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Measuring method for inhibiting broad bean blight during intercropping between wheat and broad bean

A method of determination and technology of fusarium wilt, which is applied in the field of general botany, can solve the problems that are not conducive to farmers' increase in production and income and sustainable development of agriculture, low productivity of intercropping crops, and lack of inhibition, so as to promote the increase of production and income and sustainable agriculture The effect of developing and enhancing farmland biodiversity and farmland ecological stability and increasing income

Inactive Publication Date: 2018-10-19
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] (1) The existing technology lacks understanding of the inhibitory effect of root exudates on broad bean wilt in wheat and broad bean intercropping systems, resulting in low productivity of intercropping crops; low grain yield
[0005] (2) The use of a large amount of chemical fertilizers and pesticides pollutes the environment; it is not conducive to increasing farmers' production and income and sustainable agricultural development

Method used

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  • Measuring method for inhibiting broad bean blight during intercropping between wheat and broad bean
  • Measuring method for inhibiting broad bean blight during intercropping between wheat and broad bean
  • Measuring method for inhibiting broad bean blight during intercropping between wheat and broad bean

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Embodiment 1

[0046] 1, the assay method that wheat broad bean intercropping that the embodiment of the present invention provides suppresses broad bean wilt comprises:

[0047] 1) Soil cultivation of wheat broad bean intercropping and broad bean monoculture and inoculation of pathogenic bacteria

[0048] Soil culture test of wheat and broad bean intercropping and broad bean single cropping: the size of the test pot is 14×20cm, and each pot is filled with 2.5kg of soil (the non-continuous cropping soil of broad bean is collected in the field, and the basic agrochemical properties of the soil are organic matter content of 23.2g.kg -1 , total nitrogen 1.90g.kg -1 , Alkaline hydrolyzed nitrogen 119.0mg.kg -1 , available phosphorus 56.5mg.kg -1 , available potassium 123.4mg.kg -1 , pH value is 6.4); planting density of wheat broad bean: 4 seedlings per pot for single-cropping broad bean, 2 seedlings for wheat broad bean intercropping broad bean, and 4 wheat seedlings (for the test varieties:...

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Abstract

The invention belongs to the technical field of botany, and discloses a measuring method for inhibiting broad bean blight during the intercropping between wheat and broad bean. The method comprises following steps: carrying out earth culture of intercropping between wheat and broad bean and sole cropping of broad bean, inoculating pathogenic bacteria; collecting the rhizosphere soil and root secretion, investigating the diseases; measuring the sugar, amino acids, and organic acids of the root secretion; and evaluating the influence of the root secretion on spore germination, mycelium growth, and spore production of fusarium oxysporum. The provided method can be used to explain the mechanism of soil-borne diseases of intercropping; can increase the yields of intercropping and biological diversity of farmland, improves the ecological stability, and is environmentally friendly. High yield of crops is realized. The bad influence of fertilizers and pesticides on the environment is eliminated. The incomes of farmers are increased. The method has important guiding significance and application value for sustainable agriculture.

Description

technical field [0001] The invention belongs to the technical field of general botany, and in particular relates to a method for determining the suppression of broad bean wilt by wheat and broad bean intercropping. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: The pursuit of large-scale planting of single crops in modern agriculture and the large-scale application of pesticides and fertilizers have resulted in the increasingly simple and fragile farmland ecosystem, resulting in frequent occurrence of crop diseases and shorter and shorter disease epidemic cycles, resulting in reduced crop production, and at the same time caused continuous depletion of non-renewable resources and serious problems such as environmental pollution. As the essence of traditional agriculture in my country, intercropping has the advantages of increasing production and improving the efficiency of nutrient resource utilization. It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 董艳董坤肖靖秀朱锦惠张仕颖
Owner YUNNAN AGRICULTURAL UNIVERSITY
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