On-machine serum diluent for chemiluminescence instrument and preparation method of on-machine serum diluent

Abstract

The invention discloses an on-machine serum diluent for a chemiluminescence instrument and a preparation method of the on-machine serum diluent. The on-machine serum diluent contains the following components in parts by weight: 21-45 parts of dipotassium phosphate, 22-50 parts of potassium dihydrogen phosphate, 11-22 parts of sodium hydrogen carbonate, 11-19 parts of zinc sulfate, 15-31 parts of heparin sodium, 7-17 parts of sorbitol, 3-11 parts of glycine, 3-7 parts of polyoxyethylene, 4-9 parts of polyethylene glycol, 1-4 parts of nonylphenol polyoxyethylene (10) ether, 0.1-0.4 part of casein sodium, 0.2-0.6 part of gentamicin, 0.1-0.4 part of sodium azide and 80-180 parts of deionized water. The on-machine serum diluent for the chemiluminescence instrument is prepared by virtue of the synergistic effect among the components, can be used for effectively diluting a serum sample and can be rapidly and uniformly mixed with serum, a detection result is not interfered, and the nonspecificadsorption can be reduced.

Description

technical field [0001] The invention relates to the technical field of immune reagents, in particular to an on-board serum diluent for a chemiluminescent instrument and a preparation method thereof. Background technique [0002] With the rapid development of biology, the cognition of the human body is becoming more and more in-depth and refined, and the detection of biological macromolecules such as proteins and accounting is increasing, and its technical means are constantly innovating and improving. Among them, immunology is a particularly prominent branch of modern medical biological research, which is based on the specific combination of antigens and antibodies. Based on the specific combination of antigen and antibody, it was first used in radioimmunoassay technology, and then enzyme immunoassay technology was derived because of its pollution problem. Although enzyme immunology technology solved the pollution problem well, its sensitivity could not reach that of radioim...

AI Technical Summary

Problems solved by technology

Based on the specific combination of antigen and antibody, it was first used in radioimmunoassay technology, and then enzyme immunoassay technology was derived because of its pollution problem. Although enzyme immunology technology solved the pollution problem well, its sensitivity could not reach that of radioimmunoassay. Height, and then the birth of chemiluminescent immune technology has solved these two problems

However, when the specific binding reaction between the antigen and the antibody is used for detection, false positives or false negatives will occur. This problem not only brings harm to patients but also causes corresponding losses to hospitals; The detection result is low because the concentration of the marker to be tested is too high and the amount of the captured marker is insufficient, or the result of the false negative result is that the concentration of the marker to be tested is too high and the reaction fails to control the compressed space.