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Saline-alkali soil repairing microbial inoculum and preparation method thereof

A microbial agent and saline-alkali soil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of general improvement effect, inability to reduce the alkalinity of saline-alkali land, and aggravate the degree of salinization, and achieve Improve the emergence rate and pest resistance, reduce the content of soluble inorganic salts, reduce the effect of salt content and PH value

Inactive Publication Date: 2018-10-19
汤永红
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the improvement methods of saline-alkali soil mainly include physical irrigation and salt washing, chemical neutralization, microbial improvement and other forms. Among them, physical irrigation and water washing of salt only disperses the salt in saline-alkali soil to other areas. It is slow and consumes a lot of manpower and material resources. Chemical neutralization is to use acidic reagents to reduce the pH value of saline-alkali land, but it is easy to cause soil compaction and destroy microbial flora. The improvement of microbial bacterial agents can not only promote the decomposition of soil organic matter and crop metabolism, but also reduce the obstacles of repeated cropping. And it can degrade chemical fertilizers and pesticide residues, improve crop quality and yield, but the anti-osmotic pressure ability of microorganisms is generally low, and the mortality rate is high in high-concentration saline-alkali soil, so the improvement effect is general
[0003] The patent application number is CN201711194015.6, which discloses a microbial fertilizer for the improvement of saline-alkali soil in alkaline soil. By compounding E. The components are mixed to obtain the microbial fertilizer, but the function of the phosphorus-dissolving potassium-dissolving bacteria is to decompose the insoluble inorganic minerals in the soil into soluble potassium, phosphorus, magnesium and other elements, which will increase the metal ion content in the saline-alkali soil and further aggravate the pollution. The nitrogen-fixing bacteria convert the nitrogen in the atmosphere into nitrate and nitrite components, which also further deteriorates the saline-alkali soil, and the microorganisms in the microbial agent have low osmotic pressure resistance and survive in saline-alkali soil In addition, microbial fertilizers cannot reduce the alkalinity of saline-alkali land

Method used

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  • Saline-alkali soil repairing microbial inoculum and preparation method thereof
  • Saline-alkali soil repairing microbial inoculum and preparation method thereof
  • Saline-alkali soil repairing microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The percentage by weight of each component of the saline-alkali soil repairing microbial agent is as follows: 25% of the microbial immobilization carrier, 55% of the fermented liquid of the functional microorganism, and 20% of the fermented liquid of the acid-producing microorganism.

[0029] The weight ratio of the components of the above-mentioned immobilization carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose = 1:0.2:0.7:1.6.

[0030] The concentration of Desulfosarcina in the above-mentioned functional microbial fermentation liquid is higher than 5×10 7 CUF / ml, the concentration of denitrifying phosphorus accumulating bacteria is higher than 4×10 9 CUF / ml, Shewanella concentration higher than 2×10 9 CUF / ml, the concentration of Trichobacter fluorescens is higher than 6×10 8 CUF / ml, Bacillus licheniformis concentration higher than 8×10 7 CUF / ml.

[0031] The concentration of Gluconobacter oxidans in the fermentation liquid of the above-men...

Embodiment 2

[0040] The percentage by weight of each component of the saline-alkali soil repairing microbial agent is as follows: 30% of a microbial immobilization carrier, 45% of a functional microbial fermentation broth, and 25% of an acid-producing microbial fermentation broth.

[0041] The weight ratio of the components of the above immobilization carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose = 1:0.25:0.75:1.8.

[0042] The above-mentioned sulfate-reducing bacteria are Desulfomonas bacteria.

[0043] The pH value of the acidogenic microorganism fermentation broth is 3.5.

[0044] The preparation method of above-mentioned saline-alkali soil remediation microbial bacterial agent is the same as embodiment 1, the difference is:

[0045] The difference in composition of the media produced by S1 and S2 is shown in Table 1:

[0046] Table 1

[0047]

[0048] S3: medical stone crushing particle size is 60 mesh; S4: He-Ne laser current intensity is 20-40mA, radi...

Embodiment 3

[0050] The percentage by weight of each component of the saline-alkali soil repairing microbial agent is as follows: 35% of microbial immobilization carrier, 35% of functional microbial fermentation liquid, and 30% of acid-producing microbial fermentation liquid.

[0051] The weight ratio of the components of the above-mentioned immobilization carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose = 1:0.3:0.8:2.

[0052] The above-mentioned sulfate-reducing bacteria are Desulfofinomycetes.

[0053] The pH value of the acidogenic microorganism fermentation broth is 4.5.

[0054] The preparation method of above-mentioned saline-alkali soil remediation microbial bacterial agent is the same as embodiment 1, the difference is:

[0055] The difference in composition of the medium produced by S1 and S2 is shown in Table 2:

[0056] Table 2

[0057]

[0058] S3: medical stone crushing particle size is 80 mesh; S4: He-Ne laser current intensity is 40mA, radiatio...

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Abstract

The invention discloses a saline-alkali soil repairing microbial inoculum and a preparation method thereof. The microbial inoculum is obtained by adsorbing and embedding a functional microorganism fermentation solution and an acid-producing microorganism fermentation solution through an immobilized carrier, wherein the microorganism immobilized carrier comprises medical stones, citric acid, carrageenan and carboxyethyl cellulose; the functional microorganism fermentation solution is prepared by carrying out anaerobic fermentation on sulfate-reducing bacteria, denitrifying phosphate-accumulating bacteria, shewanella, pseudomonas fluorescens and bacillus licheniformis, which are screened through He-Ne laser induced screening; the acid-producing microorganism fermentation solution is obtainedby carrying out aerobic fermentation on gluconobater oxydans, rhizopus oryzae and aspergillus niger. According to the saline-alkali soil repairing microbial inoculum, the microorganism immobilized carrier is used for adsorbing and embedding the microorganism fermentation solution so that microorganisms are gradually diluted to saline-alkali soil and the tolerance of the microorganisms is improved; He-Ne laser induced breeding is utilized and the resistance capability of the microorganisms on bad factors is improved, so that the microorganism activity and the repairing capability are improved.

Description

technical field [0001] The invention relates to the technical field of saline-alkali land improvement, in particular to a saline-alkali soil restoration microbial agent and a preparation method thereof. Background technique [0002] Due to the redistribution of various soluble salts in the horizontal and vertical directions on the surface, the salt is continuously accumulated on the soil surface in the salt-collecting area, forming saline-alkali soil that endangers the growth of crops. According to incomplete statistics from UNESCO and the Food and Agriculture Organization, the area of ​​saline-alkali land in China is about 99.13 million hectares, and the degree of salinization is generally high, making it difficult for crops to survive. At present, the improvement methods of saline-alkali soil mainly include physical irrigation and salt washing, chemical neutralization, microbial improvement and other forms. Among them, physical irrigation and water washing of salt only dis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N11/14C12N11/02C12N11/12C09K17/40C09K109/00C12R1/01C12R1/10C12R1/845C12R1/685
CPCC09K17/40C12N1/14C12N1/20C12N11/02C12N11/12C12N11/14
Inventor 汤永红
Owner 汤永红
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