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A method for determining the content of melamine in dairy products

A technology for melamine and dairy products, which is applied in the field of determining the content of triazine nitrogen-containing heterocyclic organic compounds in food, can solve the problems of complicated steps, long time-consuming, high detection cost by liquid chromatography-mass spectrometry and the like, and achieves high sensitivity, low cost effect

Active Publication Date: 2021-05-18
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation and processing steps of the detection process are complicated, time-consuming, and the amount of reagents and consumables is large, which is not conducive to the rapid detection of multiple samples of dairy products in real life
my country's current national standard GB / T22388-2008 "Detection method for melamine in raw milk and dairy products" stipulates high-performance liquid chromatography, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry detection, of which high-performance liquid chromatography Low sensitivity, high detection false negative rate, gas chromatography-mass spectrometry sample processing steps are complex, not suitable for various types of samples, liquid chromatography-mass spectrometry detection cost is high, so the popularization and application in various places is still very limited

Method used

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  • A method for determining the content of melamine in dairy products
  • A method for determining the content of melamine in dairy products
  • A method for determining the content of melamine in dairy products

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1 (measurement method example)

[0029] 1. Instruments, reagents and samples

[0030] 1.1. Instruments: Vortex-Genie adjustable vortex mixer; XMTD-204 constant temperature water bath; micropipette; MiLLi-Q ELement ultrapure water treatment system; 1 / 1000 electronic balance; G13U desktop centrifuge; LC-20A High performance liquid chromatography (SPD-M20A diode array detector); Shim-Pack VP-ODS column chromatography column (250×4.6mm, 5μm).

[0031] 1.2. Reagents: Triton X-114; NaCl (chromatographically pure); formic acid (chromatographically pure); sodium octane sulfonate (chromatographically pure); citric acid; acetonitrile (chromatographically pure); methanol (chromatographically pure).

[0032] Prepare 200μL / mL Triton X-114 solution: Take a 10mL volumetric flask and add 2mL Triton X-114, dilute to the mark with ultrapure water, shake well, put it in a -4°C refrigerator for 4 hours, take it out and return to room temperature for later use .

[0033] Prepare ...

Embodiment 2

[0065] Embodiment 2 (kit product example)

[0066] The kit of this embodiment includes reagent A, reagent B, reagent C, reagent D and reagent E, and the preparation method of each reagent is as follows.

[0067] Reagent A: 200μL / mL Triton X-114 solution; the preparation method is as follows: take 2mL Triton X-114 and dilute to 10mL with water;

[0068] Reagent B: 2mg / mL NaCl solution; the preparation method is as follows: take 40mg NaCl and dilute to 20mL with water;

[0069] Reagent C: 24 μL / mL formic acid solution; the preparation method is as follows: take 240 μL formic acid and dilute to 10 mL with water;

[0070] Reagent D: melamine standard solid 2.0 mg;

[0071] Reagent E: formic acid solution with a volume concentration of 0.6%; the preparation method is as follows: take 300 μL of formic acid and dilute to 50 mL with water;

[0072] Reagent F: sodium octane sulfonate-citric acid aqueous solution, the sodium octane sulfonate-citric acid aqueous solution is made by th...

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Abstract

The present invention relates to testing or analyzing materials by means of measuring the chemical or physical properties of materials, in particular to a method for measuring the content of melamine in dairy products, the method comprising the following steps: taking dairy products, adding Triton X-114 aqueous solution, NaCl aqueous solution and formic acid, so that the final concentration of Triton X-114 is 30-50 μL / mL, the final concentration of NaCl is 0.4-1.2 mg / mL, and the final concentration of formic acid is 2.0-8.0 μL / mL; shaking, 40 ° C constant temperature water bath, Centrifuge, take the supernatant, filter to obtain the sample injection solution of the sample to be tested; configure the melamine standard stock solution, dilute the stock solution with a formic acid aqueous solution with a volume concentration of 0.6%, and obtain a series of concentration standard sample injection solutions; The sample injection solution of the sample to be tested and a series of concentration standard sample injection solutions are detected by liquid phase ion pair chromatography, and the liquid chromatograms of the sample to be tested and the standard are obtained, and then the two spectra are compared to determine the characteristics Peak, then calculate the content of melamine in the detection sample with the accompanying standard curve according to the peak area.

Description

technical field [0001] The invention relates to testing or analyzing materials by means of measuring their chemical or physical properties, in particular to a method for measuring the content of triazine nitrogen-containing heterocyclic organic compounds in food. Background technique [0002] Melamine, commonly known as melamine and protein essence, is a synthetic triazine nitrogen-containing heterocyclic organic compound, which is widely used in the chemical industry. Melamine has chronic toxicity to the human body. Studies have shown that long-term intake of melamine in animals can cause urinary system damage, bladder and kidney stones, and can further cause transitional cell proliferation and induce bladder cancer. The incidence of stones in newborn animals is higher than that in adult animals. Much higher, so the health damage to infants and young children is particularly serious. In recent years, there have been reports of unscrupulous merchants illegally adding melami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 潘加亮袁嘉豪余洁纯黄建豪马安德
Owner SOUTHERN MEDICAL UNIVERSITY
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