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Egg yolk phosphatidyl choline preparation method based on low-pressure column chromatography

A technology of egg yolk phosphatidylcholine and phosphatidylcholine, which is applied to edible phospholipid compositions, chemical instruments and methods, protein food ingredients, etc., can solve problems such as high equipment cost, small processing capacity, and heavy metal ion residues, and achieve The effect of simple operation process, high production efficiency and low cost

Inactive Publication Date: 2018-11-23
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the metal ion precipitation method will cause the problem of heavy metal ion residue; high performance liquid chromatography and supercritical fluid extraction method have good separation effect and high product quality, but there are problems of high equipment cost and limited large-scale industrial application; membrane separation method has Membrane cleaning is difficult and the processing volume is small

Method used

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  • Egg yolk phosphatidyl choline preparation method based on low-pressure column chromatography

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] S1. Filler activation: Weigh 15g of mixed silica gel powder with a mesh number of 200-300, and activate it in an oven at 50°C for 6 hours;

[0034] S2, column packing: choose a glass chromatography column with a length of 200 mm and a diameter of 16 mm to separate and purify phosphatidylcholine (PC), add 30 mL of chloroform to the activated silica gel powder in step S1 and stir it into a slurry, and use a wet method The column packing method puts silica gel into a glass chromatography column to obtain a silica gel column;

[0035] S3. Dissolve 200 mg of egg yolk crude phospholipids in 1 mL of chloroform and slowly add them into the silica gel chromatography column prepared in step S2 along the column wall;

[0036] S4. Gradient elution: use 20 times the column volume of chloroform, methanol, and glacial acetic acid ternary mixed solvent (chloroform:methanol:glacial acetic acid=18:5:1, volume ratio) for the first elution, remove the After the pigment and other phospholi...

Embodiment 2

[0040] S1. Filler activation: Weigh 15g of silica gel powder with a mesh number of 200-300, and activate it in an oven at 55°C for 5h;

[0041] S2, column packing: choose a glass chromatography column with a length of 200 mm and a diameter of 16 mm to separate and purify phosphatidylcholine (PC), add 35 mL of chloroform to the activated silica gel powder in step S1 and stir it into a slurry, and use a wet method The column packing method puts silica gel into a glass chromatography column to obtain a silica gel column;

[0042] S3. Dissolving 150 mg of egg yolk crude phospholipids in 1 mL of chloroform and slowly adding to the silica gel chromatography column prepared in step S2 along the column wall;

[0043]S4. Gradient elution: first elute with 15 times the column volume of chloroform, methanol, and glacial acetic acid ternary mixed solvent (chloroform:methanol:glacial acetic acid=17:5:1, volume ratio) for the first elution, remove the After the pigment and other phospholip...

Embodiment 3

[0047] S1. Filler activation: Weigh 15g of silica gel powder with a mesh number of 200-300, and activate it in an oven at 60°C for 4 hours;

[0048] S2, column packing: choose a glass chromatography column with a length of 200 mm and a diameter of 16 mm to separate and purify phosphatidylcholine (PC), add 40 mL of chloroform to the activated silica gel powder in step S1 and stir it into a slurry, and use a wet method The column packing method puts silica gel into a glass chromatography column to obtain a silica gel column;

[0049] S3. Dissolve 300 mg of egg yolk crude phospholipids in 1 mL of chloroform and slowly add them into the silica gel chromatography column prepared in step S2 along the column wall;

[0050] S4. Gradient elution: use 10 times the column volume of chloroform, methanol, and glacial acetic acid ternary mixed solvent (chloroform:methanol:glacial acetic acid=18:5:1, volume ratio) for the first elution, remove the After the pigment and other phospholipid co...

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Abstract

The invention belongs to the technical field of phospholipid deep processing, and relates to an egg yolk phosphatidyl choline preparation method based on low-pressure column chromatography. Egg yolk raw phospholipid is dissolved in chloroform; silica gel is used as a stationary phase; a chloroform, methyl alcohol and glacial acetic acid mixed solution is used as an eluting agent for performing gradient elution; in the elution process, the thin-layer chromatography is used for monitoring; phosphatidyl choline containing ingredients are collected and eluted; rotary evaporation and concentrationare performed; a termovap sample concentrator is used for drying; egg yolk phosphatidyl choline with the purity being 90 to 98 percent is prepared. A ternary mixed solvent is used for gradient elution; the operation process is simple; the process is simple; complicated equipment is not needed; the cost is low; the production efficiency is high; the method is suitable for industrial production; organic solvents can be recovered and used; fillers are repeatedly used through regeneration treatment. The prepared egg yolk phosphatidyl choline can be used in the industrial fields of functional food,medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to the technical field of phospholipid deep processing, in particular to a method for preparing egg yolk phosphatidylcholine based on low pressure chromatography. Background technique [0002] Phosphatidylcholine (PC), also known as lecithin, is the main component of phospholipids. There are two isomers of PC, α and β, according to the connection position of phosphate and glycerol, and natural PC is dominated by α. As the main component of biofilm, PC plays a key role in biochemistry and physiology, such as: improving memory, reducing stress, enhancing brain function of infants, promoting nerve conduction, relieving anxiety, etc. Phosphatidylcholine widely exists in biological tissues such as soybeans and egg yolks. In view of soybean transgenic issues and soybean protein allergy issues, egg yolk can be used as a preferred raw material for the preparation of phosphatidylcholine. Phosphatidylcholine in egg yolk The content accoun...

Claims

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Application Information

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IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 孙娜陈晋林松毅鲍志杰李冬梅沈佳颖
Owner DALIAN POLYTECHNIC UNIVERSITY
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