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Paenibacillus kribbensis YB326 and application thereof

A Bacillus and jelly-like technology, which is applied to jelly-like Paenibacillus and its application fields in rice planting and animal breeding, can solve the problems of environmental pollution, high prevention and control costs, long time consumption and the like, and achieves improved rice yield. , Improve stress resistance, reduce the effect of pests and diseases

Active Publication Date: 2018-09-25
云南博仕奥生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are problems such as high cost of prevention and control, long time consumption, and environmental pollution

Method used

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  • Paenibacillus kribbensis YB326 and application thereof
  • Paenibacillus kribbensis YB326 and application thereof
  • Paenibacillus kribbensis YB326 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Isolation and screening of jelly-like Paenibacillus kribbensis YB326

[0039] Sample collection: Take soil from a garden in Guangzhou City, Guangdong Province, use a sterile shovel to remove the top soil, use a sterile sampler to collect soil at a depth of 5 to 10 cm below the surface, collect 5 samples at each point, and put them into a sterile bag , brought back to the laboratory for strain isolation.

[0040] Soil dilution preparation: Weigh 10g of soil, put it into a triangular flask filled with 90mL sterile water and shake it fully to make a dilution of 10 -1 soil dilution. Then, serially dilute to prepare a dilution of 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 soil solution for use.

[0041] Bacteria isolation: take 10 -4 、10 -5 and 10 -6 Three dilutions of the soil suspension were each 0.1 mL, dropped into the LB medium, and the suspension was evenly spread with a sterile applicator, and cultured upside down at 37°C, and three replicate experiment...

Embodiment 2

[0044] Embodiment 2: Identification of jelly-like Paenibacillus kribbensis YB326

[0045] The colony morphological characteristics of strain YB326 are as follows: figure 1 Shown: It grows well in Chase's medium, the surface of the colony is smooth, milky white, mucus-like, with protrusions in the middle. The microscopic morphological characteristics of the bacteria YB326 are as follows: figure 2 , rod-shaped, capsulated, bacterium 0.8 ~ 1.2μm.

[0046] The jelly-like Paenibacillus kribbensis YB326 16S rDNA gene was sequenced, and the sequence was compared and analyzed by Blast. It was found that the strain YB326 belonged to the genus Paenibacillus (Paenibacillus), and the similarity with the typical strain of the genus Paenibacillus kribbensis was 99%. According to the morphological characteristics of the strain, it can be judged that the strain YB326 is identified as Paenibacillus kribbensis, and its phylogenetic tree is shown in image 3 .

[0047] Therefore, the strain...

Embodiment 3

[0049] Embodiment 3: Shake flask fermentation

[0050] Seed medium: PDA medium

[0051] Fermentation medium: sucrose 30g / L, beef extract 3g / L, K 2 HPO 4 ·7H 2 O 2g / L, MgSO 4 ·7H 2 O0.5g / L, initial pH 7.2, solvent is water.

[0052] The Paenibacillus kribbensis YB326 that inoculates the activated jelly-like bacillus Paenibacillus kribbensis YB326 is cultivated in the seed medium for 24h to make the seed liquid, then the seed liquid is inoculated into the 250mL Erlenmeyer flask that 50mL fermentation medium is housed, and the inoculum size is 5% (v / v ), temperature 37°C, 250r / min shaking table fermentation culture for 72h, adding 2 times the volume of absolute alcohol to precipitate polysaccharides, the content is 10g / L, and the supernatant is evaporated to dryness to obtain oligosaccharides, the content is 8g / L.

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Abstract

The invention discloses paenibacillus kribbensis YB326 and application thereof. According to the paenibacillus kribbensis YB326 and the application thereof, the preservation number of the paenibacillus kribbensis YB326 is GDMCC NO:60326; the bacterial strain of the paenibacillus kribbensis YB326 can generate anti-fungal natural active substances and extracellular active polysaccharides and oligosaccharides, and fermentation metabolites of the paenibacillus kribbensis YB326 have a relatively strong inhibition effect on ten kinds or more of plant germs of rice sheath blight disease, rice blast and the like; active carbohydrates comprise fructan and fructooligosaccharide; on the one hand, the fermentation metabolites not only can efficiently prevent and treat the rice sheath blight disease and the rice blast, but also can improve the resistance of rice, improve the rice yield, has a wide application prospect and has great significance in reducing the use of a large amount of chemical fertilizers and pesticides in rice planting and reducing the agricultural non-point source pollution; and on the other hand, the extracted extracellular active polysaccharides and oligosaccharides are added into fodders, so that the animal immunity can be effectively enhanced, the animal intestinal health can be improved, and the animal production performance can be improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Paenibacillus jelly-like strain and its application in rice planting and animal breeding. Background technique [0002] Rice is the most important food crop in my country. Among the three major food crops, as the main ration variety, the output of rice accounts for nearly 40% of the output of the three major food crops. There are many factors affecting rice production, among which disease is one of the important obstacles restricting rice production. There are many kinds of rice diseases, and there are nearly a hundred kinds reported all over the world. There are more than 70 species recorded in my country, which are relatively common in production, and more than 20 species of diseases have caused important economic losses, among which rice blast, sheath blight and bacterial blight have the largest occurrence area, the highest prevalence frequency, and the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P19/04C12P19/00A01N63/02A01P3/00A01G7/06A01G13/00A23K20/163A23K10/16C12R1/01
CPCA01G7/06A01G13/00A23K10/16A23K20/163A01N63/10C12P1/04C12P19/00C12P19/04C12N1/205C12R2001/01
Inventor 梁锏文廖美德杜景德李伟
Owner 云南博仕奥生物技术有限公司
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