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Adhesive composition for coating of glass slide and single-surface coating technology

A technology of glass slides and adhesives, applied in the direction of coatings, adhesives, adhesive types, etc., can solve problems such as uneven quality of coating products, uneven laying of coatings, and non-continuous batch production, etc., to achieve The effect of low price, uniform laying and gentle production process

Active Publication Date: 2018-10-12
HANGZHOU YIGUOREN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another object of the present invention is to provide a novel coating process in which the adhesive can be uniformly coated on the glass slide, designated area coating, and continuous production, so as to overcome the traditional immersion method coating process that causes the adhesive on the glass slide The coating is unevenly laid, the quality of the front and rear coating products is not uniform, manual operation, and the obvious shortcomings of continuous mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Weigh or measure each component according to the following, and prepare a 100ml solution

[0033] Film former: polyvinyl alcohol (PVA) 0.05 g

[0034] Binder: Carboxymethylcellulose (CMC) 0.02 g

[0035] Cationic swelling agent: polylysine (PLL) 0.2 mg

[0036] Solvent: purified water to make up 100%

[0037] Take 80ml of purified water, add 0.05g of polyvinyl alcohol (PVA), heat up to 80°C, stir to dissolve, cool to 55°C, add 0.02g of carboxymethyl cellulose (CMC), stir to dissolve at 55°C, add purified water Make up 100ml, adjust the pH to 6.2, and filter through a 200-mesh sieve to obtain the adhesive "component 1". Take 80ml of purified water, add 0.2 mg of polylysine (PLL), stir to dissolve, add purified water to make up 100ml, adjust the pH to 7.0, and filter through a 200-mesh sieve to obtain the adhesive "component 2".

[0038] According to the SOP requirements for spraying adhesive slides, prepare 300 slides after cleaning and drying, adjust the "automatic ...

Embodiment 2

[0041] Weigh or measure each component according to the following, and prepare a 100ml solution

[0042] Film-forming agent: polyvinyl alcohol (PVA) 0.10 g

[0043] Binder: Carboxymethylcellulose (CMC) 0.06 g

[0044] Cationic swelling agent: polylysine (PLL) 0.8 mg

[0045] Solvent: purified water to make up 100%

[0046] Take 80ml of purified water, add 0.10 g of polyvinyl alcohol (PVA), heat up to 85°C, stir to dissolve, cool to 60°C, add 0.06g of carboxymethyl cellulose (CMC), stir to dissolve at 58°C, add purified water Make up 100ml, adjust the pH to 7.0, and filter through a 200-mesh sieve to obtain the adhesive "component 1". Take 80ml of purified water, add 0.8 mg of polylysine (PLL), stir to dissolve, add purified water to make up 100ml, adjust the pH to 6.5, and filter through a 200-mesh sieve to obtain the adhesive "component 2".

[0047] Prepare 300 glass slides after washing and drying according to the SOP requirements for spraying of adhesive slides, adjust ...

Embodiment 3

[0050] Weigh or measure each component according to the following, and prepare a 100ml solution

[0051] Film former: polyvinyl alcohol (PVA) 0.16 g

[0052] Binder: Carboxymethylcellulose (CMC) 0.20 g

[0053] Cationic swelling agent: polylysine (PLL) 0.4 mg

[0054] Solvent: purified water to make up 100%

[0055] Take 80ml of purified water, add 0.16 g of polyvinyl alcohol (PVA), heat up to 88°C, stir to dissolve, cool to 52°C, add 0.20 g of carboxymethyl cellulose (CMC), stir to dissolve at 50°C, add purification Make up 100ml of water, adjust the pH to 6.5, and filter through a 200-mesh sieve to obtain the adhesive "component 1". Take 80ml of purified water, add 0.4 mg of polylysine (PLL), stir to dissolve, add purified water to make up 100ml, adjust the pH to 6.0, and filter through a 200-mesh sieve to obtain the adhesive "component 2".

[0056] According to the SOP requirements for spraying adhesive slides, prepare 300 slides after washing and drying, adjust the "au...

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PUM

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Abstract

The invention relates to an adhesive composition for coating of a glass slide and a single-surface coating technology. The adhesive composition is prepared from the following components according to amass / volume ratio: a component 1 and a component 2, wherein the component 1 is prepared from 0.05 to 0.3% of film forming agent (polyvinyl alcohol), 0.02 to 0.25% of adhesive (carboxymethylcellulose), and the balance of water; the component 2 is prepared from 0.2 to 1mg / 100ml of cationic swelling agent (polylysine), and the balance of water. The single-surface coating technology comprises the following steps of cleaning the glass slide by ultrasonic waves in an alkaline solution, drying, spraying and coating the component 1 to the front surface according to the amount of 20 to 50mu l per piece, drying at the temperature of 35 to 55 DEG C, cooling to room temperature, spraying and coating the component 2 to the same surface according to the amount of 10 to 30mu l per piece, drying at the temperature of 35 to 55 DEG C, and packaging under the vacuum condition. The adhesive composition has the advantages that the effects of high adhesivity, uniform distribution and high definition of cells or tissues on the glass slide are realized; the continuous, automatic, stable, uniform and batch type single-surface zonal spraying and coating technology is realized. The prepared pathology slidecan resist the repeated washing and soaking by various alcohols and reagents, be suitable for the wall attaching, single-layer distribution and no falling of the cells, the tissues and the like, and can reach the effects of clear background and no impurity.

Description

technical field [0001] The invention relates to an adhesive composition and an adhesive coating process for improving cell or tissue adhesion and high definition of pathological glass slides, in particular to a glass slide adhesive composition and an adhesive coating process. Its preparation method, and the preparation method of the coating process on the glass slide. Background technique [0002] In clinical pathology, tissue slices and liquid-based thin-layer cells are widely used in cytomorphological examination. Thin-layer cell slices and tissue slices are used to achieve the application of cell / histological examination of corresponding diseases and identification of clinical lesions has become increasingly common. In terms of pathological examination, the systemic and in-depth research on the adhesion and detachment resistance of cell thin layers or tissue sections to glass slides is not very extensive or in-depth, but adhesion to slides or detachment prevention The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J129/04C09J101/28C09J11/08B05D7/24B05D1/02B05D5/10
CPCB05D1/02B05D5/10B05D7/24C08L2205/03C09J11/08C09J101/286C09J129/04C08L1/286C08L77/04C08L29/04
Inventor 赵爱景
Owner HANGZHOU YIGUOREN BIOTECH CO LTD
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