Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of antioxidant active peptide and its application

An active peptide and anti-oxidation technology, which is applied in applications, medical preparations containing active ingredients, peptides, etc., can solve the problems of unfavorable active peptide efficacy, low bioavailability, and poor absorption of active peptides, and achieve high oral absorption efficiency , strong proliferative ability, and high antioxidant activity

Active Publication Date: 2021-03-19
HUAZHONG UNIV OF SCI & TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the oral absorption of active peptides is generally poor, and the bioavailability is low, which is not conducive to the efficacy of active peptides in vivo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of antioxidant active peptide and its application
  • A kind of antioxidant active peptide and its application
  • A kind of antioxidant active peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Determination of Antioxidative Ability of 12 Peptides by Hydroxyl Free Radical Scavenging Method

[0039] Prepare 1 mL of 1 mg / mL peptide solution, add 2 mL of 1.8 mM ferrous sulfate, 1.5 mL of 1.8 mM salicylic acid-ethanol solution, and 1 mL of 8.8 mM hydrogen peroxide solution in sequence, and incubate in a 37°C water bath for 30 min.

[0040] Prepare 1mL 1mg / mL walnut meal hydrolyzate solution, add 2mL 1.8mM ferrous sulfate, 1.5mL 1.8mM salicylic acid-ethanol solution, 1mL 8.8mM hydrogen peroxide solution, and incubate in a 37°C water bath for 30min.

[0041] Absorbance A1 was measured at 510 nm. Replace the sample with distilled water and detect according to the above method, and the measured absorbance is used as the control A0. Calculate the hydroxyl radical scavenging rate according to the following formula:

[0042] Hydroxyl radical scavenging rate (%)=[(A 0 -A 1 ) / A 0 ]×100%

[0043] The hydroxyl radical scavenging rate of 12 polypeptides is as follows: ...

experiment example 2

[0045] CCK-8 method to evaluate the effect of 12 peptides on the proliferation of oxidative damage to nerve cells

[0046] Construction of SH-SY5Y cell model with oxidative damage: SH-SY5Y cells in the logarithmic growth phase were seeded in 96-well cell culture plates, cultured overnight, and 200 μmol / L H 2 o 2 The solution was incubated with the cells for 24 hours, and the control group was set without any treatment. 10 μL of CCK-8 solution was added to each well, and the culture was terminated after 4 hours, and the absorbance at 450 nm was detected with a microplate reader. The measured cell survival rate of the model group was 73.1% of that of the control group, indicating that the oxidative damage model was successfully constructed.

[0047] Evaluation of proliferation-promoting ability: SH-SY5Y cells in the logarithmic growth phase were inoculated in 96-well cell culture plates, cultured overnight, and 200 μmol / L H 2 o 2 The solution was incubated with cells for 24h...

experiment example 3

[0049] Oral Absorption Efficiency Determination of 12 Peptides

[0050] Caco-2 monolayer cell model construction: Caco-2 cell monolayer model was selected to study the transport of representative peptides. Select Caco-2 cells in good growth state for digestion, and use 1×10 5 piece / cm 2 Inoculated on the polycarbonate membrane of the Millicell chamber, inserted the Millicell chamber into a 24-well plate, and placed it in an incubator at 37°C, 5% CO 2 cultivated in the environment. The medium was changed 24 hours after inoculation, and then every 2 days. After culturing for 21 days, the transmembrane resistance value TEER was measured. TEER values ​​are greater than 300Ω·m 2 , indicating that the Caco-2 monolayer cell model was successfully constructed.

[0051]Caco-2 cell transport experiment: First, 15 polypeptides were fluorescently labeled with 5-FAM, and a 3 mg / mL solution was prepared as a dosing solution. Then, the liquid on the top and bottom sides of the Millice...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides anti-oxidation bioactive peptides and application thereof. An amino acid sequence of the anti-oxidation active peptides comprises one or several of SEQ ID NO. 1 to SEQ ID NO. 12. The anti-oxidation active peptides provided by the invention are all derived from walnut meal hydrolysate, and each anti-oxidation bioactive peptide be obtained through solid-phase synthesis. The anti-oxidation active peptides provided by the invention are high in antioxidant activity and strong in nerve cell proliferation promotion ability, are particularly capable of remarkably promoting proliferation of SH-SY5Y cells in oxidative damage and are high in oral absorption efficiency.

Description

technical field [0001] The present invention relates to the technical field of food-derived bioactive peptides, and more specifically, to an antioxidative active peptide and its application. Background technique [0002] Certain short peptides can regulate human body functions while providing nutrients necessary for human growth and development. These biologically active peptides are called active peptides. Active peptides are important active ingredients of medicines or functional foods. Compared with proteins, peptides have a smaller molecular weight, better stability in the gastrointestinal tract, and are more conducive to absorption. Different biological peptides have different structures and physiological functions. Active peptides mainly include the following types: binding peptides, phosphopeptides, glutathione, antibacterial peptides, neuropeptides, immune peptides, flavoring peptides, hormone peptides, nutritional peptides, antibacterial peptides, oxidized peptides...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08A61K38/08A61K38/10A61P39/06A61P25/00A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P25/00A61P39/06C07K7/06C07K7/08A23V2250/55A23V2200/30A23V2200/322
Inventor 盛剑勇杨晓宇曾坚刘卫杨祥良万江陵
Owner HUAZHONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com