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Method for detecting ancient wool fabric fragments based on immunoblotting

An immunoblotting method and wool fabric technology, which is applied in the field of cultural relics detection, can solve the problems of wool cultural relics identification and protection research difficulties, the identification results are not fully accurate, and the analysis of fiber morphology is not enough, and the size can be adjusted and accurately The effect of high precision and high sensitivity

Active Publication Date: 2018-11-16
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, it is not difficult to imagine that in the long history, woolen fabrics buried in tombs or ruins have already lost their original appearance and degraded into Peptides or amino acids cannot be recognized by the naked eye, and the older the evidence, the harder it is to find, which brings great difficulties to the identification and protection of wool cultural relics
[0004] At present, ordinary analysis and detection techniques, such as Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction analysis, etc., are often at a loss when encountering such residue analysis. Most of the identification of ancient woolen fabrics at home and abroad still remain In the analysis of fiber morphology, the identification results obtained are not completely accurate; therefore, it is very necessary to find a more scientific method to identify ancient woolen fabrics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 20:1 to 20 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 50%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 2:1, shake vigorously to obtain an inverse microemulsion, and let it stand for 12 hours; Add 0.3 g of dodecylamine to the microemulsion, ultrasonicate for 10 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 120 °C for 3 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed with a dialysis bag with a molecular weight cut off of 1000 for 24 h, and then freeze-dried to obtain car...

Embodiment 2

[0066] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 18:1 to 15 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 45%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 1.8:1, and shake vigorously to obtain an inverse microemulsion, which is left to stand for 10 h; Add 0.2 g of dodecylamine to the microemulsion, ultrasonicate it for 8 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 110 °C for 2 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed for 20 h with a dialysis bag with a molecular weight cut off of 1000, and freeze-dried to obtai...

Embodiment 3

[0080] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 22:1 to 25 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 55%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 2.2:1, shake vigorously to obtain a reverse-phase microemulsion, and let it stand for 13 hours; Add 0.4 g of dodecylamine to the microemulsion, sonicate for 12 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 130 °C for 4 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed for 28 hours with a dialysis bag with a molecular weight cut off of 1000, and freeze-dried to obtain ca...

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Abstract

The invention discloses a method for detecting ancient wool fabric fragments based on immunoblotting, belonging to the field of cultural relic detection. The method of the invention comprises the following steps: preparing a carbon quantum dot-labeled wool secondary antibody at first, then carrying out extraction on modern wool and ancient wool fabric fragments by using a metal salt process and areduction process, carrying out SDS-PAGE gel electrophoresis, then carrying out staining with a carbon quantum dot solution, and observing an electrophoresis image; and transferring obtained protein bands to a polyvinylidene fluoride membrane, carrying out incubation with a wool primary antibody and the carbon quantum dot-labeled secondary antibody, and observing immunological bands in a gel imaging system so as to identify an ancient wool fabric. According to the invention, the method is low in the usage amount of chemical reagents, mild in reactions, friendly to environment and free of harm;and when applied to the detection of ancient wool fabrics, the method has the advantages of low usage amount of samples, sensitivity, accuracy, visuality and the like.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient wool fabric fragments based on the immunoblotting method. Background technique [0002] China has a long history. A large number of wool products have been unearthed from early Chinese tombs. The quantity and the exquisite weaving are both amazing. The study of these unearthed wool fabrics plays a decisive role in understanding the origin of ancient wool fabrics, ethnic minority costumes, and inter-ethnic cultural exchanges. [0003] Among the remains of ancient wool fabrics, wool remains are mainly wool fabric fragments. Such remains have been buried in the underground environment for a long time. Under the influence of environmental factors such as water, heat, oxygen, and microorganisms, wool keratin will undergo denaturation and aging degradation. After being unearthed, due to drastic changes in environmental conditions, the aging ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 王秉王钟元胡铭周周莲彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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