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Method for preparing xylo-oligosaccharide and fermentable sugars through lignocelluloses-type biomass

A technology of lignocellulosic and xylooligosaccharides, applied in the field of preparation of xylooligosaccharides and fermentable sugars, can solve the problems of large environmental pollution and unsuitable for large-scale industrial production, achieve environmental friendliness, improve hydrolysis efficiency and glucose Conversion rate, save the effect of high cost

Inactive Publication Date: 2018-09-28
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sodium chlorite method can selectively remove lignin and retain cellulose and hemicellulose, the sodium chlorite reagent causes serious environmental pollution and is not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Put the crushed and dried bagasse in a glass reactor, add 0.5% sodium hydroxide and 1% hydrogen peroxide, the solid-liquid ratio is 1:10 (g:g), and the reaction temperature is 70°C, the reaction time is 4h, and after filtration, the solid residue is washed with water until neutral to obtain the pretreated substrate. Disperse the pretreated substrate with pH 5 buffer solution, then add 300 IU of glycoside hydrolase 10 family endoxylanase per gram of dry substrate, react at 45°C for 24 hours for enzymatic hydrolysis, and filter to obtain oligomeric xylanase Sugar solution and solid residue after endoxylanase hydrolysis. Disperse the obtained solid residue after xylanase hydrolysis with pH 5 buffer solution, then add 15 IU cellulase per gram of dry substrate, react at 50°C for 72 hours for enzymatic hydrolysis, and filter to obtain a fermentable sugar solution. The conversion rate of xylo-oligosaccharide was 21.12%, the conversion rate of glucose was 81.25%, and the conve...

Embodiment 2

[0020] Put the crushed and dried bagasse in a glass reactor, add 1% sodium hydroxide and 5% hydrogen peroxide, the solid-liquid ratio is 1:35 (g:g), and the reaction temperature is 30°C, the reaction time is 24h, and after filtration, the solid residue is washed with water until neutral to obtain the pretreated substrate. Disperse the pretreated substrate with pH 5 buffer solution, then add 400 IU of glycoside hydrolase 10 family endoxylanase per gram of dry substrate, react at 50°C for 12 hours for enzymatic hydrolysis, and filter to obtain oligomeric xylanase Solid residue after hydrolysis of sugar solution and xylanase. Disperse the obtained solid residue after xylanase hydrolysis with pH 5 buffer solution, then add 25 IU cellulase per gram of dry substrate, react at 50°C for 48 hours for enzymatic hydrolysis, and filter to obtain a fermentable sugar solution. The conversion rate of xylo-oligosaccharide was 20.05%, the conversion rate of glucose was 82.26%, and the convers...

Embodiment 3

[0022] Put the crushed and dried bagasse in a glass reactor, add 1% sodium hydroxide and 1.5% hydrogen peroxide, the solid-liquid ratio is 1:25 (g:g), and the reaction temperature is 90°C, the reaction time is 1h, and after filtration, the solid residue is washed with water until neutral to obtain the pretreated substrate. Disperse the obtained pretreatment substrate with pH 5 buffer solution, then add 600 IU of glycoside hydrolase 11 family endoxylanase per gram of dry base substrate, react at 55°C for 9 hours for enzymatic hydrolysis, and filter to obtain oligomeric xylanase Solid residue after hydrolysis of sugar solution and xylanase. Disperse the obtained solid residue after xylanase hydrolysis with pH 5 buffer solution, then add 20 IU cellulase per gram of dry substrate, react at 50°C for 96 hours for enzymatic hydrolysis, and filter to obtain a fermentable sugar solution. The conversion rate of xylo-oligosaccharide was 23.65%, the conversion rate of glucose was 85.14%,...

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Abstract

The invention discloses a method for preparing xylo-oligosaccharide and fermentable sugars through lignocelluloses-type biomass. Firstly, oxidation pretreatment is conducted under the alkaline condition to selectively remove lignin in the lignocelluloses-type biomass; then endo-xylanase which does not have cellulase activity is adopted to conduct hydrolysis pretreatment on a substrate to obtain the xylo-oligosaccharide; and finally, cellulase is adopted to hydrolyze solid residues after the endo-xylanase is hydrolyzed to obtain the fermentable sugars. According to the method, the xylan separation and extraction processes where energy consumption is high, consequently the cost is high, the dosage of chemical reagents is large, and consequently, pollution is serious are omitted, the purity of the xylo-oligosaccharide is further improved, and the yield of the fermentable sugars is increased.

Description

Technical field: [0001] The invention relates to the field of biochemical technology, in particular to a method for preparing xylooligosaccharides and fermentable sugars from lignocellulosic biomass. Background technique: [0002] Xylo-oligosaccharides are low-molecular-weight xylooligosaccharides with special physiological functions, because they can promote the proliferation of beneficial bacteria such as lactic acid bacteria and bifidobacteria, inhibit the growth of pathogenic bacteria such as Clostridium and Escherichia coli, and have the ability to regulate intestinal flora Balance and improve bowel function. Xylo-oligosaccharide is therefore recognized as a good prebiotic with high market value. The raw materials for the production of xylooligosaccharides are mainly lignocellulosic biomass such as corncobs, bagasse and straw. At present, the conventional method for preparing xylo-oligosaccharides needs to separate xylan from lignocellulose, and then use xylanase to h...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/02C12P19/12C12P19/14
CPCC12P19/02C12P19/04C12P19/12C12P19/14C12P2201/00
Inventor 陈新德黎海龙熊莲陈雪芳黄超王璨
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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