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A synergistic composition used for rice sheath blight control and application thereof

A technology for rice sheath blight and rice sheath blight, which is applied in the field of prevention and control of rice sheath blight, can solve problems such as drug resistance, and achieve the effects of expanding the bacteriostatic spectrum, delaying the drug resistance of pathogens, and reducing drug residues

Inactive Publication Date: 2018-12-18
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Jinggangmycin has been an important agent for controlling rice sheath blight, but there is a risk of drug resistance in long-term large-scale application

Method used

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  • A synergistic composition used for rice sheath blight control and application thereof
  • A synergistic composition used for rice sheath blight control and application thereof
  • A synergistic composition used for rice sheath blight control and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Indoor Toxicity Assay

[0018] (1) Drug quality setting

[0019] In the aseptic operation bench, dissolve the test agents phenazine-1-carboxamide and fluazinam with a small amount of acetone organic solvent respectively, and prepare a mother solution with a mass concentration of 100 μg / mL with sterile water. Different agents have different growth rates of mycelium. Each agent needs to be pre-tested, and three mass concentrations of 100, 10, and 1 μg / mL are set, and sterile water is used as a control, so as to obtain a mass with an inhibition rate of 10% to 85%. Concentration range, set 5 mass concentration gradients for experiments, for rice sheath blight bacteria, the corresponding mass concentrations of phenazine-1-carboxamide and fluazinam are: 10, 1, 0.1, 0.01, 0.001 μg / mL .

[0020] (2) The EC of the mixture of two pesticides against rice sheath blight 50 value determination

[0021] Take an appropriate amount of acetone to dissolve the original drug of phen...

Embodiment 2

[0031] Example 2 The in vitro control effect of the compound of the present invention on rice sheath blight

[0032] Weigh phenazine-1-carboxamide and fluazinam original drug with a balance, dissolve with appropriate amount of acetone, dilute with sterile water, and prepare 500 μg / mL mother solution for future use. In this experiment, there were 4 treatments and 1 water control, namely phenazine-1-carboxamide treatment (32 μg / mL), fluazinam treatment (0.14 μg / mL), phenazine-1-carboxamide and fluazinam Compound treatment (0.15 μg / mL), control drug Jinggangmycin treatment (conventional recommended drug concentration) and water control treatment. Spread the prepared medicinal solution evenly on the rice leaf sheaths, dry and inoculate rice sheath blight bacteria, repeat each treatment 3 times, use sterile water as a control, and place it in a dark incubator at 20-23°C for continuous Cultivate 72h, adopt cross method to measure bacterium colony diameter, calculate control effect,...

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Abstract

A synergistic composition used for rice sheath blight control and application thereof are disclosed. Phenazine-1-carboxamide and fluazinam which are two agents are measured by a mycelial growth rate process, and the synergistic potential of the composition for rice sheath blight control is evaluated by adopting a Wadley method. Results prove that the phenazine-1-carboxamide and fluazinam can suppress rice sheath blight. After composition of the two agents, the composition of the two agents in a ratio of 3:7 or 1:1 has synergistic effect on rice sheath blight, with the synergistic effect beingoptimum when the ratio is 1:1 and the synergistic coefficient SR being 1.85. An in-vitro control result shows that the control effect of the composition of the phenazine-1-carboxamide and fluazinam ina ratio of 1:1 is 82.89%, and the composition can be adopted as a novel agent for rice sheath blight control.

Description

technical field [0001] The invention belongs to the field of pesticide compounding, and mainly relates to a synergistic compound composed of phenazine-1-carboxamide and fluazinam, and also relates to the application of the compound in preventing and treating rice sheath blight. Background technique [0002] Rice is an important food crop, and its safe production affects the long-term stability of the country. Rice production is often disturbed by many factors, such as temperature, light, nutrition, pests and weeds, among which rice sheath blight is one of the important factors leading to rice yield reduction. The disease is a fungal disease caused by Rhizoctonia solani. It mainly infects the base leaf sheaths of rice at the end of tillering and the end of full ear, forming "moiré" lesions, and the annual yield loss caused by the disease 10-30%, severe up to 50%. [0003] In terms of production, the methods for controlling rice sheath blight mainly include agricultural cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/60A01N43/40A01P3/00
CPCA01N43/60A01N43/40
Inventor 张亚廖晓兰刘青王翀刘双清
Owner HUNAN AGRICULTURAL UNIV
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