Concentration response type biochemical multi-component online analyzer

Abstract

The invention discloses a concentration response type biochemical multi-component online analyzer. The concentration response type biochemical multi-component online analyzer comprises a multi-component concentration detection module, a precise sampling mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure; the multi-componentconcentration detection module is composed of a detection pool, a sample pool, a sampling pool, a cleaning pool and a waste liquid pool, and can realize online detection on multi-component concentration; the sampling mechanical arm is composed of a two-freedom-degree mechanism arm, a precision syringe and a sample injection needle, and realizes precise locating sampling control on the detection pool; the multi-channel data acquisition module is composed of an embedded development board and a touch screen, and realizes online detection, display and storage on the multi-component concentration;the automatic sampling module is composed of a precision membrane sampler, a miniature vacuum diaphragm pump and a stop valve; and the four parts are linked and assembled through a multi-channel flowpath integrated structure. According to the concentration response type biochemical multi-component online analyzer disclosed by the invention, online real-time detection on multiple substrates, keyintermediate metabolites and product concentrations in a microbial fermentation process can be realized; the structure is compact; and batch production is conveniently carried out.

Description

technical field [0001] The invention belongs to the interdisciplinary field of biosensing technology and industrial automation technology, and specifically relates to an on-line multi-component concentration analyzer for microbial fermentation processes based on a biosensing electrode and capable of responding to various component concentrations. Background technique [0002] The fermentation process mainly involves the growth and metabolism of microorganisms. The substrates, key intermediate metabolites, product types and concentrations and interactions in the fermentation system will all affect the reaction rate, product yield and cell-to-metabolism pathways in the microbial fermentation process. choice has a significant impact. The purpose of regulating the fermentation process is to adjust the environment and other factors to the most suitable state for the growth of microorganisms. During the regulation process, it is necessary to understand the relevant information of ...

AI Technical Summary

Problems solved by technology

Most of the component concentration analysis instruments currently on the market use off-line sampling, dilution requires manual sampling and complex pretreatment for off-line detection of low-concentration component concentrations, while the component concentrations in fermentation systems are often much higher Due to the detection linear range of these instruments, it is necessary to dilute the sample for offline detection, which greatly prolongs the detection cycle, and the detection data cannot reflect the concentration changes of components in the current fermentation system in time (such as substrates, key intermediate metabolism, etc.) substances, products), so it cannot give timely guidance and regulation to the fermentation process, which greatly limits the wide application of fermentation process control technology based on concentration feedback

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