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Method for producing xylo-oligosaccharide

A technology of xylo-oligosaccharide and manufacturing method, applied in the directions of sugar production, biochemical equipment and method, glycosylase, etc., to achieve the effect of a simple method

Active Publication Date: 2018-11-23
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, since xylanase is produced as an enzyme component of a cellulase composition produced by microorganisms, in the production method of an enzyme using xylanase, the enzyme whose main component is xylanase Manufacturing cost becomes an issue

Method used

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  • Method for producing xylo-oligosaccharide

Examples

Experimental program
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Embodiment

[0087] Hereinafter, the present invention will be specifically described with reference to Examples and Comparative Examples.

reference example 1

[0089] Pretreatment of biomass containing xylan and cellulose

[0090] Bagasse was used as biomass containing xylan and cellulose for pretreatment. Measure 5 g of bagasse, and heat until it becomes 105°C. Based on the weight change at this time, the solid content rate of bagasse was calculated. The value obtained by multiplying the bagasse in a water-containing state by the water content was defined as the solid content weight. 100 g of bagasse is immersed in an aqueous sodium hydroxide solution in such a manner that the weight of the solid content is 5% by weight in a state mixed with the sodium hydroxide solution, and the amount of sodium hydroxide added to the solid content of the bagasse is 10% by weight. Pretreatment at 80°C for 3 hours. Solid-liquid separation was performed by centrifugation (3,000G, 10 minutes), and it isolate|separated into a solution component and a solid component. The solid content washed with pure water was used in the following experiments as ...

reference example 2

[0092] Modulation of cellulase composition

[0093] [former culture]

[0094] To become corn steep liquor (Corn Steep Liquor) 5% (w / vol), glucose 2% (w / vol), ammonium tartrate 0.37% (w / vol), ammonium sulfate 0.14 (w / vol), potassium dihydrogen phosphate 0.2 % (w / vol), calcium chloride dihydrate 0.03% (w / vol), magnesium sulfate heptahydrate 0.03% (w / vol), zinc chloride 0.02% (w / vol), iron(III) chloride ) hexahydrate 0.01% (w / vol), copper (II) sulfate pentahydrate 0.004% (w / vol), manganese chloride tetrahydrate 0.0008% (w / vol), boric acid 0.0006% (w / vol) , Hexaammonium heptamolybdate tetrahydrate 0.0026% (w / vol) were added to distilled water, 100mL was poured into a 500mL Erlenmeyer flask with a baffle, and autoclaved at 121°C for 15 minutes Sterilization of the sterilizer. After cooling, 0.01% (w / vol) of PE-M and Tween80 which were separately autoclaved at 121° C. for 15 minutes were added to prepare preculture media. In the pre-culture medium 100mL to become 1×10 5 Trichod...

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Abstract

Disclosed is a method for producing a xylo-oligosaccharide from a biomass containing xylan and cellulose, wherein the method is simple and a high xylo-oligosaccharide yield is achieved by suppressingxylo-oligosaccharide from being broken down to xylose. According to the method for producing xylo-oligosaccharide, a biomass containing xylan and cellulose is hydrolyzed by a cellulase composition that, at the time of hydrolysis, at least exhibits xylanase, cellobiohydrolase, and beta-glucosidase activities, but does not substantially exhibit beta-xylosidase activity.

Description

technical field [0001] The present invention relates to a method for producing xylo-oligosaccharides from biomass comprising xylan and cellulose. Background technique [0002] Xylooligosaccharides are a general term for oligosaccharides obtained by combining multiple xylose units through β-glycosidic bonds. Xylo-oligosaccharides are also used as materials for functional foods because they exhibit an excellent gut-regulating action (Non-Patent Document 1). [0003] Xylo-oligosaccharides can be obtained by hydrolyzing xylan contained in biomass containing xylan and cellulose. As methods of hydrolysis, a method of hydrothermal treatment (Non-Patent Document 2), a method of acid hydrolysis (Non-Patent Document 3), and a method of enzymatic treatment with xylanase (Patent Document 1) are known. [0004] Among them, when xylan is hydrolyzed with xylanase, xylo-oligosaccharides can be selectively produced, so xylo-oligosaccharides can be produced efficiently. However, xylan, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C13K13/00C12N9/42
CPCC12P19/12C12P19/14C13K13/00C12P19/04C12N9/2445C12N9/2482C12Y302/01021C12N9/2485C12N9/2437C12Y302/01091C12Y302/01008C12Y302/01037
Inventor 笠原拓也山田千晶栗原宏征山田胜成
Owner TORAY IND INC
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