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Photosensitive type cell membrane biomimetic targeting nano-drug for tumor combined therapy and preparation thereof

A technology of combined therapy and nano-drugs, which is applied in the direction of anti-tumor drugs, drug combinations, and medical preparations of non-active ingredients, etc., can solve the problems of easy aggregation, photostability, limited application, and poor quality of the solution, and achieve improvement in the body. Short circulation time, prolong circulation time in the body, and control the distribution in tissues and cells

Active Publication Date: 2018-11-16
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the short half-life of indocyanine green, it is easy to specifically bind to lipoproteins in the body, easy to aggregate in solution and poor in photostability, resulting in self-quenching of fluorescence, which limits its application in the medical field.

Method used

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  • Photosensitive type cell membrane biomimetic targeting nano-drug for tumor combined therapy and preparation thereof
  • Photosensitive type cell membrane biomimetic targeting nano-drug for tumor combined therapy and preparation thereof
  • Photosensitive type cell membrane biomimetic targeting nano-drug for tumor combined therapy and preparation thereof

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Embodiment 1

[0050] (1) Preparation of protein drug carrier: at 25°C, first dissolve 50 mg bovine serum albumin (BSA, purchased from sigma company) in a phosphate buffer solution with pH=9, and then add dropwise 10 ml containing 45 mg 1,4-dioxane of succinic anhydride is used to modify the surface of bovine serum albumin, so that the surface can be grafted with carboxyl groups as much as possible, and react for 2 hours at room temperature. After the reaction, the resulting reaction solution is filtered, and the resulting filtrate 4 ℃ placed in an ultrafiltration centrifuge tube for centrifugation and ultrafiltration, the molecular weight cut-off of the ultrafiltration membrane is 10000KDa, the centrifugation speed is 4000rpm, and the centrifugation time is 20min; the liquid in the ultrafiltration tube is freeze-dried to obtain carboxyl-modified albumin.

[0051] (2) Take 15 mg of lyophilized carboxy-modified albumin prepared in step (1), add 28 mg of DTT and 6 mg of indocyanine green (purch...

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Abstract

The invention belongs to the technical field of nano-medicines, and discloses a photosensitive type cell membrane biomimetic targeting nanodrug for tumor combined therapy and a preparation method thereof. According to the nanodrug and the preparation method thereof, a human O-type red blood cell membrane is used as a carrying platform, albumin is used as a drug carrier, DACHPt is used as a chemotherapeutic drug, ICG is used as a photosensitive reagent, RGD is used as a targeting molecule, so that a biomimetic drug carrying system with biomimetic characteristic and high drug loading capacity and capable of being specifically targeted at tumor cells and realizing synergistic anti-tumor sensitization is prepared, the novel biomimetic drug carrying system not only can realize efficient carrying of the drug, but also can effectively prolong the in-vivo circulation time, so that accurate and continuous drug administration at the position of a focus of a tumor is realized, the defect of tumordrug administration can be efficiently overcome through the biomimetic effect of the drug, and a drug carrying and administration and targeted therapy synergistic working mechanism is formed; and thepurpose of tumor chemotherapy and photo-thermal therapy multi-mechanism combined tumor treatment is achieved, and a new idea and platform are provided for tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a photosensitive cell membrane bionic targeted nanomedicine for combined treatment of tumors and a preparation method thereof. Background technique [0002] Red blood cells (RBCs) are the most numerous and longest-lived cells in the blood, have high biocompatibility, circulate in the body for as long as 120 days, and can be completely degraded. RBCs have many unique properties, such as a wide range of circulation, automatic clearance of aged and damaged RBCs by the reticuloendothelial system, long half-life cycle, and optimal surface area to volume ratio, making them ideal candidates for many natural or synthetic payloads. candidates for in vivo delivery. Red blood cell membrane carriers have received extensive attention in the past ten years. As the most commonly used active reagent carriers in blood, they are often called "red blood cell carriers" or "RBCs car...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/555A61K47/42A61K47/46A61P35/00
CPCA61K31/555A61K41/0057A61K47/42A61K47/46A61P35/00A61K2300/00
Inventor 戴箭刘雯薛巍
Owner JINAN UNIVERSITY
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