is provided, and the specific implementation steps are as follows:
[0046] (1) Take 40g of Bletilla striata striata powder, add 600ml of water to mix, dissolve and extract at 40°C for 12 hours to obtain an extract;
[0047] (2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 60ml;
[0048] (3) Add 200 ml of 90% ethanol to the concentrated solution in step (2), and let stand overnight.
[0049] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;
[0050] (5) Redissolve the precipitate in step (4) with 50ml of water, add 1g of mannanase, hydrolyze at 40°C for 4 hours at pH 6.8, add a certai...
Embodiment example 1
[0053] Implementation Case 1 Bletilla striata oligosaccharides reduce DSS-induced colitis
[0054] (1) In this embodiment, in order to evaluate the improvement effect of bletilla striata oligosaccharides on colitis mice, the specific implementation steps are as follows:
[0055] (2) 4-week-old C57BL / 6J mice were taken, fed with normal standard feed and sterile drinking water, and the mice were adapted to the new environment for 1 week.
[0056] (3) To start the experiment, the mice were treated with 3.5% dextran sodium sulfate (DSS) by intragastric administration, and at the same time, 2% Bletilla striata oligosaccharides were administered by intragastric administration. The processing time is 10 days.
[0057] (4) Detect drinking water, diet, body weight, and stool fineness during treatment.
[0058] (5) Summarize the disease scores of the mice and perform histological scoring.
[0059] The experimental results are attached image 3 .
[0060] The results showed that aft...
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