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Porous gelatin sponge and preparation method thereof

A gelatin sponge and gelatin technology, applied in the field of biomedical materials, can solve problems such as inability to obtain porous gelatin hemostatic materials, and achieve the effects of shortening clinical hemostasis time, increasing porosity, and increasing the formation of pores

Active Publication Date: 2018-09-14
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used pore-making methods include particle dissolution method, phase separation method, freeze-drying method, gas foaming method, sintering microsphere method, etc., but none of them can obtain a porous gelatin hemostatic material with a high porosity. How to choose a suitable pore-making method method, it is still a difficult problem to prepare a high-porosity, and has a good hydrophilic superabsorbent hemostatic material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of porous gelatin sponge, comprising the steps of:

[0030] S1. In a 1000mL glass beaker, add 250mL of water, add 0.01g of glutaraldehyde, and stir to completely dissolve the glutaraldehyde in the water. Slowly add 20g of gelatin with a jelly strength of 300 Bloom / g into the beaker, stir to disperse the gelatin evenly in the solution, seal the mouth of the beaker with plastic wrap, then place the beaker in a water bath and heat it to 50°C for 4 hours ;

[0031] S2. Transfer the cross-linked gelatin solution to a specific airtight reaction vessel, inject hydrogen gas into the gelatin solution, pressurize the reaction vessel to 2 atmospheres, and maintain the pressure for 2 hours.

[0032] S3. programmatically cool the gelatin solution in the closed reaction vessel to -80°C, at a cooling rate of 10°C / h, so that the solution turns into a solid;

[0033] S4. Freeze-drying the solidified gelatin using a low-temperature and low-pressure freeze dryer;

...

Embodiment 2

[0037] A preparation method of porous gelatin sponge, comprising the steps of:

[0038] S1. In a 1000mL glass beaker, add 250mL of water, add 0.01g of glutaraldehyde, and stir to completely dissolve the glutaraldehyde in the water. Slowly add 20g of gelatin with a jelly strength of 300 Bloom / g into the beaker, stir to disperse the gelatin evenly in the solution, seal the mouth of the beaker with plastic wrap, then place the beaker in a water bath and heat it to 50°C for 4 hours ;

[0039] S2. Transfer the cross-linked gelatin solution to a specific airtight reaction vessel, inject hydrogen gas into the gelatin solution, pressurize the reaction vessel to 2 atmospheres, and maintain the pressure for 2 hours.

[0040] S3. programmatically cool the gelatin solution in the closed reaction vessel to -80°C, at a cooling rate of 5°C / h, so that the solution turns into a solid;

[0041] S4. Freeze-drying the solidified gelatin using a low-temperature and low-pressure freeze dryer;

...

Embodiment 3

[0045] S1. In a 1000mL glass beaker, add 100mL of water, add 0.1g of EDC, stir to completely dissolve the EDC in the water. Slowly add 10g of gelatin with a jelly strength of 100 Bloom / g into the beaker, stir to disperse the gelatin evenly in the solution, seal the mouth of the beaker with plastic wrap, then place the beaker in a water bath and heat it to 60°C for 12 hours ;

[0046] S2. Transfer the cross-linked gelatin solution to a specific airtight reaction vessel, inject hydrogen gas into the gelatin solution, pressurize the reaction vessel to 4 atmospheres, and maintain the pressure for 1.5 hours.

[0047] S3. programmatically cool the gelatin solution in the closed reaction vessel to -80°C at a cooling rate of 20°C / h, so that the solution turns into a solid;

[0048] S4. Freeze-drying the solidified gelatin using a low-temperature and low-pressure freeze dryer;

[0049] S5. Soak the porous gelatin sponge freeze-dried in step S4 in absolute ethanol and purified water f...

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Abstract

The invention discloses a preparation method of porous gelatin sponge. The preparation method comprises the following steps: S1, dissolving a crosslinking agent; adding gelatin in a stirring process and carrying out crosslinking reaction; S2, transferring a cross-linked gelatin solution into a sealed reaction container; introducing gas until the pressure in the reaction container reaches one atmospheric pressure or more, and then keeping for 10min or more; S3, carrying out programmed cooling on the gelatin solution treated by step S2 to be completely cured; S4, freezing and drying the cured gelatin; S5, immersing the freeze-dried gelatin into absolute ethyl alcohol and purified water in sequence to remove the crosslinking agent; S6, carrying out secondary freeze-drying on the immersed gelatin in step S5 and sterilizing to obtain the porous gelatin sponge. According to the preparation method of the porous gelatin sponge, disclosed by the invention, gas introduction and pressurizing, andprogrammed cooling curing treatment are carried out after the gelatin is crosslinked, and the inner porosity and specific surface area of the gelatin sponge are effectively increased; the water absorption performance of the gelatin sponge is improved so that the bleeding-stopping speed of the gelatin sponge is improved and the bleeding-stopping time is shortened.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a porous gelatin sponge and a preparation method thereof. Background technique [0002] Most of the hemostatic materials have a very high absorption of liquid during the hemostasis process. The adsorption of water in the blood will lead to the concentration of platelets, thereby enhancing the aggregation speed and ability of platelets. The rapid water absorption will also reduce the wound site. The volume of liquid is more conducive to the occurrence of platelet aggregation. After the material absorbs water and swells, it will form a certain pressure on the surrounding blood vessels to further promote hemostasis; after absorbing water, it will form a hydrogel, which can be blocked on the wound surface and increase the effect of hemostasis. [0003] Gelatin is a kind of macromolecular hydrocolloid, which is widely used in medicine and chemical industry, and has good ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J3/24A61L15/32A61L15/42A61L15/44A61L15/60A61L15/64C08L89/00
CPCA61L15/325A61L15/425A61L15/44A61L15/60A61L15/64A61L2400/04C08J3/24C08J9/28C08J2389/00C08L89/00
Inventor 林丽敏邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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