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A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column

A technology based on polycyclic aromatic hydrocarbons and rosin, applied in the field of high-performance liquid chromatography, can solve the problems of high price, cumbersome steps, and environmental pollution, and achieve the effect of small expansion and good stability

Active Publication Date: 2021-09-24
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mobile phase acetonitrile used in this method is expensive and not environmentally friendly. The gradient elution method requires a blank gradient experiment. The steps are cumbersome and the limit is difficult to level off.
[0007] 2. Sun Yan, Yang Hongbiao, Li Chenguang, etc. Research on the detection method of polycyclic aromatic hydrocarbons in food [J]. Chinese Journal of Health Inspection, 2005,15(11):1319-1320; Extraction method: after sample pretreatment, use Florisil small column (silicon magnesium adsorbent) to purify, use benzene as solvent elution; detection method: gradient elution, mobile phase A is 50% acetonitrile aqueous solution, B is 100% acetonitrile When 0-20min, A is 100%, 20-40min, B is 100% gradually, B is 100% during 40-55min, A is 100% gradually during 55-65min, and then kept for 5min; The benzene solvent used in the extraction process not only seriously pollutes the environment, but also causes secondary pollution to food and medicine; the detection method uses acetonitrile as the mobile phase, which is expensive and has cumbersome gradient elution steps
[0008] 3. Gong Zhihua, Li Qin, Song Meihui, et al. Analysis of plant polycyclic aromatic hydrocarbons environmental toxins by high performance liquid chromatography with C18 column[J]. China Agricultural Science Bulletin, 2013, 29(32): 270-273; The chromatographic condition that polycyclic aromatic hydrocarbon is detected is to use acetonitrile and water as mobile phase equally, the mode of gradient elution, there is the same deficiency as above document
[0009] 4.Ciecierska,Obiedzinski,M.W.Polycyclic aromatic hydrocarbons invegetable oils from unconventional; sources[J].Food Control,2013,30(2):556-562; this document uses BAKERBOND PAH-16Plus chromatographic column, the mentioned polycyclic The chromatographic conditions for the detection of aromatics are also using acetonitrile and water as the mobile phase, and the gradient elution method has the same shortcomings as the above literature.
[0011] There is no report about a method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column

Method used

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  • A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column
  • A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column
  • A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column

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Embodiment 1

[0049] The prepared weakly polar rosin-based polymer chromatographic column was connected to a high-performance liquid chromatograph, washed with methanol until the baseline was balanced, and then the sample was injected.

[0050] A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column, carried out according to the following steps:

[0051] (1) Preparation of sample solution: take an appropriate amount of benzene and naphthalene, dissolve in methanol, prepare a mixed solution containing 0.05 mol of benzene and 0.005 mol of naphthalene per 1 L, and inject the sample;

[0052] (2) Setting parameters: connect the weakly polar rosin-based polymer chromatographic column to the liquid chromatograph, set the flow rate of the mobile phase of the liquid chromatograph to 0.3mL / min, the detection wavelength to 254nm, and the column oven to be 30°C;

[0053] (3) Separation: Start the injection valve to allow methanol to brin...

Embodiment 2

[0056] The prepared weakly polar rosin-based polymer chromatographic column was connected to a high-performance liquid chromatograph, washed with methanol until the baseline was balanced, and then the sample was injected.

[0057] A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column, carried out according to the following steps:

[0058] (1) Preparation of sample solution: take an appropriate amount of benzene and naphthalene, dissolve in methanol, prepare a mixed solution containing 0.05 mol of benzene and 0.005 mol of naphthalene per 1 L, and inject the sample;

[0059] (2) Setting parameters: connect the weakly polar rosin-based polymer chromatographic column to the liquid chromatograph, set the flow rate of the mobile phase of the liquid chromatograph to 0.3mL / min, the detection wavelength to 254nm, and the column oven to be 30°C;

[0060] (3) Separation: Start the injection valve to allow methanol to brin...

Embodiment 3

[0063] The prepared weakly polar rosin-based polymer chromatographic column was connected to a high-performance liquid chromatograph, washed with methanol until the baseline was balanced, and then the sample was injected.

[0064] A method for separating polycyclic aromatic hydrocarbons using a weakly polar rosin-based polymer chromatographic column, carried out according to the following steps:

[0065] (1) Preparation of sample solution: take an appropriate amount of benzene and naphthalene, dissolve in methanol, prepare a mixed solution containing 0.05 mol of benzene and 0.005 mol of naphthalene per 1 L, and inject the sample;

[0066] (2) Setting parameters: connect the weakly polar rosin-based polymer chromatographic column to the liquid chromatograph, set the flow rate of the mobile phase of the liquid chromatograph to 0.3mL / min, the detection wavelength to 254nm, and the column oven to be 30°C;

[0067] (3) Separation: Start the injection valve to allow methanol to brin...

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Abstract

The invention discloses a method for separating polycyclic aromatic hydrocarbons by using a weakly polar rosin-based polymer chromatographic column. ‑5 mol sample solution of a polycyclic aromatic hydrocarbon mixture; the polycyclic aromatic hydrocarbon mixture is a mixture of two or more of benzene, naphthalene, fluorene, pyrene, and benzopyrene; (2) setting parameters: the weakly polar rosin base The polymer chromatographic column is connected to the liquid chromatograph, and the mobile phase flow rate of the liquid chromatograph is set to 0.1-1.0mL / min, the detection wavelength is 254-270nm, and the column temperature is 30±15°C; (3) Separation: start sample injection The valve enables the mobile phase to bring the sample solution into the rosin-based polymer chromatographic column to realize the separation of the five polycyclic aromatic hydrocarbons. The invention has high sensitivity and simple operation, and does not cause secondary pollution to the separated samples.

Description

technical field [0001] The invention belongs to the field of high-performance liquid chromatography, in particular to a method for separating polycyclic aromatic hydrocarbons by using a weak polarity rosin-based polymer chromatographic column. Background technique [0002] Polycyclic aromatic hydrocarbons are an important class of environmental and food and drug pollutants, most of which are carcinogenic and can cause cancer through contact. The chemical structural formulas of five common polycyclic aromatic hydrocarbons in the field of food and drug are: [0003] Among them, benzopyrene has the greatest impact on the human body, because of its strong fat solubility, it can be directly inhaled into the human body. Polycyclic aromatic hydrocarbons are widely found in the atmosphere, water, Chinese herbal medicine and food, and are not easy to degrade. They are persistent organic pollutants, so the separation and detection of polycyclic aromatic hydrocarbons is of great s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 雷福厚王振李品礼李鹏飞王婷覃丽婷李浩程格格孙耀谢文博鄂羽羽
Owner GUANGXI UNIV FOR NATITIES
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