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Cochliobolus quinone B derivative, production strain and applications

A technology of Columella spiralis and derivatives, which is applied in the field of new compounds of Columella spiralis and natural compounds, can solve the problems of long growth cycle, limited resources, and no literature reports, etc., and achieves low production cost and convenient use. Effect

Inactive Publication Date: 2018-12-07
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to limited resources, long growth cycle and environmental protection, its development and utilization have been greatly restricted.
At present, the research on Salvia miltiorrhiza focuses on the chemical components and pharmacological effects, and the attention to its endophytic fungi has gradually increased; however, the separation of the secondary metabolites of its endophytic fungi has not been reported in the literature.

Method used

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  • Cochliobolus quinone B derivative, production strain and applications
  • Cochliobolus quinone B derivative, production strain and applications
  • Cochliobolus quinone B derivative, production strain and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Isolation and Characterization of Strain KLBMPSM007:

[0035] The samples of Salvia miltiorrhiza from Pizhou, Jiangsu and Rushan, Shandong were collected, and the roots, stems, leaves, and flowers were sorted and classified. After the samples were sterilized on the surface, they were cut into 0.5×0.5cm blocks and placed in separate cultures. basically. Observe the growth of the colonies on time every day. When fungal colonies grow out, transfer the fungi growing on the edge of the tissue block to fresh medium as soon as possible, purify 2 to 3 times, and obtain the fungus KLBMPSM007. The colony morphology of the strain KLBMPSM007 on the solid plate is: grayish white, fluffy, slightly raised in the middle. The strain has no flagella, is Gram-positive, and is resistant to streptomycin and penicillin.

[0036] The full sequence of the ITS region of the bacterial strain KLBMPSM007 was amplified and sequenced, and the complete sequence of the rDNA ITS sequence obtained by ...

Embodiment 2

[0040] Strain fermentation and product isolation and purification

[0041]seed medium

[0042] PDB rich medium: potato 200g, glucose 20g, KH 2 PO 4 1g, MgSO 4 0.5g, 1L of double distilled water, natural pH, sterilized at 115°C for 30min.

[0043] Fermentation medium

[0044] Rice solid medium: put 30g of rice in a culture bottle, add nutrient solution, each liter of nutrient solution contains 1L of double distilled water, KH 2 PO 4 1.0g, MgSO 4 0.5g, mix thoroughly and soak for about 4 hours, and sterilize at 121°C for 30 minutes.

[0045] The specific process of strain fermentation:

[0046] Transfer the strain from the slant stored at low temperature to a new PDA plate for activation, and cultivate it in a constant temperature incubator at 28°C for 3-5 days;

[0047] Pick a small amount of mycelium and inoculate it in the PDB-enriched medium, and shake it on a constant temperature shaker at 28°C and 120r / min for 48h to prepare the seed solution;

[0048] Inoculate ...

Embodiment 3

[0062] Anti-Pseudomonas aeruginosa Infection Experiment of the New Compound Cyclosporin

[0063] 1. CoB1 significantly promotes the survival rate of Pseudomonas aeruginosa-infected mice

[0064] Inject CoB1 into C57BL / 6J mice by tail vein injection (100g / 100 L PBS per mouse, once every 24 hours, twice in total) to construct the mouse model of CoB1 treatment group. At the same time, an equal volume of 100 L PBS was injected mice served as the control group. After the model was established successfully, Pseudomonas aeruginosa PAO1 (1×107 / monkey, 10 in each group) was perfused into the nasal cavity, observed continuously for 7 days, and the growth curve was drawn. The results showed that CoB1 significantly increased the survival rate of Pseudomonas aeruginosa-infected mice (attached Figure 8 ).

[0065] 2. CoB1 can significantly inhibit the diffusion rate of Pseudomonas aeruginosa in the lungs of mice. Nasal perfusion of Pseudomonas aeruginosa PAO1 genetically engineered stra...

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Abstract

The invention discloses a cochliobolus quinone B derivative, a production strain and applications. The structural formula of the derivative is shown as the description. The derivative is produced by aBipolaris sorokiniana KLBMPSM007 obtained through separating and screening; and the Bipolaris sorokiniana KLBMPSM007 has been deposited in a common microorganism center of the China committee for culture collection of microorganisms, the deposited time is on 5th June, 2017, and the deposited number is CGMCC NO. 13900. The compound can significantly increase the survival rates of mice infected bypseudomonas aeruginosa, reduce lung injury and bacterial diffusion, and show good biological effects of against pseudomonas aeruginosa infection; and the occurrence of autophagy of alveolar macrophagecan be directly induced through compound processing, so that the macrophage can be promoted to perform identification, phagocytosis and degradation on the pseudomonas aeruginosa, and the invading ofthe pseudomonas aeruginosa can be resisted.

Description

technical field [0001] The present invention relates to the fields of microbiology, organic chemistry and medicinal chemistry, and in particular to a new natural compound, a new compound of Cyclosporium quinone isolated from an endophytic fungus of Salvia miltiorrhiza, Helminthia solani, and a preparation method of the compound and apply. . Background technique [0002] As an important model medicinal plant, Salvia miltiorrhiza has multiple values ​​such as medicine, food, scientific research and economy, and has various pharmacological activities such as myocardial protection, immune regulation, anti-tumor, anti-infection and anti-arrhythmia. The part used as medicine is the root. Due to limited resources, long growth cycle and environmental protection, its development and utilization have been greatly restricted. At present, the research on Salvia miltiorrhiza mainly focuses on the chemical components and pharmacological effects, and the attention to its endophytic fung...

Claims

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Application Information

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IPC IPC(8): C07D493/04C12N1/14C12P17/18A61P31/04C12R1/645
CPCA61P31/04C07D493/04C12P17/181C12N1/145C12R2001/645
Inventor 蒋继宏袁博丁盼李荣鹏刘金娟李丹朱鹏程
Owner XUZHOU NORMAL UNIVERSITY
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