Method for preparing varicella virus stock and application thereof

Abstract

The invention discloses a method for preparing varicella virus stock. The method is characterized by comprising the following steps: firstly adding 5v / v percent bovine serum into a serum-free medium at the cell culture stage of MRC-5 cell, then inoculating VZV-Oka strain, and culturing virus through the serum-free medium, so as to obtain the varicella virus stock. The method uses a suitable reduced-serum medium to culture cell, cultures VZV virus in a serum-free condition, applies the virus to the preparation of varicella attenuated live vaccine, and can greatly reduce the quantity of bovine serum albumin residue in the vaccine by 60 percent or more.

Description

technical field [0001] The invention relates to a method for preparing biological products, in particular to a method for preparing varicella vaccine, which reduces the residual amount of bovine serum albumin. Background technique [0002] Bovine serum (FBS) is a mixture of complex components, the main components of which are proteins, polypeptides, hormones, and other components such as amino acids, glucose, and trace elements. Serum has extremely important functions in cell culture, such as: providing hormones that can promote cell exponential growth, nutrients that are absent or contained in basal medium, and certain low-molecular nutrients; providing binding proteins, identifying vitamins, Lipids, metal ions, and combined with toxic metals and pyrogens to detoxify; it is the source of factors required for cell adhesion and spreading; it acts as a pH buffer; and it provides protease inhibitors to make the remaining pancreas Protease inactivation protects cells from damag...

AI Technical Summary

Problems solved by technology

However, washing is a key process, if the washing is not sufficient, it will directly lead to the residue of the batch of bovine serum exceeding the standard and be discarded directly

If the washing is excessive, the diseased cells will be washed away, resulting in a low recovery rate of a certain batch of stock solution and a drop in titer, which in turn affects the yield.

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