Kit for detecting COX-2 gene polymorphism

Abstract

The invention discloses a kit for detecting COX-2 gene polymorphism. The kit comprises a box body, a box cover, a sponge lining, a freezing box frame and a freezing box, wherein the box cover is connected with the box body through hinging; the box body is internally provided with a first groove; the freezing box frame is vertically arranged at the middle position of the first groove; and the sponge lining is located in the box body. The kit disclosed by the invention is applicable to clinical detection of various sample types and has the advantages of strong specificity, high sensitivity, short experiment period, low cost and the like; the wicked problems in a transportation process of the kit are solved, and the kit can be rapidly taken and is simple and convenient to operate in a testingprocess; the box body of the kit adopts a three-layered structure; an inner layer is made of PS (Polystyrene) and has the advantages of safety and no toxicity, high temperature resistance and easiness for washing; a heat-insulation layer is made of PU (Polyurethane), has a low-temperature heat conduction function, can keep temperature and humidity balance for long time and has vibration-proof andcompression-resisting, waterproof and moisture-proof functions; and an outer layer is made of PE (Polyethylene) and has the advantages of strong chemical stability, safety and environment friendlessand good wear resistance.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a kit for detecting COX-2 gene polymorphism. Background technique [0002] Cyclooxygenase is the key enzyme for the synthesis of various prostaglandins (PGs). The phospholipids in the cell membrane release arachidonic acid under the catalysis of phospholipase A2, and the latter forms PGH2 under the catalysis of cyclooxygenase, and PGH2 is released in the corresponding PGE, PGF, PGD, PGI, etc. are synthesized under the action of prostaglandin isomerase. COX has two isoenzymes, COX-1 and COX-2. The human COX-2 gene is located in the 1q25.2-q25.3 region of the chromosome. It is about 8.3kb in length, contains 10 exons and 9 introns, and encodes a total of 604 amino acids. COX-2 is an inducible enzyme. Under physiological conditions, it cannot be detected in most tissues and cells. In vitro, many stimuli, such as growth factors, inflammatory cytokines, tumor inducers, etc...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kit for detecting COX-2 gene polymorphism, which solves the problems of high cost, time-consuming, low specificity and complicated operation for the detection of COX-2 gene polymorphism in the current market, The kit is small in size, easy to operate, easy to transport, carry and use

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