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New application of guava leaves and total flavones of guava leaves

A technology of guava leaves and total flavonoids, which is applied in the field of medicine, can solve the problems of no relevant literature and patents on the prevention and treatment of pancreatitis, and achieve the effects of stable and feasible extraction process, stable detection method, and controllable quality

Active Publication Date: 2018-10-09
天津市医药科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology is mainly aimed at the hypoglycemic effect of total flavonoids of guava leaves, and some literatures have reported its antitumor activity, but there are no relevant literatures and patents for the prevention and treatment of pancreatitis.

Method used

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  • New application of guava leaves and total flavones of guava leaves
  • New application of guava leaves and total flavones of guava leaves
  • New application of guava leaves and total flavones of guava leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Extraction and content determination of guava leaf total flavonoids

[0017] Ethanol reflux extraction-recovery reagent under reduced pressure-concentration-filtration-petroleum ether treatment-ethyl acetate extraction and other steps for extraction. Determination of TFPGL content Use rutin as a standard substance to measure the absorbance value, take the concentration as the abscissa, and the absorbance as the ordinate, and draw a standard curve. Measure the absorbance at 500nm of the tested sample by ultraviolet spectrophotometry (rutin standard has a strong absorbance at 500nm, which is set as the measurement wavelength), and substitute it into the standard curve regression equation to calculate the total flavonoid content as 19.8%.

Embodiment 2

[0018] The acute toxicity of embodiment 2 guava leaf total flavonoids

[0019] 1. Experimental method:

[0020] Experimental Design Administration group and blank control group, 24 animals in each group, half male and half male. The administration dose of the administration group was 22.32g / kg, and the administration volume was 40ml / kg for intragastric administration. The control group was given equal volume of drinking water by intragastric administration.

[0021] The appearance, mental state, respiration, skin coat, feces and urine, eyes, ears, nose, mouth, genitals and other general conditions of the mice were observed and recorded daily, as well as other poisoning manifestations and death conditions. The body weight and food intake of the mice were measured once a week for 14 consecutive days.

[0022] 2. Experimental results:

[0023] General condition and death observation: After administration until the 14th day, all animals were in good mental and behavioral condi...

Embodiment 3

[0025] Example 3 Multi-index response surface method to optimize the assisted extraction process of total flavonoids from guava leaves

[0026] 1. Chromatographic conditions and methods:

[0027] The chromatographic column is Agilent Eclipse XDB-C18 (4.6mm×250mm, 5μm); A (methanol %), B (0.2% phosphoric acid aqueous solution %); gradient elution, 0~20.00min, 37%A; 20.01~35.00min, 70% A; volume flow rate: 1mL / min; column temperature: 35°C; detection wavelength: 360nm. Precisely draw 10 μL of the test solution, and determine hyperoside, quercetin-3-O-β-D-arabinopyranoside, quercetin-3-O-α-L-furan arabinoside in the test solution Glycoside peak area, calculate component content, combine these 3 component chromatographic peak areas to account for the percentage example of total peak area, convert the content of guava leaf total flavonoids in the test solution.

[0028] Total flavonoid content = total flavonoid content (mg / g) = sum of mass fractions of 3 kinds of flavonoid glycos...

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Abstract

The invention relates to application of total flavones of guava leaves in preparation of drugs for prevention and treatment of pancreatitis. The invention further provides a pharmaceutical compositionfor prevention and treatment of pancreatitis. The pharmaceutical composition comprises an effective therapeutic dose of total flavones of guava leaves as an active component and a pharmaceutically acceptable carrier. Animal experiments show that the total flavones of the guava leaves can inhibit the excessive proliferation of pancreatic stellate cells and alleviate the degree of pancreatic fibrosis by inhibiting the activation of the P2X7R-mediated NLRP3 inflammation signal pathway, have good effects on prevention and treatment of pancreatitis, have no toxic and side effects and can be used for preparing drugs for prevention and treatment of pancreatitis.

Description

technical field [0001] The invention relates to the field of medicine, in particular, the invention relates to a traditional Chinese medicine for preventing and treating pancreatitis and its total flavonoid extract. Background technique [0002] Pancreatitis can be acute (new, short-term) or chronic (persistent, long-term). [0003] Acute pancreatitis (AP) is a common disease causing significant morbidity and mortality. Organ failure and systemic inflammatory response syndrome occur in the early stage of the disease, and infection of necrotic tissue occurs in the late stage. At present, the treatment and prognosis of acute pancreatitis are still challenging, with the characteristics of high mortality, high disability rate, and high cost. Acute pancreatitis usually begins immediately after damage to the pancreas begins. The episodes are usually very mild. Mild episodes last for a short time and usually resolve completely when the pancreas returns to its normal state. Repeat...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P1/18
CPCA61K36/61A61P1/18
Inventor 张桂贤刘洪斌刘大卫赵秀梅
Owner 天津市医药科学研究所
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