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Multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof

A technology of soil phosphorus and Arthrobacter, which is applied in the direction of soil conditioning materials, applications, and microbial-based methods. It can solve the problems of few microbial research results, reduce the number of pathogenic bacteria, prevent disease outbreaks, and reduce the number.

Active Publication Date: 2018-11-13
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the strains with phosphorus removal effect are selected from sewage or sludge, and are concentrated in the prevention and control of polluted water bodies. There are few research results on microorganisms that control the loss of phosphorus at the source of pollution.

Method used

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  • Multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof
  • Multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof
  • Multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The screening of embodiment 1 bacterial strain

[0021] (1) Source of strain

[0022] The Arthrobacter Fp64 of the present invention is isolated and screened from the soil of a protected agricultural greenhouse, and the sampling depth is 20-40 cm.

[0023] (2) Primary screening

[0024] The primary screening of bacterial strains adopts PAOs screening medium, and the formula is: sodium citrate 4g, NaCl 0.5g, (NH 4 ) 2 SO 4 2.5g, CalCl 2 0.25g, MgSO 4 0.25g, Na 2 HPO 4 12.8g, KH 2 PO 4 3g, maltose 0.01g, toluidine blue 0.025g, agar 20g, no need to adjust pH, sterilize at 110°C for 20min. The culture temperature was 28°C.

[0025] The preliminary screening steps are as follows: (1) Prepare several test tubes containing 9ml water and several shake flasks containing 100ml water, autoclave at 121°C for 20 minutes, and cool down for later use; (2) Each 10g soil sample is filled into 100ml In the shaking flask of bacterial water, the shaker was shaken for 30 min...

Embodiment 2

[0031] Phosphorus removal effect and other characteristic identification of embodiment 2 joint bacillus Fp64

[0032] 1. Phosphorus removal effect

[0033]Arthrobacter Fp64 of the present invention and 8 bacterial strains (Bacillus subtilis Bp19, Rhodococcus rhodochrous Fp31, Microbacterium esteraromaticum Np20, Bacillus subtilis Bp15, Arthrobacter keyseri Fp38, Bacillus subtilis Bp13, Bacsubus methylp14, 2acillus trophicus Bp13) obtained by rescreening in Example 1 To compare the phosphorus removal rate, the experimental results are as follows figure 2 shown by figure 2 It can be seen that the phosphorus removal rate of Arthrobacter Fp64 in the present invention is 70.52% in a nutrient solution containing a final concentration of 10mg / L phosphorus. have significant advantages.

[0034] 2. Determination of salt tolerance

[0035] After the Arthrobacter Fp64 shaker of the present invention is activated, it is inoculated into LB liquids added with 50, 100 and 150 g / L NaCl ...

Embodiment 3

[0038] Soil adaptability of embodiment 3 Arthrobacter Fp64 and the effect measurement of reducing soil phosphorus loss

[0039] Cucumber seedlings were planted in a permeable pot with a diameter of 12 cm and a height of 30 cm. The potting soil was collected from the soil within 1 m of the greenhouse soil layer (the soil available phosphorus content was 49.55 mg / kg). The potting test was carried out immediately after the fresh soil was obtained. The treatment group was mixed with 20ml concentration of 1×10 8 / ml Arthrobacter Fp64 sterile water suspension soil, control group mixed with 20ml sterile water soil, five biological replicates were established. Measure the maximum water-holding capacity of the soil, water quantitatively and not exceed the water-holding capacity, and cultivate under the conditions of 25-28°C and 14-16h light.

[0040] In the treatment group and the control group, soil at a depth of 20 cm was collected at the time of inoculation and 30 days after cultiv...

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Abstract

The invention relates to multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof. The arthrobacter Fp64 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 26, 2018, and the preservation number is CGMCC No.15644. The arthrobacter Fp64 is used as a phosphorus regulating agent or the like in agricultural production, hasgood environmental adaptability and has the ability to resist pathogenic bacteria. When the arthrobacter Fp64 is added to soil, the loss of phosphorus in the soil can be effectively prevented.

Description

technical field [0001] The invention relates to a strain of multifunctional Arthrobacter Fp64 for controlling soil phosphorus loss and application thereof, belonging to the technical field of microbial application. Background technique [0002] Phosphorus is an essential nutrient element for agricultural crops. In actual production, a large amount of chemical fertilizers and organic fertilizers are often used to maintain production. The soil (especially the soil in the plow layer) is rich in phosphorus for a long time, and the accumulation of mobile forms of phosphorus in the soil is large, which is easy to Loss causes eutrophication of water bodies. It is estimated that about 3×10 6 ~4×10 6 tP 2 o 5 Migrate from soil to water body, and the amount of phosphorus entering water body from farmland in China is 19.5kg / hm 2 . When the loss of phosphorus in the agricultural environment reaches 0.015mg / L, it may cause the phenomenon of "algae bloom", which is the main limiting...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/06C09K101/00
CPCC09K17/14C09K2101/00C12N1/205C12R2001/06
Inventor 吴晓青张新建吕玉平周方园赵晓燕张广志任何周红姿谢雪迎
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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